TY - CHAP
T1 - Gene Expression Analysis Using Agilent DNA Microarrays
AU - Stangegaard, Michael
N1 - ikke relevant
PY - 2009
Y1 - 2009
N2 - Hybridization of labeled cDNA to microarrays is an intuitively simple and a vastly underestimated process. If it is not performed, optimized, and standardized with the same attention to detail as e.g., RNA amplification, information may be overlooked or even lost. Careful balancing of the amount of labeled cDNA added to each slide reduces dye-bias and slide to slide variation. Efficient mixing of the hybridization solution throughout the hybridization reaction increases signals several fold. The amount of near perfect target-probe hybrids may be reduced by efficient stringency washes of the hybridized microarray slides.
AB - Hybridization of labeled cDNA to microarrays is an intuitively simple and a vastly underestimated process. If it is not performed, optimized, and standardized with the same attention to detail as e.g., RNA amplification, information may be overlooked or even lost. Careful balancing of the amount of labeled cDNA added to each slide reduces dye-bias and slide to slide variation. Efficient mixing of the hybridization solution throughout the hybridization reaction increases signals several fold. The amount of near perfect target-probe hybrids may be reduced by efficient stringency washes of the hybridized microarray slides.
KW - Faculty of Health and Medical Sciences
U2 - 10.1007/978-1-59745-538-1_9
DO - 10.1007/978-1-59745-538-1_9
M3 - Book chapter
C2 - 19381965
SN - 1064-3745
SN - 978-1-934115-69-5
VL - 529
T3 - Methods in Molecular Biology
SP - 133
EP - 145
BT - DNA Microarrays for Biomedical Research
A2 - Dufva, Hans Martin
PB - Springer Science+Business Media
CY - Spring Street, New York, NY 10013, USA
ER -