Gene expression profiles of mouse spermatogenesis during recovery from irradiation

Fozia J Shah; Masami Tanaka; John E Nielsen; Teruaki Iwamoto; Shinichi Kobayashi; Niels E Skakkebaek; Henrik Leffers; Kristian Almstrup; Fozia J Shah; Masami Tanaka; John E Nielsen; Teruaki Iwamoto; Shinichi Kobayashi; Niels E Skakkebaek; Henrik Leffers; Kristian Almstrup

doi:10.1186/1477-7827-7-130

Gene expression profiles of mouse spermatogenesis during recovery from irradiation

Fozia J Shah, Masami Tanaka, John E Nielsen, Teruaki Iwamoto, Shinichi Kobayashi, Niels E Skakkebaek, Henrik Leffers, Kristian Almstrup, Fozia J Shah, Masami Tanaka, John E Nielsen, Teruaki Iwamoto, Shinichi Kobayashi, Niels E Skakkebaek, Henrik Leffers, Kristian Almstrup

14 Citations (Scopus)

Abstract

BACKGROUND: Irradiation or chemotherapy that suspend normal spermatogenesis is commonly used to treat various cancers. Fortunately, spermatogenesis in many cases can be restored after such treatments but knowledge is limited about the re-initiation process. Earlier studies have described the cellular changes that happen during recovery from irradiation by means of histology. We have earlier generated gene expression profiles during induction of spermatogenesis in mouse postnatal developing testes and found a correlation between profiles and the expressing cell types. The aim of the present work was to utilize the link between expression profile and cell types to follow the cellular changes that occur during post-irradiation recovery of spermatogenesis in order to describe recovery by means of gene expression. METHODS: Adult mouse testes were subjected to irradiation with 1 Gy or a fractionated radiation of two times 1 Gy. Testes were sampled every third or fourth day to follow the recovery of spermatogenesis and gene expression profiles generated by means of differential display RT-PCR. In situ hybridization was in addition performed to verify cell-type specific gene expression patterns. RESULTS: Irradiation of mice testis created a gap in spermatogenesis, which was initiated by loss of A1 to B-spermatogonia and lasted for approximately 10 days. Irradiation with 2 times 1 Gy showed a more pronounced effect on germ cell elimination than with 1 Gy, but spermatogenesis was in both cases completely reconstituted 42 days after irradiation. Comparison of expression profiles indicated that the cellular reconstitution appeared equivalent to what is observed during induction of normal spermatogenesis. CONCLUSION: The data indicates that recovery of spermatogenesis can be monitored by means of gene expression, which could aid in designing radiation treatment regimes for cancer patients leading to better restoration of spermatogenesis.

Original language	English
Journal	Reproductive Biology and Endocrinology
Volume	7
Pages (from-to)	130
ISSN	1477-7827
DOIs	https://doi.org/10.1186/1477-7827-7-130 https://doi.org/10.1186/1477-7827-7-130
Publication status	Published - 1 Jan 2009

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Shah, F. J., Tanaka, M., Nielsen, J. E., Iwamoto, T., Kobayashi, S., Skakkebaek, N. E., Leffers, H., Almstrup, K., Shah, F. J., Tanaka, M., Nielsen, J. E., Iwamoto, T., Kobayashi, S., Skakkebaek, N. E., Leffers, H., & Almstrup, K. (2009). Gene expression profiles of mouse spermatogenesis during recovery from irradiation. Reproductive Biology and Endocrinology, 7, 130. https://doi.org/10.1186/1477-7827-7-130, https://doi.org/10.1186/1477-7827-7-130

Shah, FJ, Tanaka, M, Nielsen, JE, Iwamoto, T, Kobayashi, S, Skakkebaek, NE, Leffers, H, Almstrup, K, Shah, FJ, Tanaka, M, Nielsen, JE, Iwamoto, T, Kobayashi, S, Skakkebaek, NE, Leffers, H & Almstrup, K 2009, 'Gene expression profiles of mouse spermatogenesis during recovery from irradiation', Reproductive Biology and Endocrinology, vol. 7, pp. 130. https://doi.org/10.1186/1477-7827-7-130, https://doi.org/10.1186/1477-7827-7-130

