Genetic and environmental influences on oxidative damage assessed in elderly Danish twins

Kasper Broedbaek; Rasmus Ribel-Madsen; Trine Henriksen; Allan Weimann; Morten Petersen; Jon T Andersen; Shoaib Afzal; Brian Hjelvang; L Jackson Roberts; Allan Vaag; Pernille Poulsen; Henrik E Poulsen

doi:10.1016/j.freeradbiomed.2011.02.017

Genetic and environmental influences on oxidative damage assessed in elderly Danish twins

Kasper Broedbaek, Rasmus Ribel-Madsen, Trine Henriksen, Allan Weimann, Morten Petersen, Jon T Andersen, Shoaib Afzal, Brian Hjelvang, L Jackson Roberts, Allan Vaag, Pernille Poulsen, Henrik E Poulsen

Department of Clinical Medicine

25 Citations (Scopus)

Abstract

Previous studies have shown an association between oxidative stress and various diseases in humans including cancer, cardiovascular disease, diabetes, and chronic respiratory disease. To what extents this damage is determined by genetic and environmental factors is unknown. In a classical twin study with 198 elderly twins we examined the contributions of genetic versus environmental factors to nucleic acid oxidation and lipid peroxidation. Urinary excretion of 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG), 8-oxo-7,8-dihydroguanosine (8-oxoGuo), and dinor,dihydro F₂-isoprostane metabolites (F ₂-IsoP-M) was measured using liquid chromatography-tandem mass spectrometry. The environmental influence on nucleic acid oxidation and lipid peroxidation was predominant, leaving only little influence from genetic factors, as evidenced by no differences in intraclass correlations between monozygotic (MZ) and dizygotic (DZ) twins, neither for 8-oxodG (r_MZ = 0.55, r_DZ = 0.47; P = 0.43), F₂-IsoP-M (r_MZ = 0.33, r_DZ = 0.22; P = 0.42), nor 8-oxoGuo (r_MZ = 0.45, r_DZ = 0.58; P = 0.21). Accordingly, heritability estimates for the three markers of oxidative damage were low (h² = 0.17-0.22). The three urinary markers of oxidative stress were closely correlated (r = 0.60-0.84). In conclusion, we demonstrated in a large population of elderly Danish twins that "whole-body" oxidative damage to nucleic acids and lipids is predominantly determined by potentially modifiable nongenetic factors.

Original language	English
Journal	Free Radical Biology & Medicine
Volume	50
Issue number	11
Pages (from-to)	1488-91
Number of pages	4
ISSN	0891-5849
DOIs	https://doi.org/10.1016/j.freeradbiomed.2011.02.017
Publication status	Published - 1 Jun 2011

Keywords

Aged
Aged, 80 and over
Biological Markers
DNA Damage
Denmark
Deoxyguanosine
Environmental Exposure
F2-Isoprostanes
Female
Guanosine
Humans
Lipid Peroxidation
Male
Middle Aged
Oxidation-Reduction
Oxidative Stress
Twins, Dizygotic
Twins, Monozygotic

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.freeradbiomed.2011.02.017

Cite this

Broedbaek, K., Ribel-Madsen, R., Henriksen, T., Weimann, A., Petersen, M., Andersen, J. T., Afzal, S., Hjelvang, B., Roberts, L. J., Vaag, A., Poulsen, P., & Poulsen, H. E. (2011). Genetic and environmental influences on oxidative damage assessed in elderly Danish twins. Free Radical Biology & Medicine, 50(11), 1488-91. https://doi.org/10.1016/j.freeradbiomed.2011.02.017

@article{07c616c13b324222abee6c62191be44c,

title = "Genetic and environmental influences on oxidative damage assessed in elderly Danish twins",

abstract = "Previous studies have shown an association between oxidative stress and various diseases in humans including cancer, cardiovascular disease, diabetes, and chronic respiratory disease. To what extents this damage is determined by genetic and environmental factors is unknown. In a classical twin study with 198 elderly twins we examined the contributions of genetic versus environmental factors to nucleic acid oxidation and lipid peroxidation. Urinary excretion of 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG), 8-oxo-7,8-dihydroguanosine (8-oxoGuo), and dinor,dihydro F2-isoprostane metabolites (F 2-IsoP-M) was measured using liquid chromatography-tandem mass spectrometry. The environmental influence on nucleic acid oxidation and lipid peroxidation was predominant, leaving only little influence from genetic factors, as evidenced by no differences in intraclass correlations between monozygotic (MZ) and dizygotic (DZ) twins, neither for 8-oxodG (rMZ = 0.55, rDZ = 0.47; P = 0.43), F2-IsoP-M (rMZ = 0.33, rDZ = 0.22; P = 0.42), nor 8-oxoGuo (rMZ = 0.45, rDZ = 0.58; P = 0.21). Accordingly, heritability estimates for the three markers of oxidative damage were low (h2 = 0.17-0.22). The three urinary markers of oxidative stress were closely correlated (r = 0.60-0.84). In conclusion, we demonstrated in a large population of elderly Danish twins that {"}whole-body{"} oxidative damage to nucleic acids and lipids is predominantly determined by potentially modifiable nongenetic factors.",

