Glucose tolerance is associated with differential expression of microRNAs in skeletal muscle: results from studies of twins with and without type 2 diabetes

Jette Bork-Jensen; Camilla Charlotte Schéele; Daniel V Christophersen; Emma Nilsson; Martin Friedrichsen; Denise S Fernandez-Twinn; Louise G Grunnet; Thomas Litman; Kim Holmstrøm; Birgitte Vind; Kurt Højlund; Henning Beck-Nielsen; Jørgen Wojtaszewski; Susan E Ozanne; Bente K Pedersen; Pernille Poulsen; Allan Vaag

doi:10.1007/s00125-014-3434-2

Glucose tolerance is associated with differential expression of microRNAs in skeletal muscle: results from studies of twins with and without type 2 diabetes

Jette Bork-Jensen, Camilla Charlotte Schéele, Daniel V Christophersen, Emma Nilsson, Martin Friedrichsen, Denise S Fernandez-Twinn, Louise G Grunnet, Thomas Litman, Kim Holmstrøm, Birgitte Vind, Kurt Højlund, Henning Beck-Nielsen, Jørgen Wojtaszewski, Susan E Ozanne, Bente K Pedersen, Pernille Poulsen, Allan Vaag

40 Citations (Scopus)

Abstract

Methods: We measured muscle miRNA levels in monozygotic (MZ) twins discordant for type 2 diabetes using arrays. Further investigations of selected miRNAs included target prediction, pathway analysis, silencing in cells and association analyses in a separate cohort of 164 non-diabetic MZ and dizygotic twins. The effects of elevated glucose and insulin levels on miRNA expression were examined, and the effect of low birthweight (LBW) was studied in rats.

Aims/hypothesis: We aimed to identify microRNAs (miRNAs) associated with type 2 diabetes and risk of developing the disease in skeletal muscle biopsies from phenotypically well-characterised twins.

Conclusions: Type 2 diabetes is associated with non-genetic downregulation of several miRNAs in skeletal muscle including miR-15b and miR-16, potentially targeting insulin signalling. The paradoxical findings in twins with overt diabetes and twins at increased risk of the disease underscore the complexity of the regulation of muscle insulin signalling in glucose homeostasis.

Results: We identified 20 miRNAs that were downregulated in MZ twins with diabetes compared with their non-diabetic co-twins. Differences for members of the miR-15 family (miR-15b and miR-16) were the most statistically significant, and these miRNAs were predicted to influence insulin signalling. Indeed, miR-15b and miR-16 levels were associated with levels of key insulin signalling proteins, miR-15b was associated with the insulin receptor in non-diabetic twins and knockdown of miR-15b/miR-16 in myocytes changed the levels of insulin signalling proteins. LBW in twins and undernutrition during pregnancy in rats were, in contrast to overt type 2 diabetes, associated with increased expression of miR-15b and/or miR-16. Elevated glucose and insulin suppressed miR-16 expression in vitro.

Original language	English
Journal	Diabetologia
Volume	58
Issue number	2
Pages (from-to)	363-373
Number of pages	11
ISSN	0012-186X
DOIs	https://doi.org/10.1007/s00125-014-3434-2
Publication status	Published - Feb 2015

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Bork-Jensen, J., Schéele, C. C., Christophersen, D. V., Nilsson, E., Friedrichsen, M., Fernandez-Twinn, D. S., Grunnet, L. G., Litman, T., Holmstrøm, K., Vind, B., Højlund, K., Beck-Nielsen, H., Wojtaszewski, J., Ozanne, S. E., Pedersen, B. K., Poulsen, P., & Vaag, A. (2015). Glucose tolerance is associated with differential expression of microRNAs in skeletal muscle: results from studies of twins with and without type 2 diabetes. Diabetologia, 58(2), 363-373. https://doi.org/10.1007/s00125-014-3434-2

Glucose tolerance is associated with differential expression of microRNAs in skeletal muscle: results from studies of twins with and without type 2 diabetes. / Bork-Jensen, Jette; Schéele, Camilla Charlotte; Christophersen, Daniel V et al.
In: Diabetologia, Vol. 58, No. 2, 02.2015, p. 363-373.

Research output: Contribution to journal › Journal article › Research › peer-review

Bork-Jensen, J, Schéele, CC, Christophersen, DV, Nilsson, E, Friedrichsen, M, Fernandez-Twinn, DS, Grunnet, LG, Litman, T, Holmstrøm, K, Vind, B, Højlund, K, Beck-Nielsen, H, Wojtaszewski, J, Ozanne, SE, Pedersen, BK, Poulsen, P & Vaag, A 2015, 'Glucose tolerance is associated with differential expression of microRNAs in skeletal muscle: results from studies of twins with and without type 2 diabetes', Diabetologia, vol. 58, no. 2, pp. 363-373. https://doi.org/10.1007/s00125-014-3434-2

