Genome sequences of the human body louse and its primary endosymbiont provide insights into the permanent parasitic lifestyle

Ewen F. Kirkness; Brian J. Haas; Weilin Sun; Henk R. Braig; M. Alejandra Perotti; John M. Clark; Si Hyeock Lee; Hugh M. Robertson; Ryan C. Kennedy; Eran Elhaik; Daniel Gerlach; Evgenia V. Kriventseva; Christine G. Elsik; Dan Graur; Catherine A. Hill; Jan A. Veenstra; Brian Walenz; José Manuel C Tubío; José M C Ribeiro; Julio Rozas; J. Spencer Johnston; Justin T. Reese; Aleksandar Popadic; Marta Tojo; Didier Raoult; David L. Reed; Yoshinori Tomoyasu; Emily Krause; Omprakash Mittapalli; Venu M. Margam; Hong Mei Li; Jason M. Meyer; Reed M. Johnson; Jeanne Romero-Severson; Janice Pagel VanZee; David Alvarez-Ponce; Filipe G. Vieira; Montserrat Aguadé; Sara Guirao-Rico; Juan M. Anzola; Kyong S. Yoon; Joseph P. Strycharz; Maria F. Unger; Scott Christley; Neil F. Lobo; Manfredo J. Seufferheld; Nai Kuan Wang; Gregory A. Dasch; Claudio J. Struchiner; Greg Madey; Linda I. Hannick; Shelby Bidwell; Vinita Joardar; Elisabet Caler; Renfu Shao; Stephen C. Barker; Stephen Cameron; Robert V. Bruggner; Allison Regier; Justin Johnson; Lakshmi Viswanathan; Terry R. Utterback; Granger G. Sutton; Daniel Lawson; Robert M. Waterhouse; J. Craig Venter; Robert L. Strausberg; May R. Berenbaum; Frank H. Collins; Evgeny M. Zdobnov; Barry R. Pittendrigh

doi:10.1073/pnas.1003379107

Genome sequences of the human body louse and its primary endosymbiont provide insights into the permanent parasitic lifestyle

Ewen F. Kirkness, Brian J. Haas, Weilin Sun, Henk R. Braig, M. Alejandra Perotti, John M. Clark, Si Hyeock Lee, Hugh M. Robertson, Ryan C. Kennedy, Eran Elhaik, Daniel Gerlach, Evgenia V. Kriventseva, Christine G. Elsik, Dan Graur, Catherine A. Hill, Jan A. Veenstra, Brian Walenz, José Manuel C Tubío, José M C Ribeiro, Julio RozasJ. Spencer Johnston, Justin T. Reese, Aleksandar Popadic, Marta Tojo, Didier Raoult, David L. Reed, Yoshinori Tomoyasu, Emily Krause, Omprakash Mittapalli, Venu M. Margam, Hong Mei Li, Jason M. Meyer, Reed M. Johnson, Jeanne Romero-Severson, Janice Pagel VanZee, David Alvarez-Ponce, Filipe G. Vieira, Montserrat Aguadé, Sara Guirao-Rico, Juan M. Anzola, Kyong S. Yoon, Joseph P. Strycharz, Maria F. Unger, Scott Christley, Neil F. Lobo, Manfredo J. Seufferheld, Nai Kuan Wang, Gregory A. Dasch, Claudio J. Struchiner, Greg Madey, Linda I. Hannick, Shelby Bidwell, Vinita Joardar, Elisabet Caler, Renfu Shao, Stephen C. Barker, Stephen Cameron, Robert V. Bruggner, Allison Regier, Justin Johnson, Lakshmi Viswanathan, Terry R. Utterback, Granger G. Sutton, Daniel Lawson, Robert M. Waterhouse, J. Craig Venter, Robert L. Strausberg, May R. Berenbaum, Frank H. Collins, Evgeny M. Zdobnov, Barry R. Pittendrigh

