Growth hormone and prolactin stimulate the expression of rat preadipocyte factor-1/delta-like protein in pancreatic islets: molecular cloning and expression pattern during development and growth of the endocrine pancreas

C Carlsson; D Tornehave; Karen Lindberg; P Galante; N Billestrup; Birgitte Michelsen; L I Larsson; Jens Høiriis Nielsen

Growth hormone and prolactin stimulate the expression of rat preadipocyte factor-1/delta-like protein in pancreatic islets: molecular cloning and expression pattern during development and growth of the endocrine pancreas

C Carlsson, D Tornehave, Karen Lindberg, P Galante, N Billestrup, Birgitte Michelsen, L I Larsson, Jens Høiriis Nielsen

Inflammation, Metabolism and Oxidation

89 Citations (Scopus)

Abstract

GH and PRL have been shown to stimulate proliferation and insulin production in islets of Langerhans. To identify genes regulated by GH/PRL in islets, we performed differential screening of a complementary DNA library from neonatal rat islets cultured for 24 h with human GH (hGH). One hGH-induced clone had 96% identity with mouse preadipocyte factor-1 (Pref-1, or delta-like protein (Dlk)]. The size of Pref-1 messenger RNA (mRNA) in islets was 1.6 kilobases, with two less abundant mRNAs of 3.7 and 6.2 kilobases. The Pref-1 mRNA content of islets from adult rats was only 1% of that in neonatal islets. Pref-1 mRNA was markedly up-regulated in islets from pregnant rats from day 12 to term compared with those from age-matched female rats. Two peaks in mRNA expression were observed during gestation, one on day 14 and the other at term, whereafter it decreased to nonpregnant levels. Pref-1 mRNA was up-regulated 3- to 4-fold in neonatal rat islets of Langerhans after 48-h culture with hGH, as found also with bovine GH or ovine PRL. During the development of pancreas from embryonic day 12 (E12) to postnatal day 4, we observed a 2-fold increase in Pref-1 mRNA on E17 and a 5-fold increase at birth, followed by a rapid decline on postnatal day 4. Pref-1 immunoreactivity was found in a subpopulation of insulin cells of neonatal islets of Langerhans. At an early embryonal stage (E13), most cells of the pancreatic anlage were Pref-1 positive, becoming predominantly restricted to the insulin-producing cells during development. In conclusion, these findings suggest that Pref-1 is involved in both differentiation and growth of beta-cells.

Original language	English
Journal	Endocrinology
Volume	138
Issue number	9
Pages (from-to)	3940-8
Number of pages	9
ISSN	0013-7227
Publication status	Published - Sept 1997

Keywords

Amino Acid Sequence
Animals
Animals, Newborn
Cloning, Molecular
Female
Gene Expression
Growth Hormone
Humans
Intercellular Signaling Peptides and Proteins
Islets of Langerhans
Male
Membrane Proteins
Mice
Molecular Sequence Data
Polymerase Chain Reaction
Pregnancy
Prolactin
RNA, Messenger
Rats
Rats, Wistar
Repressor Proteins
Sequence Alignment

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Carlsson, C., Tornehave, D., Lindberg, K., Galante, P., Billestrup, N., Michelsen, B., Larsson, L. I., & Nielsen, J. H. (1997). Growth hormone and prolactin stimulate the expression of rat preadipocyte factor-1/delta-like protein in pancreatic islets: molecular cloning and expression pattern during development and growth of the endocrine pancreas. Endocrinology, 138(9), 3940-8.

Growth hormone and prolactin stimulate the expression of rat preadipocyte factor-1/delta-like protein in pancreatic islets: molecular cloning and expression pattern during development and growth of the endocrine pancreas. / Carlsson, C; Tornehave, D; Lindberg, Karen et al.
In: Endocrinology, Vol. 138, No. 9, 09.1997, p. 3940-8.

Research output: Contribution to journal › Journal article › Research › peer-review

Carlsson, C, Tornehave, D, Lindberg, K, Galante, P, Billestrup, N, Michelsen, B, Larsson, LI & Nielsen, JH 1997, 'Growth hormone and prolactin stimulate the expression of rat preadipocyte factor-1/delta-like protein in pancreatic islets: molecular cloning and expression pattern during development and growth of the endocrine pancreas', Endocrinology, vol. 138, no. 9, pp. 3940-8.

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title = "Growth hormone and prolactin stimulate the expression of rat preadipocyte factor-1/delta-like protein in pancreatic islets: molecular cloning and expression pattern during development and growth of the endocrine pancreas",

abstract = "GH and PRL have been shown to stimulate proliferation and insulin production in islets of Langerhans. To identify genes regulated by GH/PRL in islets, we performed differential screening of a complementary DNA library from neonatal rat islets cultured for 24 h with human GH (hGH). One hGH-induced clone had 96% identity with mouse preadipocyte factor-1 (Pref-1, or delta-like protein (Dlk)]. The size of Pref-1 messenger RNA (mRNA) in islets was 1.6 kilobases, with two less abundant mRNAs of 3.7 and 6.2 kilobases. The Pref-1 mRNA content of islets from adult rats was only 1% of that in neonatal islets. Pref-1 mRNA was markedly up-regulated in islets from pregnant rats from day 12 to term compared with those from age-matched female rats. Two peaks in mRNA expression were observed during gestation, one on day 14 and the other at term, whereafter it decreased to nonpregnant levels. Pref-1 mRNA was up-regulated 3- to 4-fold in neonatal rat islets of Langerhans after 48-h culture with hGH, as found also with bovine GH or ovine PRL. During the development of pancreas from embryonic day 12 (E12) to postnatal day 4, we observed a 2-fold increase in Pref-1 mRNA on E17 and a 5-fold increase at birth, followed by a rapid decline on postnatal day 4. Pref-1 immunoreactivity was found in a subpopulation of insulin cells of neonatal islets of Langerhans. At an early embryonal stage (E13), most cells of the pancreatic anlage were Pref-1 positive, becoming predominantly restricted to the insulin-producing cells during development. In conclusion, these findings suggest that Pref-1 is involved in both differentiation and growth of beta-cells.",

