Germline polymorphisms in an enhancer of PSIP1 are associated with progression-free survival in epithelial ovarian cancer

Juliet D French; Sharon E Johnatty; Yi Lu; Jonathan Beesley; Bo Gao; Murugan Kalimutho; Michelle J Henderson; Amanda J Russell; Siddhartha Kar; Xiaoqing Chen; Kristine M Hillman; Susanne Kaufmann; Haran Sivakumaran; Martin O'Reilly; Chen Wang; Darren J Korbie; Diether Lambrechts; Evelyn Despierre; Els Van Nieuwenhuysen; Sandrina Lambrechts; Ignace Vergote; Beth Karlan; Jenny Lester; Sandra Orsulic; Christine Walsh; Peter A Fasching; Matthias W Beckmann; Arif B Ekici; Alexander Hein; Keitaro Matsuo; Satoyo Hosono; Jacobus Pisterer; Peter Hillemanns; Toru Nakanishi; Yasushi Yatabe; Marc T Goodman; Galina Lurie; Rayna K Matsuno; Pamela J Thompson; Tanja Pejovic; Yukie Bean; Florian Heitz; Philipp Harter; Andreas Du Bois; Ira Schwaab; Estrid Hogdall; Susanne K Kjaer; Allan Jensen; Claus Hogdall; Svend Aage Engelholm; Australian Ovarian Cancer Study Group

doi:10.18632/oncotarget.7047

Germline polymorphisms in an enhancer of PSIP1 are associated with progression-free survival in epithelial ovarian cancer

Juliet D French, Sharon E Johnatty, Yi Lu, Jonathan Beesley, Bo Gao, Murugan Kalimutho, Michelle J Henderson, Amanda J Russell, Siddhartha Kar, Xiaoqing Chen, Kristine M Hillman, Susanne Kaufmann, Haran Sivakumaran, Martin O'Reilly, Chen Wang, Darren J Korbie, Diether Lambrechts, Evelyn Despierre, Els Van Nieuwenhuysen, Sandrina LambrechtsIgnace Vergote, Beth Karlan, Jenny Lester, Sandra Orsulic, Christine Walsh, Peter A Fasching, Matthias W Beckmann, Arif B Ekici, Alexander Hein, Keitaro Matsuo, Satoyo Hosono, Jacobus Pisterer, Peter Hillemanns, Toru Nakanishi, Yasushi Yatabe, Marc T Goodman, Galina Lurie, Rayna K Matsuno, Pamela J Thompson, Tanja Pejovic, Yukie Bean, Florian Heitz, Philipp Harter, Andreas Du Bois, Ira Schwaab, Estrid Hogdall, Susanne K Kjaer, Allan Jensen, Claus Hogdall, Svend Aage Engelholm, Australian Ovarian Cancer Study Group

Department of Clinical Medicine

12 Citations (Scopus)

Abstract

Women with epithelial ovarian cancer (EOC) are usually treated with platinum/taxane therapy after cytoreductive surgery but there is considerable inter-individual variation in response. To identify germline single-nucleotide polymorphisms (SNPs) that contribute to variations in individual responses to chemotherapy, we carried out a multi-phase genome-wide association study (GWAS) in 1,244 women diagnosed with serous EOC who were treated with the same first-line chemotherapy, carboplatin and paclitaxel. We identified two SNPs (rs7874043 and rs72700653) in TTC39B (best P=7×10^-5, HR=1.90, for rs7874043) associated with progression-free survival (PFS). Functional analyses show that both SNPs lie in a putative regulatory element (PRE) that physically interacts with the promoters of PSIP1, CCDC171 and an alternative promoter of TTC39B. The C allele of rs7874043 is associated with poor PFS and showed increased binding of the Sp1 transcription factor, which is critical for chromatin interactions with PSIP1. Silencing of PSIP1 significantly impaired DNA damage-induced Rad51 nuclear foci and reduced cell viability in ovarian cancer lines. PSIP1 (PC4 and SFRS1 Interacting Protein 1) is known to protect cells from stress-induced apoptosis, and high expression is associated with poor PFS in EOC patients. We therefore suggest that the minor allele of rs7874043 confers poor PFS by increasing PSIP1 expression.

