Global mapping of the yeast genetic interaction network

Amy Hin Yan Tong; Guillaume Lesage; Gary D Bader; Huiming Ding; Hong Xu; Xiaofeng Xin; James Young; Gabriel F Berriz; Renee L Brost; Michael Chang; YiQun Chen; Xin Cheng; Gordon Chua; Helena Friesen; Debra S Goldberg; Jennifer Haynes; Christine Humphries; Grace He; Shamiza Hussein; Lizhu Ke; Nevan Krogan; Zhijian Li; Joshua N Levinson; Hong Lu; Patrice Ménard; Christella Munyana; Ainslie B Parsons; Owen Ryan; Raffi Tonikian; Tania Roberts; Anne-Marie Sdicu; Jesse Shapiro; Bilal Sheikh; Bernhard Suter; Sharyl L Wong; Lan V Zhang; Hongwei Zhu; Christopher G Burd; Sean Munro; Chris Sander; Jasper Rine; Jack Greenblatt; Matthias Peter; Anthony Bretscher; Graham Bell; Frederick P Roth; Grant W Brown; Brenda Andrews; Howard Bussey; Charles Boone

doi:10.1126/science.1091317

Global mapping of the yeast genetic interaction network

Amy Hin Yan Tong, Guillaume Lesage, Gary D Bader, Huiming Ding, Hong Xu, Xiaofeng Xin, James Young, Gabriel F Berriz, Renee L Brost, Michael Chang, YiQun Chen, Xin Cheng, Gordon Chua, Helena Friesen, Debra S Goldberg, Jennifer Haynes, Christine Humphries, Grace He, Shamiza Hussein, Lizhu KeNevan Krogan, Zhijian Li, Joshua N Levinson, Hong Lu, Patrice Ménard, Christella Munyana, Ainslie B Parsons, Owen Ryan, Raffi Tonikian, Tania Roberts, Anne-Marie Sdicu, Jesse Shapiro, Bilal Sheikh, Bernhard Suter, Sharyl L Wong, Lan V Zhang, Hongwei Zhu, Christopher G Burd, Sean Munro, Chris Sander, Jasper Rine, Jack Greenblatt, Matthias Peter, Anthony Bretscher, Graham Bell, Frederick P Roth, Grant W Brown, Brenda Andrews, Howard Bussey, Charles Boone

1532 Citations (Scopus)

Abstract

A genetic interaction network containing approximately 1000 genes and approximately 4000 interactions was mapped by crossing mutations in 132 different query genes into a set of approximately 4700 viable gene yeast deletion mutants and scoring the double mutant progeny for fitness defects. Network connectivity was predictive of function because interactions often occurred among functionally related genes, and similar patterns of interactions tended to identify components of the same pathway. The genetic network exhibited dense local neighborhoods; therefore, the position of a gene on a partially mapped network is predictive of other genetic interactions. Because digenic interactions are common in yeast, similar networks may underlie the complex genetics associated with inherited phenotypes in other organisms.

Original language	English
Journal	Science (New York, N.Y.)
Volume	303
Issue number	5659
Pages (from-to)	808-13
Number of pages	5
DOIs	https://doi.org/10.1126/science.1091317
Publication status	Published - 2004
Externally published	Yes

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Tong, A. H. Y., Lesage, G., Bader, G. D., Ding, H., Xu, H., Xin, X., Young, J., Berriz, G. F., Brost, R. L., Chang, M., Chen, Y., Cheng, X., Chua, G., Friesen, H., Goldberg, D. S., Haynes, J., Humphries, C., He, G., Hussein, S., ... Boone, C. (2004). Global mapping of the yeast genetic interaction network. Science (New York, N.Y.), 303(5659), 808-13. https://doi.org/10.1126/science.1091317

Tong, AHY, Lesage, G, Bader, GD, Ding, H, Xu, H, Xin, X, Young, J, Berriz, GF, Brost, RL, Chang, M, Chen, Y, Cheng, X, Chua, G, Friesen, H, Goldberg, DS, Haynes, J, Humphries, C, He, G, Hussein, S, Ke, L, Krogan, N, Li, Z, Levinson, JN, Lu, H, Ménard, P, Munyana, C, Parsons, AB, Ryan, O, Tonikian, R, Roberts, T, Sdicu, A-M, Shapiro, J, Sheikh, B, Suter, B, Wong, SL, Zhang, LV, Zhu, H, Burd, CG, Munro, S, Sander, C, Rine, J, Greenblatt, J, Peter, M, Bretscher, A, Bell, G, Roth, FP, Brown, GW, Andrews, B, Bussey, H & Boone, C 2004, 'Global mapping of the yeast genetic interaction network', Science (New York, N.Y.), vol. 303, no. 5659, pp. 808-13. https://doi.org/10.1126/science.1091317