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title = "Glucose tolerance is associated with differential expression of microRNAs in skeletal muscle: results from studies of twins with and without type 2 diabetes",

abstract = "Methods: We measured muscle miRNA levels in monozygotic (MZ) twins discordant for type 2 diabetes using arrays. Further investigations of selected miRNAs included target prediction, pathway analysis, silencing in cells and association analyses in a separate cohort of 164 non-diabetic MZ and dizygotic twins. The effects of elevated glucose and insulin levels on miRNA expression were examined, and the effect of low birthweight (LBW) was studied in rats.Aims/hypothesis: We aimed to identify microRNAs (miRNAs) associated with type 2 diabetes and risk of developing the disease in skeletal muscle biopsies from phenotypically well-characterised twins.Conclusions: Type 2 diabetes is associated with non-genetic downregulation of several miRNAs in skeletal muscle including miR-15b and miR-16, potentially targeting insulin signalling. The paradoxical findings in twins with overt diabetes and twins at increased risk of the disease underscore the complexity of the regulation of muscle insulin signalling in glucose homeostasis.Results: We identified 20 miRNAs that were downregulated in MZ twins with diabetes compared with their non-diabetic co-twins. Differences for members of the miR-15 family (miR-15b and miR-16) were the most statistically significant, and these miRNAs were predicted to influence insulin signalling. Indeed, miR-15b and miR-16 levels were associated with levels of key insulin signalling proteins, miR-15b was associated with the insulin receptor in non-diabetic twins and knockdown of miR-15b/miR-16 in myocytes changed the levels of insulin signalling proteins. LBW in twins and undernutrition during pregnancy in rats were, in contrast to overt type 2 diabetes, associated with increased expression of miR-15b and/or miR-16. Elevated glucose and insulin suppressed miR-16 expression in vitro.",

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T1 - Glucose tolerance is associated with differential expression of microRNAs in skeletal muscle

T2 - results from studies of twins with and without type 2 diabetes

AU - Bork-Jensen, Jette

AU - Schéele, Camilla Charlotte

AU - Christophersen, Daniel V

AU - Nilsson, Emma

AU - Friedrichsen, Martin

AU - Fernandez-Twinn, Denise S

AU - Grunnet, Louise G

AU - Litman, Thomas

AU - Holmstrøm, Kim

AU - Vind, Birgitte

AU - Højlund, Kurt

AU - Beck-Nielsen, Henning

AU - Wojtaszewski, Jørgen

AU - Ozanne, Susan E

AU - Pedersen, Bente K

AU - Poulsen, Pernille

AU - Vaag, Allan

N1 - CURIS 2015 NEXS 024

PY - 2015/2

Y1 - 2015/2

N2 - Methods: We measured muscle miRNA levels in monozygotic (MZ) twins discordant for type 2 diabetes using arrays. Further investigations of selected miRNAs included target prediction, pathway analysis, silencing in cells and association analyses in a separate cohort of 164 non-diabetic MZ and dizygotic twins. The effects of elevated glucose and insulin levels on miRNA expression were examined, and the effect of low birthweight (LBW) was studied in rats.Aims/hypothesis: We aimed to identify microRNAs (miRNAs) associated with type 2 diabetes and risk of developing the disease in skeletal muscle biopsies from phenotypically well-characterised twins.Conclusions: Type 2 diabetes is associated with non-genetic downregulation of several miRNAs in skeletal muscle including miR-15b and miR-16, potentially targeting insulin signalling. The paradoxical findings in twins with overt diabetes and twins at increased risk of the disease underscore the complexity of the regulation of muscle insulin signalling in glucose homeostasis.Results: We identified 20 miRNAs that were downregulated in MZ twins with diabetes compared with their non-diabetic co-twins. Differences for members of the miR-15 family (miR-15b and miR-16) were the most statistically significant, and these miRNAs were predicted to influence insulin signalling. Indeed, miR-15b and miR-16 levels were associated with levels of key insulin signalling proteins, miR-15b was associated with the insulin receptor in non-diabetic twins and knockdown of miR-15b/miR-16 in myocytes changed the levels of insulin signalling proteins. LBW in twins and undernutrition during pregnancy in rats were, in contrast to overt type 2 diabetes, associated with increased expression of miR-15b and/or miR-16. Elevated glucose and insulin suppressed miR-16 expression in vitro.

AB - Methods: We measured muscle miRNA levels in monozygotic (MZ) twins discordant for type 2 diabetes using arrays. Further investigations of selected miRNAs included target prediction, pathway analysis, silencing in cells and association analyses in a separate cohort of 164 non-diabetic MZ and dizygotic twins. The effects of elevated glucose and insulin levels on miRNA expression were examined, and the effect of low birthweight (LBW) was studied in rats.Aims/hypothesis: We aimed to identify microRNAs (miRNAs) associated with type 2 diabetes and risk of developing the disease in skeletal muscle biopsies from phenotypically well-characterised twins.Conclusions: Type 2 diabetes is associated with non-genetic downregulation of several miRNAs in skeletal muscle including miR-15b and miR-16, potentially targeting insulin signalling. The paradoxical findings in twins with overt diabetes and twins at increased risk of the disease underscore the complexity of the regulation of muscle insulin signalling in glucose homeostasis.Results: We identified 20 miRNAs that were downregulated in MZ twins with diabetes compared with their non-diabetic co-twins. Differences for members of the miR-15 family (miR-15b and miR-16) were the most statistically significant, and these miRNAs were predicted to influence insulin signalling. Indeed, miR-15b and miR-16 levels were associated with levels of key insulin signalling proteins, miR-15b was associated with the insulin receptor in non-diabetic twins and knockdown of miR-15b/miR-16 in myocytes changed the levels of insulin signalling proteins. LBW in twins and undernutrition during pregnancy in rats were, in contrast to overt type 2 diabetes, associated with increased expression of miR-15b and/or miR-16. Elevated glucose and insulin suppressed miR-16 expression in vitro.

U2 - 10.1007/s00125-014-3434-2

DO - 10.1007/s00125-014-3434-2

M3 - Journal article

C2 - 25403480

SN - 0012-186X

VL - 58

SP - 363

EP - 373

JO - Diabetologia

JF - Diabetologia

IS - 2

ER -

Glucose tolerance is associated with differential expression of microRNAs in skeletal muscle: results from studies of twins with and without type 2 diabetes

Abstract

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