Original language	English
Journal	OncoTarget
Volume	7
Issue number	6
Pages (from-to)	6353-68
Number of pages	16
ISSN	1949-2553
DOIs	https://doi.org/10.18632/oncotarget.7047
Publication status	Published - 2016

Keywords

Adaptor Proteins, Signal Transducing
Apoptosis
Biomarkers, Tumor
Cell Proliferation
Chromatin Immunoprecipitation
Cohort Studies
Cystadenocarcinoma, Serous
Electrophoretic Mobility Shift Assay
Enhancer Elements, Genetic
Fallopian Tube Neoplasms
Female
Follow-Up Studies
Germ-Line Mutation
Humans
Ovarian Neoplasms
Peritoneal Neoplasms
Polymorphism, Single Nucleotide
Prognosis
RNA, Messenger
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Survival Rate
Transcription Factors
Tumor Cells, Cultured
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.18632/oncotarget.7047

Cite this

French, J. D., Johnatty, S. E., Lu, Y., Beesley, J., Gao, B., Kalimutho, M., Henderson, M. J., Russell, A. J., Kar, S., Chen, X., Hillman, K. M., Kaufmann, S., Sivakumaran, H., O'Reilly, M., Wang, C., Korbie, D. J., Lambrechts, D., Despierre, E., Van Nieuwenhuysen, E., ... Australian Ovarian Cancer Study Group (2016). Germline polymorphisms in an enhancer of PSIP1 are associated with progression-free survival in epithelial ovarian cancer. OncoTarget, 7(6), 6353-68. https://doi.org/10.18632/oncotarget.7047

French, JD, Johnatty, SE, Lu, Y, Beesley, J, Gao, B, Kalimutho, M, Henderson, MJ, Russell, AJ, Kar, S, Chen, X, Hillman, KM, Kaufmann, S, Sivakumaran, H, O'Reilly, M, Wang, C, Korbie, DJ, Lambrechts, D, Despierre, E, Van Nieuwenhuysen, E, Lambrechts, S, Vergote, I, Karlan, B, Lester, J, Orsulic, S, Walsh, C, Fasching, PA, Beckmann, MW, Ekici, AB, Hein, A, Matsuo, K, Hosono, S, Pisterer, J, Hillemanns, P, Nakanishi, T, Yatabe, Y, Goodman, MT, Lurie, G, Matsuno, RK, Thompson, PJ, Pejovic, T, Bean, Y, Heitz, F, Harter, P, Du Bois, A, Schwaab, I, Hogdall, E , Kjaer, SK, Jensen, A, Hogdall, C, Engelholm, SA & Australian Ovarian Cancer Study Group 2016, 'Germline polymorphisms in an enhancer of PSIP1 are associated with progression-free survival in epithelial ovarian cancer', OncoTarget, vol. 7, no. 6, pp. 6353-68. https://doi.org/10.18632/oncotarget.7047

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title = "Germline polymorphisms in an enhancer of PSIP1 are associated with progression-free survival in epithelial ovarian cancer",

abstract = "Women with epithelial ovarian cancer (EOC) are usually treated with platinum/taxane therapy after cytoreductive surgery but there is considerable inter-individual variation in response. To identify germline single-nucleotide polymorphisms (SNPs) that contribute to variations in individual responses to chemotherapy, we carried out a multi-phase genome-wide association study (GWAS) in 1,244 women diagnosed with serous EOC who were treated with the same first-line chemotherapy, carboplatin and paclitaxel. We identified two SNPs (rs7874043 and rs72700653) in TTC39B (best P=7×10-5, HR=1.90, for rs7874043) associated with progression-free survival (PFS). Functional analyses show that both SNPs lie in a putative regulatory element (PRE) that physically interacts with the promoters of PSIP1, CCDC171 and an alternative promoter of TTC39B. The C allele of rs7874043 is associated with poor PFS and showed increased binding of the Sp1 transcription factor, which is critical for chromatin interactions with PSIP1. Silencing of PSIP1 significantly impaired DNA damage-induced Rad51 nuclear foci and reduced cell viability in ovarian cancer lines. PSIP1 (PC4 and SFRS1 Interacting Protein 1) is known to protect cells from stress-induced apoptosis, and high expression is associated with poor PFS in EOC patients. We therefore suggest that the minor allele of rs7874043 confers poor PFS by increasing PSIP1 expression.",

