Global and 3D Spatial Assessment of Neuroinflammation in Rodent Models of Multiple Sclerosis

Shashank Gupta; Regine Egeholm Utoft; Henrik Hasseldam; Anja Schmidt-Christensen; Tine Dahlbæk Hannibal; Lisbeth Hansen; Nina Isabell Fransén Pettersson; Noopur Agarwal Gupta; Björn Rozell; Åsa Andersson; Dan Ingemar Holmberg

doi:10.1371/journal.pone.0076330

Global and 3D Spatial Assessment of Neuroinflammation in Rodent Models of Multiple Sclerosis

Shashank Gupta, Regine Egeholm Utoft, Henrik Hasseldam, Anja Schmidt-Christensen, Tine Dahlbæk Hannibal, Lisbeth Hansen, Nina Isabell Fransén Pettersson, Noopur Agarwal Gupta, Björn Rozell, Åsa Andersson, Dan Ingemar Holmberg

6 Citations (Scopus)

Abstract

Multiple Sclerosis (MS) is a progressive autoimmune inflammatory and demyelinating disease of the central nervous system (CNS). T cells play a key role in the progression of neuroinflammation in MS and also in the experimental autoimmune encephalomyelitis (EAE) animal models for the disease. A technology for quantitative and 3 dimensional (3D) spatial assessment of inflammation in this and other CNS inflammatory conditions is much needed. Here we present a procedure for 3D spatial assessment and global quantification of the development of neuroinflammation based on Optical Projection Tomography (OPT). Applying this approach to the analysis of rodent models of MS, we provide global quantitative data of the major inflammatory component as a function of the clinical course. Our data demonstrates a strong correlation between the development and progression of neuroinflammation and clinical disease in several mouse and a rat model of MS refining the information regarding the spatial dynamics of the inflammatory component in EAE. This method provides a powerful tool to investigate the effect of environmental and genetic forces and for assessing the therapeutic effects of drug therapy in animal models of MS and other neuroinflammatory/neurodegenerative disorders.

Original language	English
Article number	e76330
Journal	PLOS ONE
Volume	8
Issue number	10
Pages (from-to)	e76330
Number of pages	8
DOIs	https://doi.org/10.1371/journal.pone.0076330
Publication status	Published - 4 Oct 2013

Access to Document

10.1371/journal.pone.0076330

Cite this

@article{126142c95c354fce9b8193463daefae8,

title = "Global and 3D Spatial Assessment of Neuroinflammation in Rodent Models of Multiple Sclerosis",

abstract = "Multiple Sclerosis (MS) is a progressive autoimmune inflammatory and demyelinating disease of the central nervous system (CNS). T cells play a key role in the progression of neuroinflammation in MS and also in the experimental autoimmune encephalomyelitis (EAE) animal models for the disease. A technology for quantitative and 3 dimensional (3D) spatial assessment of inflammation in this and other CNS inflammatory conditions is much needed. Here we present a procedure for 3D spatial assessment and global quantification of the development of neuroinflammation based on Optical Projection Tomography (OPT). Applying this approach to the analysis of rodent models of MS, we provide global quantitative data of the major inflammatory component as a function of the clinical course. Our data demonstrates a strong correlation between the development and progression of neuroinflammation and clinical disease in several mouse and a rat model of MS refining the information regarding the spatial dynamics of the inflammatory component in EAE. This method provides a powerful tool to investigate the effect of environmental and genetic forces and for assessing the therapeutic effects of drug therapy in animal models of MS and other neuroinflammatory/neurodegenerative disorders.",

author = "Shashank Gupta and Utoft, {Regine Egeholm} and Henrik Hasseldam and Anja Schmidt-Christensen and Hannibal, {Tine Dahlb{\ae}k} and Lisbeth Hansen and {Frans{\'e}n Pettersson}, {Nina Isabell} and Gupta, {Noopur Agarwal} and Bj{\"o}rn Rozell and {\AA}sa Andersson and Holmberg, {Dan Ingemar}",

year = "2013",

month = oct,

day = "4",

doi = "10.1371/journal.pone.0076330",

language = "English",

volume = "8",

pages = "e76330",

journal = "PLoS Computational Biology",

issn = "1932-6203",

publisher = "Public Library of Science",

number = "10",

}

TY - JOUR

T1 - Global and 3D Spatial Assessment of Neuroinflammation in Rodent Models of Multiple Sclerosis

AU - Gupta, Shashank

AU - Utoft, Regine Egeholm

AU - Hasseldam, Henrik

AU - Schmidt-Christensen, Anja

AU - Hannibal, Tine Dahlbæk

AU - Hansen, Lisbeth

AU - Fransén Pettersson, Nina Isabell

AU - Gupta, Noopur Agarwal

AU - Rozell, Björn

AU - Andersson, Åsa

AU - Holmberg, Dan Ingemar

PY - 2013/10/4

Y1 - 2013/10/4

N2 - Multiple Sclerosis (MS) is a progressive autoimmune inflammatory and demyelinating disease of the central nervous system (CNS). T cells play a key role in the progression of neuroinflammation in MS and also in the experimental autoimmune encephalomyelitis (EAE) animal models for the disease. A technology for quantitative and 3 dimensional (3D) spatial assessment of inflammation in this and other CNS inflammatory conditions is much needed. Here we present a procedure for 3D spatial assessment and global quantification of the development of neuroinflammation based on Optical Projection Tomography (OPT). Applying this approach to the analysis of rodent models of MS, we provide global quantitative data of the major inflammatory component as a function of the clinical course. Our data demonstrates a strong correlation between the development and progression of neuroinflammation and clinical disease in several mouse and a rat model of MS refining the information regarding the spatial dynamics of the inflammatory component in EAE. This method provides a powerful tool to investigate the effect of environmental and genetic forces and for assessing the therapeutic effects of drug therapy in animal models of MS and other neuroinflammatory/neurodegenerative disorders.

AB - Multiple Sclerosis (MS) is a progressive autoimmune inflammatory and demyelinating disease of the central nervous system (CNS). T cells play a key role in the progression of neuroinflammation in MS and also in the experimental autoimmune encephalomyelitis (EAE) animal models for the disease. A technology for quantitative and 3 dimensional (3D) spatial assessment of inflammation in this and other CNS inflammatory conditions is much needed. Here we present a procedure for 3D spatial assessment and global quantification of the development of neuroinflammation based on Optical Projection Tomography (OPT). Applying this approach to the analysis of rodent models of MS, we provide global quantitative data of the major inflammatory component as a function of the clinical course. Our data demonstrates a strong correlation between the development and progression of neuroinflammation and clinical disease in several mouse and a rat model of MS refining the information regarding the spatial dynamics of the inflammatory component in EAE. This method provides a powerful tool to investigate the effect of environmental and genetic forces and for assessing the therapeutic effects of drug therapy in animal models of MS and other neuroinflammatory/neurodegenerative disorders.

U2 - 10.1371/journal.pone.0076330

DO - 10.1371/journal.pone.0076330

M3 - Journal article

SN - 1932-6203

VL - 8

SP - e76330

JO - PLoS Computational Biology

JF - PLoS Computational Biology

IS - 10

M1 - e76330

ER -

Global and 3D Spatial Assessment of Neuroinflammation in Rodent Models of Multiple Sclerosis

Abstract

Access to Document

Fingerprint

Cite this