Glucose metabolism and metabolic flexibility in cultured skeletal muscle cells is related to exercise status in young male subjects

TY - JOUR

T1 - Glucose metabolism and metabolic flexibility in cultured skeletal muscle cells is related to exercise status in young male subjects

AU - Lund, Jenny

AU - S Tangen, Daniel

AU - Wiig, Håvard

AU - Stadheim, Hans K

AU - Helle, Siw A

AU - B Birk, Jesper

AU - Ingemann-Hansen, Thorsten

AU - Rustan, Arild C

AU - Thoresen, G Hege

AU - Wojtaszewski, Jørgen

AU - T Kase, Eili

AU - Jensen, Jørgen

N1 - CURIS 2018 NEXS 143

PY - 2018/3/15

Y1 - 2018/3/15

N2 - We hypothesised that skeletal muscles of healthy young people have a large variation in oxidative capacity and fibre-type composition, and aimed therefore to investigate glucose metabolism in biopsies and myotubes isolated from musculus vastus lateralis from healthy males with varying degrees of maximal oxygen uptake. Trained and intermediary trained subjects showed higher carbohydrate oxidation in vivo. Fibre-type distribution in biopsies and myotubes did not differ between groups. There was no correlation between fibre-type I expression in biopsies and myotubes. Myotubes from trained had higher deoxyglucose accumulation and fractional glucose oxidation (glucose oxidation relative to glucose uptake), and were also more sensitive to the suppressive action of acutely added oleic acid to the cells. Despite lack of correlation of fibre types between skeletal muscle biopsies and cultured cells, myotubes from trained subjects retained some of their phenotypes in vitro with respect to enhanced glucose metabolism and metabolic flexibility.

AB - We hypothesised that skeletal muscles of healthy young people have a large variation in oxidative capacity and fibre-type composition, and aimed therefore to investigate glucose metabolism in biopsies and myotubes isolated from musculus vastus lateralis from healthy males with varying degrees of maximal oxygen uptake. Trained and intermediary trained subjects showed higher carbohydrate oxidation in vivo. Fibre-type distribution in biopsies and myotubes did not differ between groups. There was no correlation between fibre-type I expression in biopsies and myotubes. Myotubes from trained had higher deoxyglucose accumulation and fractional glucose oxidation (glucose oxidation relative to glucose uptake), and were also more sensitive to the suppressive action of acutely added oleic acid to the cells. Despite lack of correlation of fibre types between skeletal muscle biopsies and cultured cells, myotubes from trained subjects retained some of their phenotypes in vitro with respect to enhanced glucose metabolism and metabolic flexibility.

KW - Training condition

KW - Myotubes

KW - Glucose

KW - Suppression

KW - Oxidation

U2 - 10.1080/13813455.2017.1369547

DO - 10.1080/13813455.2017.1369547

M3 - Journal article

C2 - 28862046

SN - 1381-3455

VL - 124

SP - 119

EP - 130

JO - Archives of Physiology and Biochemistry

JF - Archives of Physiology and Biochemistry

IS - 2

ER -