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author = "French, {Juliet D} and Johnatty, {Sharon E} and Yi Lu and Jonathan Beesley and Bo Gao and Murugan Kalimutho and Henderson, {Michelle J} and Russell, {Amanda J} and Siddhartha Kar and Xiaoqing Chen and Hillman, {Kristine M} and Susanne Kaufmann and Haran Sivakumaran and Martin O'Reilly and Chen Wang and Korbie, {Darren J} and Diether Lambrechts and Evelyn Despierre and {Van Nieuwenhuysen}, Els and Sandrina Lambrechts and Ignace Vergote and Beth Karlan and Jenny Lester and Sandra Orsulic and Christine Walsh and Fasching, {Peter A} and Beckmann, {Matthias W} and Ekici, {Arif B} and Alexander Hein and Keitaro Matsuo and Satoyo Hosono and Jacobus Pisterer and Peter Hillemanns and Toru Nakanishi and Yasushi Yatabe and Goodman, {Marc T} and Galina Lurie and Matsuno, {Rayna K} and Thompson, {Pamela J} and Tanja Pejovic and Yukie Bean and Florian Heitz and Philipp Harter and {Du Bois}, Andreas and Ira Schwaab and Estrid Hogdall and Kjaer, {Susanne K} and Allan Jensen and Claus Hogdall and Engelholm, {Svend Aage} and {Australian Ovarian Cancer Study Group}",

year = "2016",

doi = "10.18632/oncotarget.7047",

language = "English",

volume = "7",

pages = "6353--68",

journal = "Oncotarget",

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T1 - Germline polymorphisms in an enhancer of PSIP1 are associated with progression-free survival in epithelial ovarian cancer

