Genomic and functional integrity of the hematopoietic system requires tolerance of oxidative DNA lesions

Ana Martín-Pardillos; Anastasia Tsaalbi-Shtylik; Si Chen; Seka Lazare; Ronald P. Van Os; Albertina Dethmers-Ausema; Nima Borhan Fakouri; Matthias Bosshard; Rossana Aprigliano; Barbara Van Loon; Daniela C.F. Salvatori; Keiji Hashimoto; Celia Dingemanse Van Der Spek; Masaaki Moriya; Lene Juel Rasmussen; Gerald De Haan; Marc H.G.P. Raaijmakers; Niels De Wind

doi:10.1182/blood-2017-01-764274

Genomic and functional integrity of the hematopoietic system requires tolerance of oxidative DNA lesions

Ana Martín-Pardillos, Anastasia Tsaalbi-Shtylik, Si Chen, Seka Lazare, Ronald P. Van Os, Albertina Dethmers-Ausema, Nima Borhan Fakouri, Matthias Bosshard, Rossana Aprigliano, Barbara Van Loon, Daniela C.F. Salvatori, Keiji Hashimoto, Celia Dingemanse Van Der Spek, Masaaki Moriya, Lene Juel Rasmussen, Gerald De Haan, Marc H.G.P. Raaijmakers^*, Niels De Wind

^*Corresponding author for this work

15 Citations (Scopus)

Abstract

Endogenous DNA damage is causally associated with the functional decline and transformation of stem cells that characterize aging. DNA lesions that have escaped DNA repair can induce replication stress and genomic breaks that induce senescence and apoptosis. It is not clear how stem and proliferating cells cope with accumulating endogenous DNA lesions and how these ultimately affect the physiology of cells and tissues. Here we have addressed these questions by investigating the hematopoietic system of mice deficient for Rev1, a core factor in DNA translesion synthesis (TLS), the postreplicative bypass of damaged nucleotides. Rev1 hematopoietic stem and progenitor cells displayed compromised proliferation, and replication stress that could be rescued with an antioxidant. The additional disruption of Xpc, essential for global-genome nucleotide excision repair (ggNER) of helix-distorting nucleotide lesions, resulted in the perinatal loss of hematopoietic stem cells, progressive loss of bone marrow, and fatal aplastic anemia between 3 and 4 months of age. This was associated with replication stress, genomic breaks, DNA damage signaling, senescence, and apoptosis in bone marrow. Surprisingly, the collapse of the Rev1Xpc bone marrow was associated with progressive mitochondrial dysfunction and consequent exacerbation of oxidative stress. These data reveal that, to protect its genomic and functional integrity, the hematopoietic system critically depends on the combined activities of repair and replication of helix-distorting oxidative nucleotide lesions by ggNER and Rev1-dependent TLS, respectively. The error-prone nature of TLS may provide mechanistic understanding of the accumulation of mutations in the hematopoietic system upon aging.

Original language	English
Journal	Blood
Volume	130
Issue number	13
Pages (from-to)	1523-1534
Number of pages	12
ISSN	0006-4971
DOIs	https://doi.org/10.1182/blood-2017-01-764274
Publication status	Published - 2017

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Martín-Pardillos, A., Tsaalbi-Shtylik, A., Chen, S., Lazare, S., Van Os, R. P., Dethmers-Ausema, A., Fakouri, N. B., Bosshard, M., Aprigliano, R., Van Loon, B., Salvatori, D. C. F., Hashimoto, K., Van Der Spek, C. D., Moriya, M., Rasmussen, L. J., De Haan, G., Raaijmakers, M. H. G. P., & De Wind, N. (2017). Genomic and functional integrity of the hematopoietic system requires tolerance of oxidative DNA lesions. Blood, 130(13), 1523-1534. https://doi.org/10.1182/blood-2017-01-764274

Martín-Pardillos, A, Tsaalbi-Shtylik, A, Chen, S, Lazare, S, Van Os, RP, Dethmers-Ausema, A, Fakouri, NB, Bosshard, M, Aprigliano, R, Van Loon, B, Salvatori, DCF, Hashimoto, K, Van Der Spek, CD, Moriya, M, Rasmussen, LJ, De Haan, G, Raaijmakers, MHGP & De Wind, N 2017, 'Genomic and functional integrity of the hematopoietic system requires tolerance of oxidative DNA lesions', Blood, vol. 130, no. 13, pp. 1523-1534. https://doi.org/10.1182/blood-2017-01-764274

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title = "Genomic and functional integrity of the hematopoietic system requires tolerance of oxidative DNA lesions",

abstract = "Endogenous DNA damage is causally associated with the functional decline and transformation of stem cells that characterize aging. DNA lesions that have escaped DNA repair can induce replication stress and genomic breaks that induce senescence and apoptosis. It is not clear how stem and proliferating cells cope with accumulating endogenous DNA lesions and how these ultimately affect the physiology of cells and tissues. Here we have addressed these questions by investigating the hematopoietic system of mice deficient for Rev1, a core factor in DNA translesion synthesis (TLS), the postreplicative bypass of damaged nucleotides. Rev1 hematopoietic stem and progenitor cells displayed compromised proliferation, and replication stress that could be rescued with an antioxidant. The additional disruption of Xpc, essential for global-genome nucleotide excision repair (ggNER) of helix-distorting nucleotide lesions, resulted in the perinatal loss of hematopoietic stem cells, progressive loss of bone marrow, and fatal aplastic anemia between 3 and 4 months of age. This was associated with replication stress, genomic breaks, DNA damage signaling, senescence, and apoptosis in bone marrow. Surprisingly, the collapse of the Rev1Xpc bone marrow was associated with progressive mitochondrial dysfunction and consequent exacerbation of oxidative stress. These data reveal that, to protect its genomic and functional integrity, the hematopoietic system critically depends on the combined activities of repair and replication of helix-distorting oxidative nucleotide lesions by ggNER and Rev1-dependent TLS, respectively. The error-prone nature of TLS may provide mechanistic understanding of the accumulation of mutations in the hematopoietic system upon aging.",