324 Citations (Scopus)

Abstract

As an obligatory parasite of humans, the body louse (Pediculus humanus humanus) is an important vector for human diseases, including epidemic typhus, relapsing fever, and trench fever. Here, we present genome sequences of the body louse and its primary bacterial endosymbiont Candidatus Riesia pediculicola. The body louse has the smallest known insect genome, spanning 108 Mb. Despite its status as an obligate parasite, it retains a remarkably complete basal insect repertoire of 10,773 protein-coding genes and 57 microRNAs. Representing hemimetabolous insects, the genome of the body louse thus provides a reference for studies of holometabolous insects. Compared with other insect genomes, the body louse genome contains significantly fewer genes associated with environmental sensing and response, including odorant and gustatory receptors and detoxifying enzymes. The unique architecture of the 18 minicircular mitochondrial chromosomes of the body louse may be linked to the loss of the gene encoding the mitochondrial single-stranded DNA binding protein. The genome of the obligatory louse endosymbiont Candidatus Riesia pediculicola encodes less than 600 genes on a short, linear chromosome and a circular plasmid. The plasmid harbors a unique arrangement of genes required for the synthesis of pantothenate, an essential vitamin deficient in the louse diet. The human body louse, its primary endosymbiont, and the bacterial pathogens that it vectors all possess genomes reduced in size compared with their free-living close relatives. Thus, the body louse genome project offers unique information and tools to use in advancing understanding of coevolution among vectors, symbionts, and pathogens.

Original language	English
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	107
Issue number	27
Pages (from-to)	12168-12173
Number of pages	6
ISSN	0027-8424
DOIs	https://doi.org/10.1073/pnas.1003379107
Publication status	Published - 6 Jul 2010
Externally published	Yes

Keywords

Coevolution
Comparative genomics
Ectoparasite

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Kirkness, E. F., Haas, B. J., Sun, W., Braig, H. R., Perotti, M. A., Clark, J. M., Lee, S. H., Robertson, H. M., Kennedy, R. C., Elhaik, E., Gerlach, D., Kriventseva, E. V., Elsik, C. G., Graur, D., Hill, C. A., Veenstra, J. A., Walenz, B., Tubío, J. M. C., Ribeiro, J. M. C., ... Pittendrigh, B. R. (2010). Genome sequences of the human body louse and its primary endosymbiont provide insights into the permanent parasitic lifestyle. Proceedings of the National Academy of Sciences of the United States of America, 107(27), 12168-12173. https://doi.org/10.1073/pnas.1003379107

Genome sequences of the human body louse and its primary endosymbiont provide insights into the permanent parasitic lifestyle. / Kirkness, Ewen F.; Haas, Brian J.; Sun, Weilin et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, No. 27, 06.07.2010, p. 12168-12173.

Research output: Contribution to journal › Journal article › Research › peer-review

Kirkness, EF, Haas, BJ, Sun, W, Braig, HR, Perotti, MA, Clark, JM, Lee, SH, Robertson, HM, Kennedy, RC, Elhaik, E, Gerlach, D, Kriventseva, EV, Elsik, CG, Graur, D, Hill, CA, Veenstra, JA, Walenz, B, Tubío, JMC, Ribeiro, JMC, Rozas, J, Johnston, JS, Reese, JT, Popadic, A, Tojo, M, Raoult, D, Reed, DL, Tomoyasu, Y, Krause, E, Mittapalli, O, Margam, VM, Li, HM, Meyer, JM, Johnson, RM, Romero-Severson, J, VanZee, JP, Alvarez-Ponce, D, Vieira, FG, Aguadé, M, Guirao-Rico, S, Anzola, JM, Yoon, KS, Strycharz, JP, Unger, MF, Christley, S, Lobo, NF, Seufferheld, MJ, Wang, NK, Dasch, GA, Struchiner, CJ, Madey, G, Hannick, LI, Bidwell, S, Joardar, V, Caler, E, Shao, R, Barker, SC, Cameron, S, Bruggner, RV, Regier, A, Johnson, J, Viswanathan, L, Utterback, TR, Sutton, GG, Lawson, D, Waterhouse, RM, Venter, JC, Strausberg, RL, Berenbaum, MR, Collins, FH, Zdobnov, EM & Pittendrigh, BR 2010, 'Genome sequences of the human body louse and its primary endosymbiont provide insights into the permanent parasitic lifestyle', Proceedings of the National Academy of Sciences of the United States of America, vol. 107, no. 27, pp. 12168-12173. https://doi.org/10.1073/pnas.1003379107