@article{ba34576068a111df928f000ea68e967b,

title = "Gene expression profiles of mouse spermatogenesis during recovery from irradiation",

abstract = "BACKGROUND: Irradiation or chemotherapy that suspend normal spermatogenesis is commonly used to treat various cancers. Fortunately, spermatogenesis in many cases can be restored after such treatments but knowledge is limited about the re-initiation process. Earlier studies have described the cellular changes that happen during recovery from irradiation by means of histology. We have earlier generated gene expression profiles during induction of spermatogenesis in mouse postnatal developing testes and found a correlation between profiles and the expressing cell types. The aim of the present work was to utilize the link between expression profile and cell types to follow the cellular changes that occur during post-irradiation recovery of spermatogenesis in order to describe recovery by means of gene expression. METHODS: Adult mouse testes were subjected to irradiation with 1 Gy or a fractionated radiation of two times 1 Gy. Testes were sampled every third or fourth day to follow the recovery of spermatogenesis and gene expression profiles generated by means of differential display RT-PCR. In situ hybridization was in addition performed to verify cell-type specific gene expression patterns. RESULTS: Irradiation of mice testis created a gap in spermatogenesis, which was initiated by loss of A1 to B-spermatogonia and lasted for approximately 10 days. Irradiation with 2 times 1 Gy showed a more pronounced effect on germ cell elimination than with 1 Gy, but spermatogenesis was in both cases completely reconstituted 42 days after irradiation. Comparison of expression profiles indicated that the cellular reconstitution appeared equivalent to what is observed during induction of normal spermatogenesis. CONCLUSION: The data indicates that recovery of spermatogenesis can be monitored by means of gene expression, which could aid in designing radiation treatment regimes for cancer patients leading to better restoration of spermatogenesis.",

author = "Shah, {Fozia J} and Masami Tanaka and Nielsen, {John E} and Teruaki Iwamoto and Shinichi Kobayashi and Skakkebaek, {Niels E} and Henrik Leffers and Kristian Almstrup and Shah, {Fozia J} and Masami Tanaka and Nielsen, {John E} and Teruaki Iwamoto and Shinichi Kobayashi and Skakkebaek, {Niels E} and Henrik Leffers and Kristian Almstrup",

note = "Keywords: Animals; Body Weight; GATA1 Transcription Factor; Gene Expression Profiling; Male; Mice; Mice, Inbred C3H; Nuclear Proteins; Organ Size; Organ Specificity; RNA-Binding Proteins; Recovery of Function; Spermatogenesis; Spermatogonia; Testis; Vesicular Transport Proteins; Whole-Body Irradiation",

year = "2009",

month = jan,

day = "1",

doi = "10.1186/1477-7827-7-130",

language = "English",

volume = "7",

pages = "130",

journal = "Reproductive Biology and Endocrinology",

issn = "1477-7827",

publisher = "BioMed Central",

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TY - JOUR

T1 - Gene expression profiles of mouse spermatogenesis during recovery from irradiation

AU - Shah, Fozia J

AU - Tanaka, Masami

AU - Nielsen, John E

AU - Iwamoto, Teruaki

AU - Kobayashi, Shinichi

AU - Skakkebaek, Niels E

AU - Leffers, Henrik

AU - Almstrup, Kristian

AU - Shah, Fozia J

AU - Tanaka, Masami

AU - Nielsen, John E

AU - Iwamoto, Teruaki

AU - Kobayashi, Shinichi

AU - Skakkebaek, Niels E

AU - Leffers, Henrik

AU - Almstrup, Kristian

N1 - Keywords: Animals; Body Weight; GATA1 Transcription Factor; Gene Expression Profiling; Male; Mice; Mice, Inbred C3H; Nuclear Proteins; Organ Size; Organ Specificity; RNA-Binding Proteins; Recovery of Function; Spermatogenesis; Spermatogonia; Testis; Vesicular Transport Proteins; Whole-Body Irradiation

