Growth-rate-dependent transcription initiation from the dam P2 promoter

L J Rasmussen; A Løbner-Olesen; M G Marinus

Growth-rate-dependent transcription initiation from the dam P2 promoter

L J Rasmussen, A Løbner-Olesen, M G Marinus

17 Citations (Scopus)

Abstract

Transcription of the dam gene in Escherichia coli is dependent on growth rate. Using single-copy promoter::lacZYA fusions we found that of the five promoter regions which affect dam expression, only the P2 promoter shows growth-rate dependence. The determinants for growth-rate control must lie in the region -52 to +27 relative to the transcription start point.

Original language	English
Journal	Gene
Volume	157
Issue number	1-2
Pages (from-to)	213-5
Number of pages	3
ISSN	0378-1119
Publication status	Published - 19 May 1995

Keywords

Base Sequence
Colon/microbiology
Escherichia coli/genetics
Escherichia coli Proteins
Gene Expression Regulation, Bacterial
Humans
Methyltransferases/biosynthesis
Promoter Regions, Genetic
Recombinant Fusion Proteins/biosynthesis
Site-Specific DNA-Methyltransferase (Adenine-Specific)
Substrate Specificity
beta-Galactosidase/biosynthesis

Cite this

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title = "Growth-rate-dependent transcription initiation from the dam P2 promoter",

abstract = "Transcription of the dam gene in Escherichia coli is dependent on growth rate. Using single-copy promoter::lacZYA fusions we found that of the five promoter regions which affect dam expression, only the P2 promoter shows growth-rate dependence. The determinants for growth-rate control must lie in the region -52 to +27 relative to the transcription start point.",

keywords = "Base Sequence, Colon/microbiology, Escherichia coli/genetics, Escherichia coli Proteins, Gene Expression Regulation, Bacterial, Humans, Methyltransferases/biosynthesis, Promoter Regions, Genetic, Recombinant Fusion Proteins/biosynthesis, Site-Specific DNA-Methyltransferase (Adenine-Specific), Substrate Specificity, beta-Galactosidase/biosynthesis",

author = "Rasmussen, {L J} and A L{\o}bner-Olesen and Marinus, {M G}",

year = "1995",

month = may,

day = "19",

language = "English",

volume = "157",

pages = "213--5",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier",

number = "1-2",

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TY - JOUR

T1 - Growth-rate-dependent transcription initiation from the dam P2 promoter

AU - Rasmussen, L J

AU - Løbner-Olesen, A

AU - Marinus, M G

PY - 1995/5/19

Y1 - 1995/5/19

N2 - Transcription of the dam gene in Escherichia coli is dependent on growth rate. Using single-copy promoter::lacZYA fusions we found that of the five promoter regions which affect dam expression, only the P2 promoter shows growth-rate dependence. The determinants for growth-rate control must lie in the region -52 to +27 relative to the transcription start point.

AB - Transcription of the dam gene in Escherichia coli is dependent on growth rate. Using single-copy promoter::lacZYA fusions we found that of the five promoter regions which affect dam expression, only the P2 promoter shows growth-rate dependence. The determinants for growth-rate control must lie in the region -52 to +27 relative to the transcription start point.

KW - Base Sequence

KW - Colon/microbiology

KW - Escherichia coli/genetics

KW - Escherichia coli Proteins

KW - Gene Expression Regulation, Bacterial

KW - Humans

KW - Methyltransferases/biosynthesis

KW - Promoter Regions, Genetic

KW - Recombinant Fusion Proteins/biosynthesis

KW - Site-Specific DNA-Methyltransferase (Adenine-Specific)

KW - Substrate Specificity

KW - beta-Galactosidase/biosynthesis

M3 - Journal article

C2 - 7607492

SN - 0378-1119

VL - 157

SP - 213

EP - 215

JO - Gene

JF - Gene

IS - 1-2

ER -

Growth-rate-dependent transcription initiation from the dam P2 promoter

Abstract

Keywords

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