author = "Ana Mart{\'i}n-Pardillos and Anastasia Tsaalbi-Shtylik and Si Chen and Seka Lazare and {Van Os}, {Ronald P.} and Albertina Dethmers-Ausema and Fakouri, {Nima Borhan} and Matthias Bosshard and Rossana Aprigliano and {Van Loon}, Barbara and Salvatori, {Daniela C.F.} and Keiji Hashimoto and {Van Der Spek}, {Celia Dingemanse} and Masaaki Moriya and Rasmussen, {Lene Juel} and {De Haan}, Gerald and Raaijmakers, {Marc H.G.P.} and {De Wind}, Niels",

year = "2017",

doi = "10.1182/blood-2017-01-764274",

language = "English",

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T1 - Genomic and functional integrity of the hematopoietic system requires tolerance of oxidative DNA lesions

AU - Martín-Pardillos, Ana

AU - Tsaalbi-Shtylik, Anastasia

AU - Chen, Si

AU - Lazare, Seka

AU - Van Os, Ronald P.

AU - Dethmers-Ausema, Albertina

AU - Fakouri, Nima Borhan

AU - Bosshard, Matthias

AU - Aprigliano, Rossana

AU - Van Loon, Barbara

AU - Salvatori, Daniela C.F.

AU - Hashimoto, Keiji

AU - Van Der Spek, Celia Dingemanse

AU - Moriya, Masaaki

AU - Rasmussen, Lene Juel

AU - De Haan, Gerald

AU - Raaijmakers, Marc H.G.P.

AU - De Wind, Niels

PY - 2017

Y1 - 2017

N2 - Endogenous DNA damage is causally associated with the functional decline and transformation of stem cells that characterize aging. DNA lesions that have escaped DNA repair can induce replication stress and genomic breaks that induce senescence and apoptosis. It is not clear how stem and proliferating cells cope with accumulating endogenous DNA lesions and how these ultimately affect the physiology of cells and tissues. Here we have addressed these questions by investigating the hematopoietic system of mice deficient for Rev1, a core factor in DNA translesion synthesis (TLS), the postreplicative bypass of damaged nucleotides. Rev1 hematopoietic stem and progenitor cells displayed compromised proliferation, and replication stress that could be rescued with an antioxidant. The additional disruption of Xpc, essential for global-genome nucleotide excision repair (ggNER) of helix-distorting nucleotide lesions, resulted in the perinatal loss of hematopoietic stem cells, progressive loss of bone marrow, and fatal aplastic anemia between 3 and 4 months of age. This was associated with replication stress, genomic breaks, DNA damage signaling, senescence, and apoptosis in bone marrow. Surprisingly, the collapse of the Rev1Xpc bone marrow was associated with progressive mitochondrial dysfunction and consequent exacerbation of oxidative stress. These data reveal that, to protect its genomic and functional integrity, the hematopoietic system critically depends on the combined activities of repair and replication of helix-distorting oxidative nucleotide lesions by ggNER and Rev1-dependent TLS, respectively. The error-prone nature of TLS may provide mechanistic understanding of the accumulation of mutations in the hematopoietic system upon aging.

AB - Endogenous DNA damage is causally associated with the functional decline and transformation of stem cells that characterize aging. DNA lesions that have escaped DNA repair can induce replication stress and genomic breaks that induce senescence and apoptosis. It is not clear how stem and proliferating cells cope with accumulating endogenous DNA lesions and how these ultimately affect the physiology of cells and tissues. Here we have addressed these questions by investigating the hematopoietic system of mice deficient for Rev1, a core factor in DNA translesion synthesis (TLS), the postreplicative bypass of damaged nucleotides. Rev1 hematopoietic stem and progenitor cells displayed compromised proliferation, and replication stress that could be rescued with an antioxidant. The additional disruption of Xpc, essential for global-genome nucleotide excision repair (ggNER) of helix-distorting nucleotide lesions, resulted in the perinatal loss of hematopoietic stem cells, progressive loss of bone marrow, and fatal aplastic anemia between 3 and 4 months of age. This was associated with replication stress, genomic breaks, DNA damage signaling, senescence, and apoptosis in bone marrow. Surprisingly, the collapse of the Rev1Xpc bone marrow was associated with progressive mitochondrial dysfunction and consequent exacerbation of oxidative stress. These data reveal that, to protect its genomic and functional integrity, the hematopoietic system critically depends on the combined activities of repair and replication of helix-distorting oxidative nucleotide lesions by ggNER and Rev1-dependent TLS, respectively. The error-prone nature of TLS may provide mechanistic understanding of the accumulation of mutations in the hematopoietic system upon aging.

U2 - 10.1182/blood-2017-01-764274

DO - 10.1182/blood-2017-01-764274

M3 - Journal article

C2 - 28827409

AN - SCOPUS:85030031077

SN - 0006-4971

VL - 130

SP - 1523

EP - 1534

JO - Blood

JF - Blood

IS - 13

ER -

Genomic and functional integrity of the hematopoietic system requires tolerance of oxidative DNA lesions

Abstract

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