AU - French, Juliet D

AU - Johnatty, Sharon E

AU - Lu, Yi

AU - Beesley, Jonathan

AU - Gao, Bo

AU - Kalimutho, Murugan

AU - Henderson, Michelle J

AU - Russell, Amanda J

AU - Kar, Siddhartha

AU - Chen, Xiaoqing

AU - Hillman, Kristine M

AU - Kaufmann, Susanne

AU - Sivakumaran, Haran

AU - O'Reilly, Martin

AU - Wang, Chen

AU - Korbie, Darren J

AU - Lambrechts, Diether

AU - Despierre, Evelyn

AU - Van Nieuwenhuysen, Els

AU - Lambrechts, Sandrina

AU - Vergote, Ignace

AU - Karlan, Beth

AU - Lester, Jenny

AU - Orsulic, Sandra

AU - Walsh, Christine

AU - Fasching, Peter A

AU - Beckmann, Matthias W

AU - Ekici, Arif B

AU - Hein, Alexander

AU - Matsuo, Keitaro

AU - Hosono, Satoyo

AU - Pisterer, Jacobus

AU - Hillemanns, Peter

AU - Nakanishi, Toru

AU - Yatabe, Yasushi

AU - Goodman, Marc T

AU - Lurie, Galina

AU - Matsuno, Rayna K

AU - Thompson, Pamela J

AU - Pejovic, Tanja

AU - Bean, Yukie

AU - Heitz, Florian

AU - Harter, Philipp

AU - Du Bois, Andreas

AU - Schwaab, Ira

AU - Hogdall, Estrid

AU - Kjaer, Susanne K

AU - Jensen, Allan

AU - Hogdall, Claus

AU - Engelholm, Svend Aage

AU - Australian Ovarian Cancer Study Group

PY - 2016

Y1 - 2016

N2 - Women with epithelial ovarian cancer (EOC) are usually treated with platinum/taxane therapy after cytoreductive surgery but there is considerable inter-individual variation in response. To identify germline single-nucleotide polymorphisms (SNPs) that contribute to variations in individual responses to chemotherapy, we carried out a multi-phase genome-wide association study (GWAS) in 1,244 women diagnosed with serous EOC who were treated with the same first-line chemotherapy, carboplatin and paclitaxel. We identified two SNPs (rs7874043 and rs72700653) in TTC39B (best P=7×10-5, HR=1.90, for rs7874043) associated with progression-free survival (PFS). Functional analyses show that both SNPs lie in a putative regulatory element (PRE) that physically interacts with the promoters of PSIP1, CCDC171 and an alternative promoter of TTC39B. The C allele of rs7874043 is associated with poor PFS and showed increased binding of the Sp1 transcription factor, which is critical for chromatin interactions with PSIP1. Silencing of PSIP1 significantly impaired DNA damage-induced Rad51 nuclear foci and reduced cell viability in ovarian cancer lines. PSIP1 (PC4 and SFRS1 Interacting Protein 1) is known to protect cells from stress-induced apoptosis, and high expression is associated with poor PFS in EOC patients. We therefore suggest that the minor allele of rs7874043 confers poor PFS by increasing PSIP1 expression.

AB - Women with epithelial ovarian cancer (EOC) are usually treated with platinum/taxane therapy after cytoreductive surgery but there is considerable inter-individual variation in response. To identify germline single-nucleotide polymorphisms (SNPs) that contribute to variations in individual responses to chemotherapy, we carried out a multi-phase genome-wide association study (GWAS) in 1,244 women diagnosed with serous EOC who were treated with the same first-line chemotherapy, carboplatin and paclitaxel. We identified two SNPs (rs7874043 and rs72700653) in TTC39B (best P=7×10-5, HR=1.90, for rs7874043) associated with progression-free survival (PFS). Functional analyses show that both SNPs lie in a putative regulatory element (PRE) that physically interacts with the promoters of PSIP1, CCDC171 and an alternative promoter of TTC39B. The C allele of rs7874043 is associated with poor PFS and showed increased binding of the Sp1 transcription factor, which is critical for chromatin interactions with PSIP1. Silencing of PSIP1 significantly impaired DNA damage-induced Rad51 nuclear foci and reduced cell viability in ovarian cancer lines. PSIP1 (PC4 and SFRS1 Interacting Protein 1) is known to protect cells from stress-induced apoptosis, and high expression is associated with poor PFS in EOC patients. We therefore suggest that the minor allele of rs7874043 confers poor PFS by increasing PSIP1 expression.

KW - Adaptor Proteins, Signal Transducing

KW - Apoptosis

KW - Biomarkers, Tumor

KW - Cell Proliferation

KW - Chromatin Immunoprecipitation

KW - Cohort Studies

KW - Cystadenocarcinoma, Serous

KW - Electrophoretic Mobility Shift Assay

KW - Enhancer Elements, Genetic

KW - Fallopian Tube Neoplasms

KW - Female

KW - Follow-Up Studies

KW - Germ-Line Mutation

KW - Humans

KW - Ovarian Neoplasms

KW - Peritoneal Neoplasms

KW - Polymorphism, Single Nucleotide

KW - Prognosis

KW - RNA, Messenger

KW - Real-Time Polymerase Chain Reaction

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Survival Rate

KW - Transcription Factors

KW - Tumor Cells, Cultured

KW - Journal Article

KW - Research Support, N.I.H., Extramural

KW - Research Support, Non-U.S. Gov't

KW - Research Support, U.S. Gov't, Non-P.H.S.

U2 - 10.18632/oncotarget.7047

DO - 10.18632/oncotarget.7047

M3 - Journal article

C2 - 26840454

SN - 1949-2553

VL - 7

SP - 6353

EP - 6368

JO - Oncotarget

JF - Oncotarget

IS - 6

ER -

Germline polymorphisms in an enhancer of PSIP1 are associated with progression-free survival in epithelial ovarian cancer

Abstract

Keywords

UN SDGs

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