Haplotype structure of the beta2-adrenergic receptor gene in 814 Danish Caucasian subjects and association with body mass index

Mette Kamp Jensen; Morten Nielsen; Pernille Koefoed; Henning Bay Nielsen; Henrik Ullum; Eva Haastrup; Bertil Romner; Finn Borgbjerg Moltke; Niels Vidiendal Olsen; Mette Kamp Jensen; Morten Nielsen; Pernille Koefoed; Henning Bay Nielsen; Henrik Ullum; Eva Haastrup; Bertil Romner; Finn Borgbjerg Moltke; Niels Vidiendal Olsen

doi:10.3109/00365510903301536

Haplotype structure of the beta2-adrenergic receptor gene in 814 Danish Caucasian subjects and association with body mass index

Mette Kamp Jensen, Morten Nielsen, Pernille Koefoed, Henning Bay Nielsen, Henrik Ullum, Eva Haastrup, Bertil Romner, Finn Borgbjerg Moltke, Niels Vidiendal Olsen, Mette Kamp Jensen, Morten Nielsen, Pernille Koefoed, Henning Bay Nielsen, Henrik Ullum, Eva Haastrup, Bertil Romner, Finn Borgbjerg Moltke, Niels Vidiendal Olsen

Department of Clinical Medicine

3 Citations (Scopus)

Abstract

Several single nucleotide polymorphisms (SNPs) have been identified in the beta(2)-adrenergic receptor gene (ADRB2). By the use of five SNPs (G46A, C79G, C491T, C523A, G1053C) for identification of ADRB2 haplotypes in 814 Danish Caucasians, we investigated whether ADRB2 haplotypes are associated with body mass index (BMI). The SNPs showed organization into 13 distinct haplotypes and 41 haplotype pairs. The study identified four common haplotypes: ACCCC (10.1 +/- 0.3 %), ACCCG (27.9 +/- 0.3 %), GCCAC (10.8 +/- 0.1 %) and GGCCG (41.0 +/- 0.2 %) (frequencies (SD), seen in 91 % of the population. In the total population (mean age +/- SD: 50 +/- 16 years), BMI was not related to haplotype pairs, individual SNPs or allelic haplotypes. However, in subjects < 50 years (N = 356, 36 +/- 8 years) BMI levels varied significantly between pairs of major haplotype groups (p = 0.014) but were not related to individual SNPs. In subjects < 37 years, the haplotype pair homozygote for the Gly16 and Glu27 amino acid variants (GGCCG/GGCCG) had a higher frequency of lean subjects (BMI < or = 25 kg/m(2)) compared with the GCCAC/GGCCG pair (73% versus 35%, odds ratio with 95% confidence interval: 4.95 (1.50-16.38). In conclusion, the haplotype analysis clearly revealed the prevalence of four major ADRB2 haplotypes in Caucasians. The results suggest that unique interactions in specific haplotype pairs rather than individual SNPs may affect BMI and that this effect of ADRB2 haplotypes is blunted by age-related factors.

Original language	English
Journal	Scandinavian Journal of Clinical & Laboratory Investigation
Volume	69
Issue number	7
Pages (from-to)	801-8
Number of pages	7
ISSN	0036-5513
DOIs	https://doi.org/10.3109/00365510903301536 https://doi.org/10.3109/00365510903301536
Publication status	Published - 2009

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Jensen, M. K., Nielsen, M., Koefoed, P., Nielsen, H. B., Ullum, H., Haastrup, E., Romner, B., Moltke, F. B., Olsen, N. V., Jensen, M. K., Nielsen, M., Koefoed, P., Nielsen, H. B., Ullum, H., Haastrup, E., Romner, B., Moltke, F. B., & Olsen, N. V. (2009). Haplotype structure of the beta2-adrenergic receptor gene in 814 Danish Caucasian subjects and association with body mass index. Scandinavian Journal of Clinical & Laboratory Investigation, 69(7), 801-8. https://doi.org/10.3109/00365510903301536, https://doi.org/10.3109/00365510903301536

Haplotype structure of the beta2-adrenergic receptor gene in 814 Danish Caucasian subjects and association with body mass index. / Jensen, Mette Kamp; Nielsen, Morten; Koefoed, Pernille et al.

In: Scandinavian Journal of Clinical & Laboratory Investigation, Vol. 69, No. 7, 2009, p. 801-8.

