TY - JOUR
T1 - Freeze-tolerance of Trichinella muscle larvae in experimentally infected wild boars
AU - Lacour, Sandrine A.
AU - Heckmann, Aurelie
AU - Mace, Pauline
AU - Grasset-Chevillot, Aurelie
AU - Zanella, Gina
AU - Vallee, Isabelle
AU - Kapel, Christian Moliin Outzen
AU - Boireau, Pascal
N1 - Special Issue: 13th International Conference on Trichinellosis
PY - 2013
Y1 - 2013
N2 - Freeze-tolerance of encapsulated Trichinella muscle larvae (ML) is mainly determined by Trichinella species, but is also influenced by host species, the age of the infection and the storage time and temperature of the infected meat. Moreover, the freeze-tolerance of the encapsulated species appears to be correlated to the development of thick capsule walls which increases with age. An extended infection period and the muscle composition in some hosts (e.g. herbivores) may provide freeze-avoiding matrices due to high carbohydrate contents. The present experiment compares freeze-tolerance of Trichinella spiralis and Trichinella britovi ML in wild boar meat 24 weeks post inoculation (wpi). Three groups of four wild boars were infected with 200, 2000 or 20,000 ML of T. britovi (ISS 1575), respectively. Additionally, three wild boars were inoculated with 20,000 ML of T. spiralis (ISS 004) and two animals served as negative controls. All wild boars were sacrificed 24 wpi. Muscle samples of 70g were stored at -21°C for 19, 30 and 56h, and for 1-8 weeks. Larvae were recovered by artificial digestion. Their mobilities were recorded using Saisam® image analysis software and their infectivities were evaluated using mouse bioassays. Samples frozen for 19, 30 and 56h allowed recovery of mobile ML, but samples frozen for 1 or 2 weeks did not. Correspondingly, only T. spiralis and T. britovi larvae isolated from wild boar meat frozen for 19, 30 and 56h established in mice. This study showed that freezing at -21°C for 1 week inactivated T. spiralis and T. britovi ML encapsulated in wild boar meat for24 weeks.
AB - Freeze-tolerance of encapsulated Trichinella muscle larvae (ML) is mainly determined by Trichinella species, but is also influenced by host species, the age of the infection and the storage time and temperature of the infected meat. Moreover, the freeze-tolerance of the encapsulated species appears to be correlated to the development of thick capsule walls which increases with age. An extended infection period and the muscle composition in some hosts (e.g. herbivores) may provide freeze-avoiding matrices due to high carbohydrate contents. The present experiment compares freeze-tolerance of Trichinella spiralis and Trichinella britovi ML in wild boar meat 24 weeks post inoculation (wpi). Three groups of four wild boars were infected with 200, 2000 or 20,000 ML of T. britovi (ISS 1575), respectively. Additionally, three wild boars were inoculated with 20,000 ML of T. spiralis (ISS 004) and two animals served as negative controls. All wild boars were sacrificed 24 wpi. Muscle samples of 70g were stored at -21°C for 19, 30 and 56h, and for 1-8 weeks. Larvae were recovered by artificial digestion. Their mobilities were recorded using Saisam® image analysis software and their infectivities were evaluated using mouse bioassays. Samples frozen for 19, 30 and 56h allowed recovery of mobile ML, but samples frozen for 1 or 2 weeks did not. Correspondingly, only T. spiralis and T. britovi larvae isolated from wild boar meat frozen for 19, 30 and 56h established in mice. This study showed that freezing at -21°C for 1 week inactivated T. spiralis and T. britovi ML encapsulated in wild boar meat for24 weeks.
KW - Trichinella spiralis
KW - Trichinella britovi
KW - Freezing
KW - Wild boars
KW - Sus scrofa
U2 - 10.1016/j.vetpar.2013.01.049
DO - 10.1016/j.vetpar.2013.01.049
M3 - Journal article
C2 - 23465440
SN - 0304-4017
VL - 194
SP - 175
EP - 178
JO - Veterinary Parasitology
JF - Veterinary Parasitology
IS - 2-4
ER -