Gold-embedded photosensitive liposomes for drug delivery: Triggering mechanism and intracellular release

TY - JOUR

T1 - Gold-embedded photosensitive liposomes for drug delivery: Triggering mechanism and intracellular release

AU - Paasonen, Lauri

AU - Sipilä, Tuomas

AU - Subrizi, Astrid

AU - Laurinmäki, Pasi

AU - Butcher, Sarah J.

AU - Rappolt, Michael

AU - Yaghmur, Anan

AU - Urtti, Arto

AU - Yliperttula, Marjo

PY - 2010/10

Y1 - 2010/10

N2 - Liposomes embedded with gold nanoparticles show light-triggered contents release. We investigated the mechanism of the light-induced changes and functionality of the light-induced release in the cells. The real time small angle X-ray scattering (SAXS) analysis revealed time-dependent phase transitions in distearoylphosphatidylcholine (DSPC)/dipalmitoylphosphatidylcholine (DPPC) liposomes upon heating. Similar changes were observed when gold nanoparticle-embedded liposomes were exposed to the UV light: gold nanoparticles absorb light energy and transfer it to heat, thereby causing lipid phase transition from gel phase to rippled phase, and further to fluid phase. Without UV light exposure the gold nanoparticles did not affect the liposomal bilayer periodicity. The light-triggered release of hydrophilic fluorescent probe (calcein) from the gold nanoparticle-loaded liposomes was demonstrated with fluorescence-activated cell sorting after liposome internalization into the ARPE-19 cells. The liposome formulations did not decrease the cell viability in vitro. In conclusion, the light-triggered release from the liposomes is functional in the cells, and the release is triggered by thermal phase changes in the lipid bilayers.

AB - Liposomes embedded with gold nanoparticles show light-triggered contents release. We investigated the mechanism of the light-induced changes and functionality of the light-induced release in the cells. The real time small angle X-ray scattering (SAXS) analysis revealed time-dependent phase transitions in distearoylphosphatidylcholine (DSPC)/dipalmitoylphosphatidylcholine (DPPC) liposomes upon heating. Similar changes were observed when gold nanoparticle-embedded liposomes were exposed to the UV light: gold nanoparticles absorb light energy and transfer it to heat, thereby causing lipid phase transition from gel phase to rippled phase, and further to fluid phase. Without UV light exposure the gold nanoparticles did not affect the liposomal bilayer periodicity. The light-triggered release of hydrophilic fluorescent probe (calcein) from the gold nanoparticle-loaded liposomes was demonstrated with fluorescence-activated cell sorting after liposome internalization into the ARPE-19 cells. The liposome formulations did not decrease the cell viability in vitro. In conclusion, the light-triggered release from the liposomes is functional in the cells, and the release is triggered by thermal phase changes in the lipid bilayers.

KW - Former Faculty of Pharmaceutical Sciences

U2 - 10.1016/j.conrel.2010.07.095

DO - 10.1016/j.conrel.2010.07.095

M3 - Journal article

SN - 0168-3659

VL - 147

SP - 136

EP - 143

JO - Journal of Controlled Release

JF - Journal of Controlled Release

ER -