Research output: Contribution to journal › Journal article › Research › peer-review

Jensen, MK, Nielsen, M, Koefoed, P, Nielsen, HB, Ullum, H, Haastrup, E, Romner, B, Moltke, FB, Olsen, NV, Jensen, MK, Nielsen, M, Koefoed, P, Nielsen, HB, Ullum, H, Haastrup, E, Romner, B, Moltke, FB & Olsen, NV 2009, 'Haplotype structure of the beta2-adrenergic receptor gene in 814 Danish Caucasian subjects and association with body mass index', Scandinavian Journal of Clinical & Laboratory Investigation, vol. 69, no. 7, pp. 801-8. https://doi.org/10.3109/00365510903301536, https://doi.org/10.3109/00365510903301536

@article{713c3170529111df928f000ea68e967b,

title = "Haplotype structure of the beta2-adrenergic receptor gene in 814 Danish Caucasian subjects and association with body mass index",

abstract = "Several single nucleotide polymorphisms (SNPs) have been identified in the beta(2)-adrenergic receptor gene (ADRB2). By the use of five SNPs (G46A, C79G, C491T, C523A, G1053C) for identification of ADRB2 haplotypes in 814 Danish Caucasians, we investigated whether ADRB2 haplotypes are associated with body mass index (BMI). The SNPs showed organization into 13 distinct haplotypes and 41 haplotype pairs. The study identified four common haplotypes: ACCCC (10.1 +/- 0.3 %), ACCCG (27.9 +/- 0.3 %), GCCAC (10.8 +/- 0.1 %) and GGCCG (41.0 +/- 0.2 %) (frequencies (SD), seen in 91 % of the population. In the total population (mean age +/- SD: 50 +/- 16 years), BMI was not related to haplotype pairs, individual SNPs or allelic haplotypes. However, in subjects < 50 years (N = 356, 36 +/- 8 years) BMI levels varied significantly between pairs of major haplotype groups (p = 0.014) but were not related to individual SNPs. In subjects < 37 years, the haplotype pair homozygote for the Gly16 and Glu27 amino acid variants (GGCCG/GGCCG) had a higher frequency of lean subjects (BMI < or = 25 kg/m(2)) compared with the GCCAC/GGCCG pair (73% versus 35%, odds ratio with 95% confidence interval: 4.95 (1.50-16.38). In conclusion, the haplotype analysis clearly revealed the prevalence of four major ADRB2 haplotypes in Caucasians. The results suggest that unique interactions in specific haplotype pairs rather than individual SNPs may affect BMI and that this effect of ADRB2 haplotypes is blunted by age-related factors.",

author = "Jensen, {Mette Kamp} and Morten Nielsen and Pernille Koefoed and Nielsen, {Henning Bay} and Henrik Ullum and Eva Haastrup and Bertil Romner and Moltke, {Finn Borgbjerg} and Olsen, {Niels Vidiendal} and Jensen, {Mette Kamp} and Morten Nielsen and Pernille Koefoed and Nielsen, {Henning Bay} and Henrik Ullum and Eva Haastrup and Bertil Romner and Moltke, {Finn Borgbjerg} and Olsen, {Niels Vidiendal}",

note = "Keywords: Body Mass Index; Confidence Intervals; Denmark; European Continental Ancestry Group; Female; Haplotypes; Humans; Linkage Disequilibrium; Male; Middle Aged; Odds Ratio; Polymorphism, Single Nucleotide; Receptors, Adrenergic, beta-2",

year = "2009",

doi = "10.3109/00365510903301536",

language = "English",

volume = "69",

pages = "801--8",

journal = "Scandinavian Journal of Clinical & Laboratory Investigation",

issn = "0036-5513",

publisher = "Taylor & Francis",

number = "7",

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TY - JOUR

T1 - Haplotype structure of the beta2-adrenergic receptor gene in 814 Danish Caucasian subjects and association with body mass index