keywords = "Aged, Aged, 80 and over, Biological Markers, DNA Damage, Denmark, Deoxyguanosine, Environmental Exposure, F2-Isoprostanes, Female, Guanosine, Humans, Lipid Peroxidation, Male, Middle Aged, Oxidation-Reduction, Oxidative Stress, Twins, Dizygotic, Twins, Monozygotic",

author = "Kasper Broedbaek and Rasmus Ribel-Madsen and Trine Henriksen and Allan Weimann and Morten Petersen and Andersen, {Jon T} and Shoaib Afzal and Brian Hjelvang and Roberts, {L Jackson} and Allan Vaag and Pernille Poulsen and Poulsen, {Henrik E}",

year = "2011",

month = jun,

day = "1",

doi = "10.1016/j.freeradbiomed.2011.02.017",

language = "English",

volume = "50",

pages = "1488--91",

journal = "Free Radical Biology & Medicine",

issn = "0891-5849",

publisher = "Elsevier",

number = "11",

}

TY - JOUR

T1 - Genetic and environmental influences on oxidative damage assessed in elderly Danish twins

AU - Broedbaek, Kasper

AU - Ribel-Madsen, Rasmus

AU - Henriksen, Trine

AU - Weimann, Allan

AU - Petersen, Morten

AU - Andersen, Jon T

AU - Afzal, Shoaib

AU - Hjelvang, Brian

AU - Roberts, L Jackson

AU - Vaag, Allan

AU - Poulsen, Pernille

AU - Poulsen, Henrik E

PY - 2011/6/1

Y1 - 2011/6/1

N2 - Previous studies have shown an association between oxidative stress and various diseases in humans including cancer, cardiovascular disease, diabetes, and chronic respiratory disease. To what extents this damage is determined by genetic and environmental factors is unknown. In a classical twin study with 198 elderly twins we examined the contributions of genetic versus environmental factors to nucleic acid oxidation and lipid peroxidation. Urinary excretion of 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG), 8-oxo-7,8-dihydroguanosine (8-oxoGuo), and dinor,dihydro F2-isoprostane metabolites (F 2-IsoP-M) was measured using liquid chromatography-tandem mass spectrometry. The environmental influence on nucleic acid oxidation and lipid peroxidation was predominant, leaving only little influence from genetic factors, as evidenced by no differences in intraclass correlations between monozygotic (MZ) and dizygotic (DZ) twins, neither for 8-oxodG (rMZ = 0.55, rDZ = 0.47; P = 0.43), F2-IsoP-M (rMZ = 0.33, rDZ = 0.22; P = 0.42), nor 8-oxoGuo (rMZ = 0.45, rDZ = 0.58; P = 0.21). Accordingly, heritability estimates for the three markers of oxidative damage were low (h2 = 0.17-0.22). The three urinary markers of oxidative stress were closely correlated (r = 0.60-0.84). In conclusion, we demonstrated in a large population of elderly Danish twins that "whole-body" oxidative damage to nucleic acids and lipids is predominantly determined by potentially modifiable nongenetic factors.

AB - Previous studies have shown an association between oxidative stress and various diseases in humans including cancer, cardiovascular disease, diabetes, and chronic respiratory disease. To what extents this damage is determined by genetic and environmental factors is unknown. In a classical twin study with 198 elderly twins we examined the contributions of genetic versus environmental factors to nucleic acid oxidation and lipid peroxidation. Urinary excretion of 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG), 8-oxo-7,8-dihydroguanosine (8-oxoGuo), and dinor,dihydro F2-isoprostane metabolites (F 2-IsoP-M) was measured using liquid chromatography-tandem mass spectrometry. The environmental influence on nucleic acid oxidation and lipid peroxidation was predominant, leaving only little influence from genetic factors, as evidenced by no differences in intraclass correlations between monozygotic (MZ) and dizygotic (DZ) twins, neither for 8-oxodG (rMZ = 0.55, rDZ = 0.47; P = 0.43), F2-IsoP-M (rMZ = 0.33, rDZ = 0.22; P = 0.42), nor 8-oxoGuo (rMZ = 0.45, rDZ = 0.58; P = 0.21). Accordingly, heritability estimates for the three markers of oxidative damage were low (h2 = 0.17-0.22). The three urinary markers of oxidative stress were closely correlated (r = 0.60-0.84). In conclusion, we demonstrated in a large population of elderly Danish twins that "whole-body" oxidative damage to nucleic acids and lipids is predominantly determined by potentially modifiable nongenetic factors.

KW - Aged

KW - Aged, 80 and over

KW - Biological Markers

KW - DNA Damage

KW - Denmark

KW - Deoxyguanosine

KW - Environmental Exposure

KW - F2-Isoprostanes

KW - Female

KW - Guanosine

KW - Humans

KW - Lipid Peroxidation

KW - Male

KW - Middle Aged

KW - Oxidation-Reduction

KW - Oxidative Stress

KW - Twins, Dizygotic

KW - Twins, Monozygotic

U2 - 10.1016/j.freeradbiomed.2011.02.017

DO - 10.1016/j.freeradbiomed.2011.02.017

M3 - Journal article

C2 - 21354303

SN - 0891-5849

VL - 50

SP - 1488

EP - 1491

JO - Free Radical Biology & Medicine

JF - Free Radical Biology & Medicine

IS - 11

ER -

Genetic and environmental influences on oxidative damage assessed in elderly Danish twins

Abstract

Keywords

UN SDGs

Access to Document

Fingerprint

Cite this