Further development of a method for visualisation of exopolysaccharides in yoghurt using fluorescent conjugates

Lanjun Zhang; Ditte Marie Folkenberg; Karsten Bruun Qvist; Richard Ipsen

doi:10.1016/j.idairyj.2014.08.018

Further development of a method for visualisation of exopolysaccharides in yoghurt using fluorescent conjugates

Lanjun Zhang, Ditte Marie Folkenberg, Karsten Bruun Qvist, Richard Ipsen

Dairy, Meat and Plant Product Technology

10 Citations (Scopus)

Abstract

Exopolysaccharides (EPS) play a central role in creating yoghurt with high sensory appeal. To understand more precisely how EPS affect the microstructure of yoghurt, it is crucial to be able to visualise how EPS is distributed in the protein gel. Hence, the present study used confocal laser scanning microscopy combined with multiple fluorescent lectin conjugates in a single sample to further develop a methodology for visualising different types of EPS in acidified milk. Use of phosphate-buffered saline supplemented with CaCl₂ and MgCl₂ in the working solution of lectin conjugates minimised the influence of pH on their binding affinity to EPS in yoghurt. EPS could be visualised simultaneously by using lectin conjugates of concanavalin A and wheat germ agglutinin together. The method was verified using yoghurt samples made with commercial starter culture and can aid in the choice of starter cultures and processing parameters for yoghurt production.

Original language	English
Journal	International Dairy Journal
Volume	46
Pages (from-to)	88-95
Number of pages	8
ISSN	0958-6946
DOIs	https://doi.org/10.1016/j.idairyj.2014.08.018
Publication status	Published - 1 Jul 2015

Access to Document

10.1016/j.idairyj.2014.08.018

Cite this

@article{2bc5e63e2d1f456e9fab1503d07e9323,

title = "Further development of a method for visualisation of exopolysaccharides in yoghurt using fluorescent conjugates",

abstract = "Exopolysaccharides (EPS) play a central role in creating yoghurt with high sensory appeal. To understand more precisely how EPS affect the microstructure of yoghurt, it is crucial to be able to visualise how EPS is distributed in the protein gel. Hence, the present study used confocal laser scanning microscopy combined with multiple fluorescent lectin conjugates in a single sample to further develop a methodology for visualising different types of EPS in acidified milk. Use of phosphate-buffered saline supplemented with CaCl2 and MgCl2 in the working solution of lectin conjugates minimised the influence of pH on their binding affinity to EPS in yoghurt. EPS could be visualised simultaneously by using lectin conjugates of concanavalin A and wheat germ agglutinin together. The method was verified using yoghurt samples made with commercial starter culture and can aid in the choice of starter cultures and processing parameters for yoghurt production.",

author = "Lanjun Zhang and Folkenberg, {Ditte Marie} and Qvist, {Karsten Bruun} and Richard Ipsen",

note = "IDF Symposia on Microstructure of Dairy Products and Science and Technology of Fermented Milk, Melbourne, 2014",

year = "2015",

month = jul,

day = "1",

doi = "10.1016/j.idairyj.2014.08.018",

language = "English",

volume = "46",

pages = "88--95",

journal = "International Dairy Journal",

issn = "0958-6946",

publisher = "Elsevier",

}

TY - JOUR

T1 - Further development of a method for visualisation of exopolysaccharides in yoghurt using fluorescent conjugates

AU - Zhang, Lanjun

AU - Folkenberg, Ditte Marie

AU - Qvist, Karsten Bruun

AU - Ipsen, Richard

N1 - IDF Symposia on Microstructure of Dairy Products and Science and Technology of Fermented Milk, Melbourne, 2014

PY - 2015/7/1

Y1 - 2015/7/1

N2 - Exopolysaccharides (EPS) play a central role in creating yoghurt with high sensory appeal. To understand more precisely how EPS affect the microstructure of yoghurt, it is crucial to be able to visualise how EPS is distributed in the protein gel. Hence, the present study used confocal laser scanning microscopy combined with multiple fluorescent lectin conjugates in a single sample to further develop a methodology for visualising different types of EPS in acidified milk. Use of phosphate-buffered saline supplemented with CaCl2 and MgCl2 in the working solution of lectin conjugates minimised the influence of pH on their binding affinity to EPS in yoghurt. EPS could be visualised simultaneously by using lectin conjugates of concanavalin A and wheat germ agglutinin together. The method was verified using yoghurt samples made with commercial starter culture and can aid in the choice of starter cultures and processing parameters for yoghurt production.

AB - Exopolysaccharides (EPS) play a central role in creating yoghurt with high sensory appeal. To understand more precisely how EPS affect the microstructure of yoghurt, it is crucial to be able to visualise how EPS is distributed in the protein gel. Hence, the present study used confocal laser scanning microscopy combined with multiple fluorescent lectin conjugates in a single sample to further develop a methodology for visualising different types of EPS in acidified milk. Use of phosphate-buffered saline supplemented with CaCl2 and MgCl2 in the working solution of lectin conjugates minimised the influence of pH on their binding affinity to EPS in yoghurt. EPS could be visualised simultaneously by using lectin conjugates of concanavalin A and wheat germ agglutinin together. The method was verified using yoghurt samples made with commercial starter culture and can aid in the choice of starter cultures and processing parameters for yoghurt production.

U2 - 10.1016/j.idairyj.2014.08.018

DO - 10.1016/j.idairyj.2014.08.018

M3 - Journal article

SN - 0958-6946

VL - 46

SP - 88

EP - 95

JO - International Dairy Journal

JF - International Dairy Journal

ER -

Further development of a method for visualisation of exopolysaccharides in yoghurt using fluorescent conjugates

Abstract

Access to Document

Fingerprint

Cite this