PY - 2009/1/1

Y1 - 2009/1/1

N2 - BACKGROUND: Irradiation or chemotherapy that suspend normal spermatogenesis is commonly used to treat various cancers. Fortunately, spermatogenesis in many cases can be restored after such treatments but knowledge is limited about the re-initiation process. Earlier studies have described the cellular changes that happen during recovery from irradiation by means of histology. We have earlier generated gene expression profiles during induction of spermatogenesis in mouse postnatal developing testes and found a correlation between profiles and the expressing cell types. The aim of the present work was to utilize the link between expression profile and cell types to follow the cellular changes that occur during post-irradiation recovery of spermatogenesis in order to describe recovery by means of gene expression. METHODS: Adult mouse testes were subjected to irradiation with 1 Gy or a fractionated radiation of two times 1 Gy. Testes were sampled every third or fourth day to follow the recovery of spermatogenesis and gene expression profiles generated by means of differential display RT-PCR. In situ hybridization was in addition performed to verify cell-type specific gene expression patterns. RESULTS: Irradiation of mice testis created a gap in spermatogenesis, which was initiated by loss of A1 to B-spermatogonia and lasted for approximately 10 days. Irradiation with 2 times 1 Gy showed a more pronounced effect on germ cell elimination than with 1 Gy, but spermatogenesis was in both cases completely reconstituted 42 days after irradiation. Comparison of expression profiles indicated that the cellular reconstitution appeared equivalent to what is observed during induction of normal spermatogenesis. CONCLUSION: The data indicates that recovery of spermatogenesis can be monitored by means of gene expression, which could aid in designing radiation treatment regimes for cancer patients leading to better restoration of spermatogenesis.

AB - BACKGROUND: Irradiation or chemotherapy that suspend normal spermatogenesis is commonly used to treat various cancers. Fortunately, spermatogenesis in many cases can be restored after such treatments but knowledge is limited about the re-initiation process. Earlier studies have described the cellular changes that happen during recovery from irradiation by means of histology. We have earlier generated gene expression profiles during induction of spermatogenesis in mouse postnatal developing testes and found a correlation between profiles and the expressing cell types. The aim of the present work was to utilize the link between expression profile and cell types to follow the cellular changes that occur during post-irradiation recovery of spermatogenesis in order to describe recovery by means of gene expression. METHODS: Adult mouse testes were subjected to irradiation with 1 Gy or a fractionated radiation of two times 1 Gy. Testes were sampled every third or fourth day to follow the recovery of spermatogenesis and gene expression profiles generated by means of differential display RT-PCR. In situ hybridization was in addition performed to verify cell-type specific gene expression patterns. RESULTS: Irradiation of mice testis created a gap in spermatogenesis, which was initiated by loss of A1 to B-spermatogonia and lasted for approximately 10 days. Irradiation with 2 times 1 Gy showed a more pronounced effect on germ cell elimination than with 1 Gy, but spermatogenesis was in both cases completely reconstituted 42 days after irradiation. Comparison of expression profiles indicated that the cellular reconstitution appeared equivalent to what is observed during induction of normal spermatogenesis. CONCLUSION: The data indicates that recovery of spermatogenesis can be monitored by means of gene expression, which could aid in designing radiation treatment regimes for cancer patients leading to better restoration of spermatogenesis.

U2 - 10.1186/1477-7827-7-130

DO - 10.1186/1477-7827-7-130

M3 - Journal article

C2 - 19925657

SN - 1477-7827

VL - 7

SP - 130

JO - Reproductive Biology and Endocrinology

JF - Reproductive Biology and Endocrinology

ER -

Gene expression profiles of mouse spermatogenesis during recovery from irradiation

Abstract

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