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abstract = "As an obligatory parasite of humans, the body louse (Pediculus humanus humanus) is an important vector for human diseases, including epidemic typhus, relapsing fever, and trench fever. Here, we present genome sequences of the body louse and its primary bacterial endosymbiont Candidatus Riesia pediculicola. The body louse has the smallest known insect genome, spanning 108 Mb. Despite its status as an obligate parasite, it retains a remarkably complete basal insect repertoire of 10,773 protein-coding genes and 57 microRNAs. Representing hemimetabolous insects, the genome of the body louse thus provides a reference for studies of holometabolous insects. Compared with other insect genomes, the body louse genome contains significantly fewer genes associated with environmental sensing and response, including odorant and gustatory receptors and detoxifying enzymes. The unique architecture of the 18 minicircular mitochondrial chromosomes of the body louse may be linked to the loss of the gene encoding the mitochondrial single-stranded DNA binding protein. The genome of the obligatory louse endosymbiont Candidatus Riesia pediculicola encodes less than 600 genes on a short, linear chromosome and a circular plasmid. The plasmid harbors a unique arrangement of genes required for the synthesis of pantothenate, an essential vitamin deficient in the louse diet. The human body louse, its primary endosymbiont, and the bacterial pathogens that it vectors all possess genomes reduced in size compared with their free-living close relatives. Thus, the body louse genome project offers unique information and tools to use in advancing understanding of coevolution among vectors, symbionts, and pathogens.",

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T1 - Genome sequences of the human body louse and its primary endosymbiont provide insights into the permanent parasitic lifestyle

AU - Kirkness, Ewen F.

AU - Haas, Brian J.

AU - Sun, Weilin

AU - Braig, Henk R.

AU - Perotti, M. Alejandra

AU - Clark, John M.

AU - Lee, Si Hyeock

AU - Robertson, Hugh M.

AU - Kennedy, Ryan C.

AU - Elhaik, Eran

AU - Gerlach, Daniel

AU - Kriventseva, Evgenia V.

AU - Elsik, Christine G.

AU - Graur, Dan

AU - Hill, Catherine A.

AU - Veenstra, Jan A.

AU - Walenz, Brian

AU - Tubío, José Manuel C

AU - Ribeiro, José M C

AU - Rozas, Julio

AU - Johnston, J. Spencer

AU - Reese, Justin T.

AU - Popadic, Aleksandar

AU - Tojo, Marta

AU - Raoult, Didier

AU - Reed, David L.

AU - Tomoyasu, Yoshinori

AU - Krause, Emily

AU - Mittapalli, Omprakash

AU - Margam, Venu M.

AU - Li, Hong Mei

AU - Meyer, Jason M.

AU - Johnson, Reed M.

AU - Romero-Severson, Jeanne

AU - VanZee, Janice Pagel

AU - Alvarez-Ponce, David

AU - Vieira, Filipe G.

AU - Aguadé, Montserrat

AU - Guirao-Rico, Sara

AU - Anzola, Juan M.

AU - Yoon, Kyong S.

AU - Strycharz, Joseph P.

AU - Unger, Maria F.

AU - Christley, Scott

AU - Lobo, Neil F.

AU - Seufferheld, Manfredo J.

AU - Wang, Nai Kuan

AU - Dasch, Gregory A.

AU - Struchiner, Claudio J.

AU - Madey, Greg

AU - Hannick, Linda I.

AU - Bidwell, Shelby

AU - Joardar, Vinita

AU - Caler, Elisabet

AU - Shao, Renfu

AU - Barker, Stephen C.

