Germin‐like oxalate oxidase, a H2O2‐producing enzyme, accumulates in barley attacked by the powdery mildew fungus

Ziguo Zhang; David B. Collinge; Hans Thordal‐Christensen

doi:10.1046/j.1365-313X.1995.08010139.x

Germin‐like oxalate oxidase, a H₂O₂‐producing enzyme, accumulates in barley attacked by the powdery mildew fungus

Ziguo Zhang, David B. Collinge, Hans Thordal‐Christensen^*

^*Corresponding author for this work

173 Citations (Scopus)

Abstract

Germin‐like oxalate oxidase is an oligomeric enzyme which generates H₂O₂. This paper reports increased activity of this enzyme in association with the response of barley to the powdery mildew fungus, Erysiphe graminis f.sp. hordei. The increase is detected in a colorimetric assay as well as on activity blots using extracts of both resistant and susceptible leaves. The increase is generally apparent from 24 h after inoculation. From 48 h after inoculation, there is an approximately 10‐fold higher oxalate oxidase activity in the samples from inoculated plants compared with the controls. The oxalate oxidase activity increase appears 1–3 days earlier than PR‐1 protein accumulation. SDS—PAGE analysis suggests that this pathogen‐response oxalate oxidase is different from a commercially available barley oxalate oxidase, and from a constitutive barley root oxalate oxidase.

Original language	English
Journal	The Plant Journal
Volume	8
Issue number	1
Pages (from-to)	139-145
Number of pages	7
ISSN	0960-7412
DOIs	https://doi.org/10.1046/j.1365-313X.1995.08010139.x
Publication status	Published - 1 Jan 1995

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title = "Germin‐like oxalate oxidase, a H2O2‐producing enzyme, accumulates in barley attacked by the powdery mildew fungus",

abstract = "Germin‐like oxalate oxidase is an oligomeric enzyme which generates H2O2. This paper reports increased activity of this enzyme in association with the response of barley to the powdery mildew fungus, Erysiphe graminis f.sp. hordei. The increase is detected in a colorimetric assay as well as on activity blots using extracts of both resistant and susceptible leaves. The increase is generally apparent from 24 h after inoculation. From 48 h after inoculation, there is an approximately 10‐fold higher oxalate oxidase activity in the samples from inoculated plants compared with the controls. The oxalate oxidase activity increase appears 1–3 days earlier than PR‐1 protein accumulation. SDS—PAGE analysis suggests that this pathogen‐response oxalate oxidase is different from a commercially available barley oxalate oxidase, and from a constitutive barley root oxalate oxidase.",

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T1 - Germin‐like oxalate oxidase, a H2O2‐producing enzyme, accumulates in barley attacked by the powdery mildew fungus

AU - Zhang, Ziguo

AU - Collinge, David B.

AU - Thordal‐Christensen, Hans

PY - 1995/1/1

Y1 - 1995/1/1

N2 - Germin‐like oxalate oxidase is an oligomeric enzyme which generates H2O2. This paper reports increased activity of this enzyme in association with the response of barley to the powdery mildew fungus, Erysiphe graminis f.sp. hordei. The increase is detected in a colorimetric assay as well as on activity blots using extracts of both resistant and susceptible leaves. The increase is generally apparent from 24 h after inoculation. From 48 h after inoculation, there is an approximately 10‐fold higher oxalate oxidase activity in the samples from inoculated plants compared with the controls. The oxalate oxidase activity increase appears 1–3 days earlier than PR‐1 protein accumulation. SDS—PAGE analysis suggests that this pathogen‐response oxalate oxidase is different from a commercially available barley oxalate oxidase, and from a constitutive barley root oxalate oxidase.

AB - Germin‐like oxalate oxidase is an oligomeric enzyme which generates H2O2. This paper reports increased activity of this enzyme in association with the response of barley to the powdery mildew fungus, Erysiphe graminis f.sp. hordei. The increase is detected in a colorimetric assay as well as on activity blots using extracts of both resistant and susceptible leaves. The increase is generally apparent from 24 h after inoculation. From 48 h after inoculation, there is an approximately 10‐fold higher oxalate oxidase activity in the samples from inoculated plants compared with the controls. The oxalate oxidase activity increase appears 1–3 days earlier than PR‐1 protein accumulation. SDS—PAGE analysis suggests that this pathogen‐response oxalate oxidase is different from a commercially available barley oxalate oxidase, and from a constitutive barley root oxalate oxidase.

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DO - 10.1046/j.1365-313X.1995.08010139.x

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Germin‐like oxalate oxidase, a H₂O₂‐producing enzyme, accumulates in barley attacked by the powdery mildew fungus

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