Genetic blueprint of the zoonotic pathogen Toxocara canis

Xing-Quan Zhu; Pasi K. Korhonen; Huimin Cai; Neil D. Young; Peter Nejsum; Georg von Samson-Himmelstjerna; Peter R. Boag; Patrick Tan; Qiye Li; Jiumeng Min; Yulan Yang; Xiuhua Wang; Xiaodong Fang; Ross S. Hall; Andreas Hofmann; Paul W. Sternberg; Aaron R. Jex; Robin B. Gasser

doi:10.1038/ncomms7145

Genetic blueprint of the zoonotic pathogen Toxocara canis

Xing-Quan Zhu, Pasi K. Korhonen, Huimin Cai, Neil D. Young, Peter Nejsum, Georg von Samson-Himmelstjerna, Peter R. Boag, Patrick Tan, Qiye Li, Jiumeng Min, Yulan Yang, Xiuhua Wang, Xiaodong Fang, Ross S. Hall, Andreas Hofmann, Paul W. Sternberg, Aaron R. Jex, Robin B. Gasser

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Abstract

Toxocara canis is a zoonotic parasite of major socioeconomic importance worldwide. In humans, this nematode causes disease (toxocariasis) mainly in the under-privileged communities in developed and developing countries. Although relatively well studied from clinical and epidemiological perspectives, to date, there has been no global investigation of the molecular biology of this parasite. Here we use next-generation sequencing to produce a draft genome and transcriptome of T. canis to support future biological and biotechnological investigations. This genome is 317 Mb in size, has a repeat content of 13.5% and encodes at least 18,596 protein-coding genes. We study transcription in a larval, as well as adult female and male stages, characterize the parasite's gene-silencing machinery, explore molecules involved in development or host-parasite interactions and predict intervention targets. The draft genome of T. canis should provide a useful resource for future molecular studies of this and other, related parasites.

Original language	English
Article number	6145
Journal	Nature Communications
Volume	6
Number of pages	8
ISSN	2041-1723
DOIs	https://doi.org/10.1038/ncomms7145
Publication status	Published - 4 Feb 2015

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title = "Genetic blueprint of the zoonotic pathogen Toxocara canis",

abstract = "Toxocara canis is a zoonotic parasite of major socioeconomic importance worldwide. In humans, this nematode causes disease (toxocariasis) mainly in the under-privileged communities in developed and developing countries. Although relatively well studied from clinical and epidemiological perspectives, to date, there has been no global investigation of the molecular biology of this parasite. Here we use next-generation sequencing to produce a draft genome and transcriptome of T. canis to support future biological and biotechnological investigations. This genome is 317 Mb in size, has a repeat content of 13.5% and encodes at least 18,596 protein-coding genes. We study transcription in a larval, as well as adult female and male stages, characterize the parasite's gene-silencing machinery, explore molecules involved in development or host-parasite interactions and predict intervention targets. The draft genome of T. canis should provide a useful resource for future molecular studies of this and other, related parasites.",

author = "Xing-Quan Zhu and Korhonen, {Pasi K.} and Huimin Cai and Young, {Neil D.} and Peter Nejsum and {von Samson-Himmelstjerna}, Georg and Boag, {Peter R.} and Patrick Tan and Qiye Li and Jiumeng Min and Yulan Yang and Xiuhua Wang and Xiaodong Fang and Hall, {Ross S.} and Andreas Hofmann and Sternberg, {Paul W.} and Jex, {Aaron R.} and Gasser, {Robin B.}",

year = "2015",

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day = "4",

doi = "10.1038/ncomms7145",

language = "English",

volume = "6",

journal = "Nature Communications",

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T1 - Genetic blueprint of the zoonotic pathogen Toxocara canis

AU - Zhu, Xing-Quan

AU - Korhonen, Pasi K.

AU - Cai, Huimin

AU - Young, Neil D.

AU - Nejsum, Peter

AU - von Samson-Himmelstjerna, Georg

AU - Boag, Peter R.

AU - Tan, Patrick

AU - Li, Qiye

AU - Min, Jiumeng

AU - Yang, Yulan

AU - Wang, Xiuhua

AU - Fang, Xiaodong

AU - Hall, Ross S.

AU - Hofmann, Andreas

AU - Sternberg, Paul W.

AU - Jex, Aaron R.

AU - Gasser, Robin B.

PY - 2015/2/4

Y1 - 2015/2/4

N2 - Toxocara canis is a zoonotic parasite of major socioeconomic importance worldwide. In humans, this nematode causes disease (toxocariasis) mainly in the under-privileged communities in developed and developing countries. Although relatively well studied from clinical and epidemiological perspectives, to date, there has been no global investigation of the molecular biology of this parasite. Here we use next-generation sequencing to produce a draft genome and transcriptome of T. canis to support future biological and biotechnological investigations. This genome is 317 Mb in size, has a repeat content of 13.5% and encodes at least 18,596 protein-coding genes. We study transcription in a larval, as well as adult female and male stages, characterize the parasite's gene-silencing machinery, explore molecules involved in development or host-parasite interactions and predict intervention targets. The draft genome of T. canis should provide a useful resource for future molecular studies of this and other, related parasites.

AB - Toxocara canis is a zoonotic parasite of major socioeconomic importance worldwide. In humans, this nematode causes disease (toxocariasis) mainly in the under-privileged communities in developed and developing countries. Although relatively well studied from clinical and epidemiological perspectives, to date, there has been no global investigation of the molecular biology of this parasite. Here we use next-generation sequencing to produce a draft genome and transcriptome of T. canis to support future biological and biotechnological investigations. This genome is 317 Mb in size, has a repeat content of 13.5% and encodes at least 18,596 protein-coding genes. We study transcription in a larval, as well as adult female and male stages, characterize the parasite's gene-silencing machinery, explore molecules involved in development or host-parasite interactions and predict intervention targets. The draft genome of T. canis should provide a useful resource for future molecular studies of this and other, related parasites.

U2 - 10.1038/ncomms7145

DO - 10.1038/ncomms7145

M3 - Journal article

C2 - 25649139

SN - 2041-1723

VL - 6

JO - Nature Communications

JF - Nature Communications

M1 - 6145

ER -

Genetic blueprint of the zoonotic pathogen Toxocara canis

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