Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases

Lin Lin; Yong Liu; Fengping Xu; Jinrong Huang; Tina Fuglsang Daugaard; Trine Skov Petersen; Bettina Hansen; Lingfei Ye; Qing Zhou; Fang Fang; Ling Yang; Shengting Li; Lasse Fløe; Kristopher Torp Jensen; Ellen Shrock; Fang Chen; Huanming Yang; Jian Wang; Xin Liu; Xun Xu; Lars Bolund; Anders Lade Nielsen; Yonglun Luo

doi:10.1093/gigascience/giy011

Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases

Lin Lin, Yong Liu, Fengping Xu, Jinrong Huang, Tina Fuglsang Daugaard, Trine Skov Petersen, Bettina Hansen, Lingfei Ye, Qing Zhou, Fang Fang, Ling Yang, Shengting Li, Lasse Fløe, Kristopher Torp Jensen, Ellen Shrock, Fang Chen, Huanming Yang, Jian Wang, Xin Liu, Xun XuLars Bolund, Anders Lade Nielsen, Yonglun Luo

Genome Research and Molecular Bio Medicine

27 Citations (Scopus)

73 Downloads (Pure)

Original language	English
Journal	GigaScience
Volume	7
Issue number	3
Number of pages	19
ISSN	2047-217X
DOIs	https://doi.org/10.1093/gigascience/giy011
Publication status	Published - 1 Mar 2018

Keywords

DNA methylation
CRISPR
Cas9
DNMT3A
DNMT3B
dCas9
specificity
off-targets
epigenome editing

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10.1093/gigascience/giy011Licence: CC BY

Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferasesFinal published version, 4.54 MBLicence: CC BY

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Lin, L., Liu, Y., Xu, F., Huang, J., Daugaard, T. F., Petersen, T. S., Hansen, B., Ye, L., Zhou, Q., Fang, F., Yang, L., Li, S., Fløe, L., Jensen, K. T., Shrock, E., Chen, F., Yang, H., Wang, J., Liu, X., ... Luo, Y. (2018). Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases. GigaScience, 7(3). https://doi.org/10.1093/gigascience/giy011

Lin, L, Liu, Y, Xu, F, Huang, J, Daugaard, TF, Petersen, TS, Hansen, B, Ye, L, Zhou, Q, Fang, F, Yang, L, Li, S, Fløe, L, Jensen, KT, Shrock, E, Chen, F, Yang, H, Wang, J, Liu, X, Xu, X, Bolund, L, Nielsen, AL & Luo, Y 2018, 'Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases', GigaScience, vol. 7, no. 3. https://doi.org/10.1093/gigascience/giy011

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title = "Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases",

keywords = "DNA methylation, CRISPR, Cas9, DNMT3A, DNMT3B, dCas9, specificity, off-targets, epigenome editing",

author = "Lin Lin and Yong Liu and Fengping Xu and Jinrong Huang and Daugaard, {Tina Fuglsang} and Petersen, {Trine Skov} and Bettina Hansen and Lingfei Ye and Qing Zhou and Fang Fang and Ling Yang and Shengting Li and Lasse Fl{\o}e and Jensen, {Kristopher Torp} and Ellen Shrock and Fang Chen and Huanming Yang and Jian Wang and Xin Liu and Xun Xu and Lars Bolund and Nielsen, {Anders Lade} and Yonglun Luo",

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doi = "10.1093/gigascience/giy011",

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T1 - Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases

AU - Lin, Lin

AU - Liu, Yong

AU - Xu, Fengping

AU - Huang, Jinrong

AU - Daugaard, Tina Fuglsang

AU - Petersen, Trine Skov

AU - Hansen, Bettina

AU - Ye, Lingfei

AU - Zhou, Qing

AU - Fang, Fang

AU - Yang, Ling

AU - Li, Shengting

AU - Fløe, Lasse

AU - Jensen, Kristopher Torp

AU - Shrock, Ellen

AU - Chen, Fang

AU - Yang, Huanming

AU - Wang, Jian

AU - Liu, Xin

AU - Xu, Xun

AU - Bolund, Lars

AU - Nielsen, Anders Lade

AU - Luo, Yonglun

PY - 2018/3/1

Y1 - 2018/3/1

KW - DNA methylation

KW - CRISPR

KW - Cas9

KW - DNMT3A

KW - DNMT3B

KW - dCas9

KW - specificity

KW - off-targets

KW - epigenome editing

U2 - 10.1093/gigascience/giy011

DO - 10.1093/gigascience/giy011

M3 - Journal article

C2 - 29635374

SN - 2047-217X

VL - 7

JO - GigaScience

JF - GigaScience

IS - 3

ER -

Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases

Keywords

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