Glyphosate accumulation, translocation, and biological effects in Coffea arabica after single and multiple exposures

Lars Christoph Schrübbers; Bernal E. Valverde; Bjarne W. Strobel; Nina Cedergreen

doi:10.1016/j.eja.2015.11.023

Glyphosate accumulation, translocation, and biological effects in Coffea arabica after single and multiple exposures

Lars Christoph Schrübbers, Bernal E. Valverde, Bjarne W. Strobel, Nina Cedergreen

Section for Environmental Chemistry and Physics

6 Citations (Scopus)

Abstract

In perennial crops like coffee, glyphosate drift exposure can occur multiple times during its commercial life span. Due to limited glyphosate degradation in higher plants, a potential accumulation of glyphosate could lead to increased biological effects with increased exposure frequency. In this study, we investigated glyphosate translocation over time, and its concentration and biological effects after single and multiple simulated spray-drift exposures. Additionally, shikimic acid/glyphosate ratios were used as biomarkers for glyphosate binding to its target enzyme.Four weeks after the exposure, glyphosate was continuously translocated. Shikimic acid levels were lin-ear correlated with glyphosate levels. After two months, however, glyphosate appeared to have reduced activity. In the greenhouse, multiple applications resulted in higher internal glyphosate concentrations.The time of application, however, was more important regarding biological effects than the number of applications both in the greenhouse and in the field. In the field, berry yield, the most important biological response variable, was reduced 26% by the first out of four sequential applications of glyphosate at 64 g a.e. ha⁻ 1each. The three subsequent applications did not reduce yield any further.

Original language	English
Journal	European Journal of Agronomy
Volume	74
Pages (from-to)	133-143
Number of pages	11
ISSN	1161-0301
DOIs	https://doi.org/10.1016/j.eja.2015.11.023
Publication status	Published - 1 Mar 2016

Access to Document

10.1016/j.eja.2015.11.023

Cite this

@article{662966b783a241f3b67db74a0bbbb4f5,

title = "Glyphosate accumulation, translocation, and biological effects in Coffea arabica after single and multiple exposures",

abstract = "In perennial crops like coffee, glyphosate drift exposure can occur multiple times during its commercial life span. Due to limited glyphosate degradation in higher plants, a potential accumulation of glyphosate could lead to increased biological effects with increased exposure frequency. In this study, we investigated glyphosate translocation over time, and its concentration and biological effects after single and multiple simulated spray-drift exposures. Additionally, shikimic acid/glyphosate ratios were used as biomarkers for glyphosate binding to its target enzyme.Four weeks after the exposure, glyphosate was continuously translocated. Shikimic acid levels were lin-ear correlated with glyphosate levels. After two months, however, glyphosate appeared to have reduced activity. In the greenhouse, multiple applications resulted in higher internal glyphosate concentrations.The time of application, however, was more important regarding biological effects than the number of applications both in the greenhouse and in the field. In the field, berry yield, the most important biological response variable, was reduced 26% by the first out of four sequential applications of glyphosate at 64 g a.e. ha− 1each. The three subsequent applications did not reduce yield any further.",

author = "Schr{\"u}bbers, {Lars Christoph} and Valverde, {Bernal E.} and Strobel, {Bjarne W.} and Nina Cedergreen",

year = "2016",

month = mar,

day = "1",

doi = "10.1016/j.eja.2015.11.023",

language = "English",

volume = "74",

pages = "133--143",

journal = "European Journal of Agronomy",

issn = "1161-0301",

publisher = "Elsevier",

}

TY - JOUR

T1 - Glyphosate accumulation, translocation, and biological effects in Coffea arabica after single and multiple exposures

AU - Schrübbers, Lars Christoph

AU - Valverde, Bernal E.

AU - Strobel, Bjarne W.

AU - Cedergreen, Nina

PY - 2016/3/1

Y1 - 2016/3/1

N2 - In perennial crops like coffee, glyphosate drift exposure can occur multiple times during its commercial life span. Due to limited glyphosate degradation in higher plants, a potential accumulation of glyphosate could lead to increased biological effects with increased exposure frequency. In this study, we investigated glyphosate translocation over time, and its concentration and biological effects after single and multiple simulated spray-drift exposures. Additionally, shikimic acid/glyphosate ratios were used as biomarkers for glyphosate binding to its target enzyme.Four weeks after the exposure, glyphosate was continuously translocated. Shikimic acid levels were lin-ear correlated with glyphosate levels. After two months, however, glyphosate appeared to have reduced activity. In the greenhouse, multiple applications resulted in higher internal glyphosate concentrations.The time of application, however, was more important regarding biological effects than the number of applications both in the greenhouse and in the field. In the field, berry yield, the most important biological response variable, was reduced 26% by the first out of four sequential applications of glyphosate at 64 g a.e. ha− 1each. The three subsequent applications did not reduce yield any further.

AB - In perennial crops like coffee, glyphosate drift exposure can occur multiple times during its commercial life span. Due to limited glyphosate degradation in higher plants, a potential accumulation of glyphosate could lead to increased biological effects with increased exposure frequency. In this study, we investigated glyphosate translocation over time, and its concentration and biological effects after single and multiple simulated spray-drift exposures. Additionally, shikimic acid/glyphosate ratios were used as biomarkers for glyphosate binding to its target enzyme.Four weeks after the exposure, glyphosate was continuously translocated. Shikimic acid levels were lin-ear correlated with glyphosate levels. After two months, however, glyphosate appeared to have reduced activity. In the greenhouse, multiple applications resulted in higher internal glyphosate concentrations.The time of application, however, was more important regarding biological effects than the number of applications both in the greenhouse and in the field. In the field, berry yield, the most important biological response variable, was reduced 26% by the first out of four sequential applications of glyphosate at 64 g a.e. ha− 1each. The three subsequent applications did not reduce yield any further.

U2 - 10.1016/j.eja.2015.11.023

DO - 10.1016/j.eja.2015.11.023

M3 - Journal article

SN - 1161-0301

VL - 74

SP - 133

EP - 143

JO - European Journal of Agronomy

JF - European Journal of Agronomy

ER -