@article{3777cf705da511df928f000ea68e967b,

title = "Global mapping of the yeast genetic interaction network",

abstract = "A genetic interaction network containing approximately 1000 genes and approximately 4000 interactions was mapped by crossing mutations in 132 different query genes into a set of approximately 4700 viable gene yeast deletion mutants and scoring the double mutant progeny for fitness defects. Network connectivity was predictive of function because interactions often occurred among functionally related genes, and similar patterns of interactions tended to identify components of the same pathway. The genetic network exhibited dense local neighborhoods; therefore, the position of a gene on a partially mapped network is predictive of other genetic interactions. Because digenic interactions are common in yeast, similar networks may underlie the complex genetics associated with inherited phenotypes in other organisms.",

author = "Tong, {Amy Hin Yan} and Guillaume Lesage and Bader, {Gary D} and Huiming Ding and Hong Xu and Xiaofeng Xin and James Young and Berriz, {Gabriel F} and Brost, {Renee L} and Michael Chang and YiQun Chen and Xin Cheng and Gordon Chua and Helena Friesen and Goldberg, {Debra S} and Jennifer Haynes and Christine Humphries and Grace He and Shamiza Hussein and Lizhu Ke and Nevan Krogan and Zhijian Li and Levinson, {Joshua N} and Hong Lu and Patrice M{\'e}nard and Christella Munyana and Parsons, {Ainslie B} and Owen Ryan and Raffi Tonikian and Tania Roberts and Anne-Marie Sdicu and Jesse Shapiro and Bilal Sheikh and Bernhard Suter and Wong, {Sharyl L} and Zhang, {Lan V} and Hongwei Zhu and Burd, {Christopher G} and Sean Munro and Chris Sander and Jasper Rine and Jack Greenblatt and Matthias Peter and Anthony Bretscher and Graham Bell and Roth, {Frederick P} and Brown, {Grant W} and Brenda Andrews and Howard Bussey and Charles Boone",

note = "Keywords: Amino Acid Sequence; Computational Biology; Cystic Fibrosis; Gene Deletion; Genes, Essential; Genes, Fungal; Genetic Diseases, Inborn; Genotype; Humans; Molecular Sequence Data; Multifactorial Inheritance; Mutation; Phenotype; Polymorphism, Genetic; Retinitis Pigmentosa; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins",

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doi = "10.1126/science.1091317",

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T1 - Global mapping of the yeast genetic interaction network

AU - Tong, Amy Hin Yan

AU - Lesage, Guillaume

AU - Bader, Gary D

AU - Ding, Huiming

AU - Xu, Hong

AU - Xin, Xiaofeng

AU - Young, James

AU - Berriz, Gabriel F

AU - Brost, Renee L

AU - Chang, Michael

AU - Chen, YiQun

AU - Cheng, Xin

AU - Chua, Gordon

AU - Friesen, Helena

AU - Goldberg, Debra S

AU - Haynes, Jennifer

AU - Humphries, Christine

AU - He, Grace

AU - Hussein, Shamiza

AU - Ke, Lizhu

AU - Krogan, Nevan

AU - Li, Zhijian

AU - Levinson, Joshua N

AU - Lu, Hong

AU - Ménard, Patrice

AU - Munyana, Christella

AU - Parsons, Ainslie B

AU - Ryan, Owen

AU - Tonikian, Raffi

AU - Roberts, Tania

AU - Sdicu, Anne-Marie

AU - Shapiro, Jesse

AU - Sheikh, Bilal

AU - Suter, Bernhard

AU - Wong, Sharyl L

AU - Zhang, Lan V

AU - Zhu, Hongwei

AU - Burd, Christopher G

AU - Munro, Sean

AU - Sander, Chris

AU - Rine, Jasper

AU - Greenblatt, Jack

AU - Peter, Matthias

AU - Bretscher, Anthony

AU - Bell, Graham

AU - Roth, Frederick P

AU - Brown, Grant W

AU - Andrews, Brenda

AU - Bussey, Howard

AU - Boone, Charles

N1 - Keywords: Amino Acid Sequence; Computational Biology; Cystic Fibrosis; Gene Deletion; Genes, Essential; Genes, Fungal; Genetic Diseases, Inborn; Genotype; Humans; Molecular Sequence Data; Multifactorial Inheritance; Mutation; Phenotype; Polymorphism, Genetic; Retinitis Pigmentosa; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins

PY - 2004

Y1 - 2004

N2 - A genetic interaction network containing approximately 1000 genes and approximately 4000 interactions was mapped by crossing mutations in 132 different query genes into a set of approximately 4700 viable gene yeast deletion mutants and scoring the double mutant progeny for fitness defects. Network connectivity was predictive of function because interactions often occurred among functionally related genes, and similar patterns of interactions tended to identify components of the same pathway. The genetic network exhibited dense local neighborhoods; therefore, the position of a gene on a partially mapped network is predictive of other genetic interactions. Because digenic interactions are common in yeast, similar networks may underlie the complex genetics associated with inherited phenotypes in other organisms.

AB - A genetic interaction network containing approximately 1000 genes and approximately 4000 interactions was mapped by crossing mutations in 132 different query genes into a set of approximately 4700 viable gene yeast deletion mutants and scoring the double mutant progeny for fitness defects. Network connectivity was predictive of function because interactions often occurred among functionally related genes, and similar patterns of interactions tended to identify components of the same pathway. The genetic network exhibited dense local neighborhoods; therefore, the position of a gene on a partially mapped network is predictive of other genetic interactions. Because digenic interactions are common in yeast, similar networks may underlie the complex genetics associated with inherited phenotypes in other organisms.

U2 - 10.1126/science.1091317

DO - 10.1126/science.1091317

M3 - Journal article

C2 - 14764870

SN - 1095-9203

VL - 303

SP - 808

EP - 813

JO - Science (New York, N.Y.)

JF - Science (New York, N.Y.)

IS - 5659

ER -

Global mapping of the yeast genetic interaction network

Abstract

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