AU - Cameron, Stephen

AU - Bruggner, Robert V.

AU - Regier, Allison

AU - Johnson, Justin

AU - Viswanathan, Lakshmi

AU - Utterback, Terry R.

AU - Sutton, Granger G.

AU - Lawson, Daniel

AU - Waterhouse, Robert M.

AU - Venter, J. Craig

AU - Strausberg, Robert L.

AU - Berenbaum, May R.

AU - Collins, Frank H.

AU - Zdobnov, Evgeny M.

AU - Pittendrigh, Barry R.

PY - 2010/7/6

Y1 - 2010/7/6

N2 - As an obligatory parasite of humans, the body louse (Pediculus humanus humanus) is an important vector for human diseases, including epidemic typhus, relapsing fever, and trench fever. Here, we present genome sequences of the body louse and its primary bacterial endosymbiont Candidatus Riesia pediculicola. The body louse has the smallest known insect genome, spanning 108 Mb. Despite its status as an obligate parasite, it retains a remarkably complete basal insect repertoire of 10,773 protein-coding genes and 57 microRNAs. Representing hemimetabolous insects, the genome of the body louse thus provides a reference for studies of holometabolous insects. Compared with other insect genomes, the body louse genome contains significantly fewer genes associated with environmental sensing and response, including odorant and gustatory receptors and detoxifying enzymes. The unique architecture of the 18 minicircular mitochondrial chromosomes of the body louse may be linked to the loss of the gene encoding the mitochondrial single-stranded DNA binding protein. The genome of the obligatory louse endosymbiont Candidatus Riesia pediculicola encodes less than 600 genes on a short, linear chromosome and a circular plasmid. The plasmid harbors a unique arrangement of genes required for the synthesis of pantothenate, an essential vitamin deficient in the louse diet. The human body louse, its primary endosymbiont, and the bacterial pathogens that it vectors all possess genomes reduced in size compared with their free-living close relatives. Thus, the body louse genome project offers unique information and tools to use in advancing understanding of coevolution among vectors, symbionts, and pathogens.

AB - As an obligatory parasite of humans, the body louse (Pediculus humanus humanus) is an important vector for human diseases, including epidemic typhus, relapsing fever, and trench fever. Here, we present genome sequences of the body louse and its primary bacterial endosymbiont Candidatus Riesia pediculicola. The body louse has the smallest known insect genome, spanning 108 Mb. Despite its status as an obligate parasite, it retains a remarkably complete basal insect repertoire of 10,773 protein-coding genes and 57 microRNAs. Representing hemimetabolous insects, the genome of the body louse thus provides a reference for studies of holometabolous insects. Compared with other insect genomes, the body louse genome contains significantly fewer genes associated with environmental sensing and response, including odorant and gustatory receptors and detoxifying enzymes. The unique architecture of the 18 minicircular mitochondrial chromosomes of the body louse may be linked to the loss of the gene encoding the mitochondrial single-stranded DNA binding protein. The genome of the obligatory louse endosymbiont Candidatus Riesia pediculicola encodes less than 600 genes on a short, linear chromosome and a circular plasmid. The plasmid harbors a unique arrangement of genes required for the synthesis of pantothenate, an essential vitamin deficient in the louse diet. The human body louse, its primary endosymbiont, and the bacterial pathogens that it vectors all possess genomes reduced in size compared with their free-living close relatives. Thus, the body louse genome project offers unique information and tools to use in advancing understanding of coevolution among vectors, symbionts, and pathogens.

KW - Coevolution

KW - Comparative genomics

KW - Ectoparasite

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U2 - 10.1073/pnas.1003379107

DO - 10.1073/pnas.1003379107

M3 - Journal article

C2 - 20566863

AN - SCOPUS:77955459130

SN - 0027-8424

VL - 107

SP - 12168

EP - 12173

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 27

ER -

Genome sequences of the human body louse and its primary endosymbiont provide insights into the permanent parasitic lifestyle

Abstract

Keywords

UN SDGs

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