AU - Jensen, Mette Kamp

AU - Nielsen, Morten

AU - Koefoed, Pernille

AU - Nielsen, Henning Bay

AU - Ullum, Henrik

AU - Haastrup, Eva

AU - Romner, Bertil

AU - Moltke, Finn Borgbjerg

AU - Olsen, Niels Vidiendal

AU - Jensen, Mette Kamp

AU - Nielsen, Morten

AU - Koefoed, Pernille

AU - Nielsen, Henning Bay

AU - Ullum, Henrik

AU - Haastrup, Eva

AU - Romner, Bertil

AU - Moltke, Finn Borgbjerg

AU - Olsen, Niels Vidiendal

N1 - Keywords: Body Mass Index; Confidence Intervals; Denmark; European Continental Ancestry Group; Female; Haplotypes; Humans; Linkage Disequilibrium; Male; Middle Aged; Odds Ratio; Polymorphism, Single Nucleotide; Receptors, Adrenergic, beta-2

PY - 2009

Y1 - 2009

N2 - Several single nucleotide polymorphisms (SNPs) have been identified in the beta(2)-adrenergic receptor gene (ADRB2). By the use of five SNPs (G46A, C79G, C491T, C523A, G1053C) for identification of ADRB2 haplotypes in 814 Danish Caucasians, we investigated whether ADRB2 haplotypes are associated with body mass index (BMI). The SNPs showed organization into 13 distinct haplotypes and 41 haplotype pairs. The study identified four common haplotypes: ACCCC (10.1 +/- 0.3 %), ACCCG (27.9 +/- 0.3 %), GCCAC (10.8 +/- 0.1 %) and GGCCG (41.0 +/- 0.2 %) (frequencies (SD), seen in 91 % of the population. In the total population (mean age +/- SD: 50 +/- 16 years), BMI was not related to haplotype pairs, individual SNPs or allelic haplotypes. However, in subjects < 50 years (N = 356, 36 +/- 8 years) BMI levels varied significantly between pairs of major haplotype groups (p = 0.014) but were not related to individual SNPs. In subjects < 37 years, the haplotype pair homozygote for the Gly16 and Glu27 amino acid variants (GGCCG/GGCCG) had a higher frequency of lean subjects (BMI < or = 25 kg/m(2)) compared with the GCCAC/GGCCG pair (73% versus 35%, odds ratio with 95% confidence interval: 4.95 (1.50-16.38). In conclusion, the haplotype analysis clearly revealed the prevalence of four major ADRB2 haplotypes in Caucasians. The results suggest that unique interactions in specific haplotype pairs rather than individual SNPs may affect BMI and that this effect of ADRB2 haplotypes is blunted by age-related factors.

AB - Several single nucleotide polymorphisms (SNPs) have been identified in the beta(2)-adrenergic receptor gene (ADRB2). By the use of five SNPs (G46A, C79G, C491T, C523A, G1053C) for identification of ADRB2 haplotypes in 814 Danish Caucasians, we investigated whether ADRB2 haplotypes are associated with body mass index (BMI). The SNPs showed organization into 13 distinct haplotypes and 41 haplotype pairs. The study identified four common haplotypes: ACCCC (10.1 +/- 0.3 %), ACCCG (27.9 +/- 0.3 %), GCCAC (10.8 +/- 0.1 %) and GGCCG (41.0 +/- 0.2 %) (frequencies (SD), seen in 91 % of the population. In the total population (mean age +/- SD: 50 +/- 16 years), BMI was not related to haplotype pairs, individual SNPs or allelic haplotypes. However, in subjects < 50 years (N = 356, 36 +/- 8 years) BMI levels varied significantly between pairs of major haplotype groups (p = 0.014) but were not related to individual SNPs. In subjects < 37 years, the haplotype pair homozygote for the Gly16 and Glu27 amino acid variants (GGCCG/GGCCG) had a higher frequency of lean subjects (BMI < or = 25 kg/m(2)) compared with the GCCAC/GGCCG pair (73% versus 35%, odds ratio with 95% confidence interval: 4.95 (1.50-16.38). In conclusion, the haplotype analysis clearly revealed the prevalence of four major ADRB2 haplotypes in Caucasians. The results suggest that unique interactions in specific haplotype pairs rather than individual SNPs may affect BMI and that this effect of ADRB2 haplotypes is blunted by age-related factors.

U2 - 10.3109/00365510903301536

DO - 10.3109/00365510903301536

M3 - Journal article

C2 - 19929724

SN - 0036-5513

VL - 69

SP - 801

EP - 808

JO - Scandinavian Journal of Clinical & Laboratory Investigation

JF - Scandinavian Journal of Clinical & Laboratory Investigation

IS - 7

ER -

Haplotype structure of the beta2-adrenergic receptor gene in 814 Danish Caucasian subjects and association with body mass index

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