keywords = "Amino Acid Sequence, Animals, Animals, Newborn, Cloning, Molecular, Female, Gene Expression, Growth Hormone, Humans, Intercellular Signaling Peptides and Proteins, Islets of Langerhans, Male, Membrane Proteins, Mice, Molecular Sequence Data, Polymerase Chain Reaction, Pregnancy, Prolactin, RNA, Messenger, Rats, Rats, Wistar, Repressor Proteins, Sequence Alignment",

author = "C Carlsson and D Tornehave and Karen Lindberg and P Galante and N Billestrup and Birgitte Michelsen and Larsson, {L I} and Nielsen, {Jens H{\o}iriis}",

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T1 - Growth hormone and prolactin stimulate the expression of rat preadipocyte factor-1/delta-like protein in pancreatic islets

T2 - molecular cloning and expression pattern during development and growth of the endocrine pancreas

AU - Carlsson, C

AU - Tornehave, D

AU - Lindberg, Karen

AU - Galante, P

AU - Billestrup, N

AU - Michelsen, Birgitte

AU - Larsson, L I

AU - Nielsen, Jens Høiriis

PY - 1997/9

Y1 - 1997/9

N2 - GH and PRL have been shown to stimulate proliferation and insulin production in islets of Langerhans. To identify genes regulated by GH/PRL in islets, we performed differential screening of a complementary DNA library from neonatal rat islets cultured for 24 h with human GH (hGH). One hGH-induced clone had 96% identity with mouse preadipocyte factor-1 (Pref-1, or delta-like protein (Dlk)]. The size of Pref-1 messenger RNA (mRNA) in islets was 1.6 kilobases, with two less abundant mRNAs of 3.7 and 6.2 kilobases. The Pref-1 mRNA content of islets from adult rats was only 1% of that in neonatal islets. Pref-1 mRNA was markedly up-regulated in islets from pregnant rats from day 12 to term compared with those from age-matched female rats. Two peaks in mRNA expression were observed during gestation, one on day 14 and the other at term, whereafter it decreased to nonpregnant levels. Pref-1 mRNA was up-regulated 3- to 4-fold in neonatal rat islets of Langerhans after 48-h culture with hGH, as found also with bovine GH or ovine PRL. During the development of pancreas from embryonic day 12 (E12) to postnatal day 4, we observed a 2-fold increase in Pref-1 mRNA on E17 and a 5-fold increase at birth, followed by a rapid decline on postnatal day 4. Pref-1 immunoreactivity was found in a subpopulation of insulin cells of neonatal islets of Langerhans. At an early embryonal stage (E13), most cells of the pancreatic anlage were Pref-1 positive, becoming predominantly restricted to the insulin-producing cells during development. In conclusion, these findings suggest that Pref-1 is involved in both differentiation and growth of beta-cells.

AB - GH and PRL have been shown to stimulate proliferation and insulin production in islets of Langerhans. To identify genes regulated by GH/PRL in islets, we performed differential screening of a complementary DNA library from neonatal rat islets cultured for 24 h with human GH (hGH). One hGH-induced clone had 96% identity with mouse preadipocyte factor-1 (Pref-1, or delta-like protein (Dlk)]. The size of Pref-1 messenger RNA (mRNA) in islets was 1.6 kilobases, with two less abundant mRNAs of 3.7 and 6.2 kilobases. The Pref-1 mRNA content of islets from adult rats was only 1% of that in neonatal islets. Pref-1 mRNA was markedly up-regulated in islets from pregnant rats from day 12 to term compared with those from age-matched female rats. Two peaks in mRNA expression were observed during gestation, one on day 14 and the other at term, whereafter it decreased to nonpregnant levels. Pref-1 mRNA was up-regulated 3- to 4-fold in neonatal rat islets of Langerhans after 48-h culture with hGH, as found also with bovine GH or ovine PRL. During the development of pancreas from embryonic day 12 (E12) to postnatal day 4, we observed a 2-fold increase in Pref-1 mRNA on E17 and a 5-fold increase at birth, followed by a rapid decline on postnatal day 4. Pref-1 immunoreactivity was found in a subpopulation of insulin cells of neonatal islets of Langerhans. At an early embryonal stage (E13), most cells of the pancreatic anlage were Pref-1 positive, becoming predominantly restricted to the insulin-producing cells during development. In conclusion, these findings suggest that Pref-1 is involved in both differentiation and growth of beta-cells.

KW - Amino Acid Sequence

KW - Animals

KW - Animals, Newborn

KW - Cloning, Molecular

KW - Female

KW - Gene Expression

KW - Growth Hormone

KW - Humans

KW - Intercellular Signaling Peptides and Proteins

KW - Islets of Langerhans

KW - Male

KW - Membrane Proteins

KW - Mice

KW - Molecular Sequence Data

KW - Polymerase Chain Reaction

KW - Pregnancy

KW - Prolactin

KW - RNA, Messenger

KW - Rats

KW - Rats, Wistar

KW - Repressor Proteins

KW - Sequence Alignment

M3 - Journal article

C2 - 9275085

SN - 0013-7227

VL - 138

SP - 3940

EP - 3948

JO - Endocrinology

JF - Endocrinology

IS - 9

ER -