Functional and molecular characterization of prostaglandin E2 dilatory receptors in the rat craniovascular system in relevance to migraine

Maja Myren; Michael Baun; Kenneth Beri Ploug; Inger Jansen-Olesen; Jes Olesen; Saurabh Gupta

doi:10.1177/0333102409357957

Functional and molecular characterization of prostaglandin E2 dilatory receptors in the rat craniovascular system in relevance to migraine

Maja Myren, Michael Baun, Kenneth Beri Ploug, Inger Jansen-Olesen, Jes Olesen, Saurabh Gupta

17 Citations (Scopus)

Abstract

Introduction: Migraine pain is thought to involve an increase in trigeminal nerve terminal activity around large cerebral and meningeal arteries, leading to vasodilatation. Because prostaglandin E₂ (PGE₂) is elevated in cephalic venous blood during migraine attacks, and is also capable of inducing headache in healthy volunteers, we hypothesize that PGE₂ dilatory receptors, EP₂ and EP₄, mediate the response. Materials and methods: By the use of specific agonists and antagonists, the dilatory effect of PGE₂ was characterized in rat cranial arteries by use of in vivo and in vitro methods. Furthermore, EP₂ and EP ₄ quantitative messenger RNA (mRNA) receptor expression was studied in the rat craniovascular system. Results: Our results suggest that EP ₄, and to a lesser degree EP₂, receptors mediate the dilatory effect of PGE₂ in the craniovascular system in rats. Thus, antagonism of these receptors might be of therapeutic relevance in migraine.

Original language	English
Journal	Cephalalgia
Volume	30
Issue number	9
Pages (from-to)	1110-22
Number of pages	13
ISSN	0333-1024
DOIs	https://doi.org/10.1177/0333102409357957
Publication status	Published - 1 Sept 2010

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title = "Functional and molecular characterization of prostaglandin E2 dilatory receptors in the rat craniovascular system in relevance to migraine",

abstract = "Introduction: Migraine pain is thought to involve an increase in trigeminal nerve terminal activity around large cerebral and meningeal arteries, leading to vasodilatation. Because prostaglandin E2 (PGE2) is elevated in cephalic venous blood during migraine attacks, and is also capable of inducing headache in healthy volunteers, we hypothesize that PGE2 dilatory receptors, EP2 and EP4, mediate the response. Materials and methods: By the use of specific agonists and antagonists, the dilatory effect of PGE2 was characterized in rat cranial arteries by use of in vivo and in vitro methods. Furthermore, EP2 and EP 4 quantitative messenger RNA (mRNA) receptor expression was studied in the rat craniovascular system. Results: Our results suggest that EP 4, and to a lesser degree EP2, receptors mediate the dilatory effect of PGE2 in the craniovascular system in rats. Thus, antagonism of these receptors might be of therapeutic relevance in migraine.",

author = "Maja Myren and Michael Baun and Ploug, {Kenneth Beri} and Inger Jansen-Olesen and Jes Olesen and Saurabh Gupta",

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doi = "10.1177/0333102409357957",

language = "English",

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journal = "Cephalalgia",

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TY - JOUR

T1 - Functional and molecular characterization of prostaglandin E2 dilatory receptors in the rat craniovascular system in relevance to migraine

AU - Myren, Maja

AU - Baun, Michael

AU - Ploug, Kenneth Beri

AU - Jansen-Olesen, Inger

AU - Olesen, Jes

AU - Gupta, Saurabh

PY - 2010/9/1

Y1 - 2010/9/1

N2 - Introduction: Migraine pain is thought to involve an increase in trigeminal nerve terminal activity around large cerebral and meningeal arteries, leading to vasodilatation. Because prostaglandin E2 (PGE2) is elevated in cephalic venous blood during migraine attacks, and is also capable of inducing headache in healthy volunteers, we hypothesize that PGE2 dilatory receptors, EP2 and EP4, mediate the response. Materials and methods: By the use of specific agonists and antagonists, the dilatory effect of PGE2 was characterized in rat cranial arteries by use of in vivo and in vitro methods. Furthermore, EP2 and EP 4 quantitative messenger RNA (mRNA) receptor expression was studied in the rat craniovascular system. Results: Our results suggest that EP 4, and to a lesser degree EP2, receptors mediate the dilatory effect of PGE2 in the craniovascular system in rats. Thus, antagonism of these receptors might be of therapeutic relevance in migraine.

AB - Introduction: Migraine pain is thought to involve an increase in trigeminal nerve terminal activity around large cerebral and meningeal arteries, leading to vasodilatation. Because prostaglandin E2 (PGE2) is elevated in cephalic venous blood during migraine attacks, and is also capable of inducing headache in healthy volunteers, we hypothesize that PGE2 dilatory receptors, EP2 and EP4, mediate the response. Materials and methods: By the use of specific agonists and antagonists, the dilatory effect of PGE2 was characterized in rat cranial arteries by use of in vivo and in vitro methods. Furthermore, EP2 and EP 4 quantitative messenger RNA (mRNA) receptor expression was studied in the rat craniovascular system. Results: Our results suggest that EP 4, and to a lesser degree EP2, receptors mediate the dilatory effect of PGE2 in the craniovascular system in rats. Thus, antagonism of these receptors might be of therapeutic relevance in migraine.

U2 - 10.1177/0333102409357957

DO - 10.1177/0333102409357957

M3 - Journal article

SN - 0333-1024

VL - 30

SP - 1110

EP - 1122

JO - Cephalalgia

JF - Cephalalgia

IS - 9

ER -

Functional and molecular characterization of prostaglandin E2 dilatory receptors in the rat craniovascular system in relevance to migraine

Abstract

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