TY - JOUR
T1 - Genomic and proteomic analyses of Prdm5 reveal interactions with insulator binding proteins in embryonic stem cells
AU - Galli, Giorgio Giacomo
AU - Carrara, Matteo
AU - Francavilla, Chiara
AU - Honnens de Lichtenberg, Kristian
AU - Olsen, Jesper Velgaard
AU - Calogero, Raffaele Adolfo
AU - Lund, Anders H.
PY - 2013/11/15
Y1 - 2013/11/15
N2 - PRDM proteins belong to the SET domain protein family, which is involved in the regulation of gene expression. Although few PRDM members possess histone methyltransferase activity, the molecular mechanisms by which the other members exert transcriptional regulation remain to be delineated. In this study, we find that Prdm5 is highly expressed in mouse embryonic stem (mES) cells and exploit this cellular system to characterize molecular functions of Prdm5. By combining proteomics and nextgeneration sequencing technologies, we identify Prdm5 interaction partners and genomic occupancy. We demonstrate that although Prdm5 is dispensable for mES cell maintenance, it directly targets genomic regions involved in early embryonic development and affects the expression of a subset of developmental regulators during cell differentiation. Importantly, Prdm5 interacts with Ctcf, cohesin, and TFIIIC and cooccupies genomic loci. In summary, our data indicate how Prdm5 modulates transcription by interacting with factors involved in genome organization in mouse embryonic stem cells.
AB - PRDM proteins belong to the SET domain protein family, which is involved in the regulation of gene expression. Although few PRDM members possess histone methyltransferase activity, the molecular mechanisms by which the other members exert transcriptional regulation remain to be delineated. In this study, we find that Prdm5 is highly expressed in mouse embryonic stem (mES) cells and exploit this cellular system to characterize molecular functions of Prdm5. By combining proteomics and nextgeneration sequencing technologies, we identify Prdm5 interaction partners and genomic occupancy. We demonstrate that although Prdm5 is dispensable for mES cell maintenance, it directly targets genomic regions involved in early embryonic development and affects the expression of a subset of developmental regulators during cell differentiation. Importantly, Prdm5 interacts with Ctcf, cohesin, and TFIIIC and cooccupies genomic loci. In summary, our data indicate how Prdm5 modulates transcription by interacting with factors involved in genome organization in mouse embryonic stem cells.
U2 - 10.1128/MCB.00545-13
DO - 10.1128/MCB.00545-13
M3 - Journal article
C2 - 24043305
SN - 0270-7306
JO - Molecular and Cellular Biology
JF - Molecular and Cellular Biology
ER -