Genetic analyses in the hyperthermophilic archaeon Sulfolobus islandicus

TY - JOUR

T1 - Genetic analyses in the hyperthermophilic archaeon Sulfolobus islandicus

AU - She, Qunxin

AU - Zhang, Changyi

AU - Deng, Ling

AU - Peng, Nan

AU - Chen, Zhengjun

AU - Liang, Yun Xiang

N1 - Keywords: Gene Knockout Techniques; Genes, Reporter; Mutation; Promoter Regions, Genetic; Selection (Genetics); Sulfolobus

PY - 2009

Y1 - 2009

N2 - Sulfolobus belongs to the hyperthermophilic archaea and it serves as a model organism to study archaeal molecular biology and evolution. In the last few years, we have focused on developing genetic systems for Sulfolobus islandicus using pyrEF as a selection marker and versatile genetic tools have been developed, including methods for constructing gene knockouts and for identifying essential genes. These genetic tools enable us to conduct genetic analysis on the functions of the genes involved in DNA replication and repair processes in S. islandicus and they should also facilitate in vivo analysis of functions of other genes in this model organism.

AB - Sulfolobus belongs to the hyperthermophilic archaea and it serves as a model organism to study archaeal molecular biology and evolution. In the last few years, we have focused on developing genetic systems for Sulfolobus islandicus using pyrEF as a selection marker and versatile genetic tools have been developed, including methods for constructing gene knockouts and for identifying essential genes. These genetic tools enable us to conduct genetic analysis on the functions of the genes involved in DNA replication and repair processes in S. islandicus and they should also facilitate in vivo analysis of functions of other genes in this model organism.

U2 - 10.1042/BST0370092

DO - 10.1042/BST0370092

M3 - Journal article

C2 - 19143609

SN - 0300-5127

VL - 37

SP - 92

EP - 96

JO - Biochemical Society Transactions

JF - Biochemical Society Transactions

IS - Pt 1

ER -