Abstract
CONTEXT: Ferula foetida Regel (Apiaceae) is an Iranian medicinal plant with various biological activities including antispasmodic and anthelmintic.
OBJECTIVE: The sulfur compounds from the roots of F. foetida were isolated and characterized to test their cytotoxic and antimicrobial activities.
MATERIALS AND METHODS: The methanolic extract of the roots of F. foetida was fractionated using silica column chromatography. The components of each fraction were further purified using RP-HPLC. Their structures were elucidated by 1- and 2-D NMR spectroscopy as well as HREIMS. Their cytotoxic and antimicrobial activities were evaluated using Alamar Blue assay and broth microdilution method, respectively.
RESULTS: Four new thiophene derivatives, namely foetithiophenes C-F (3-6), together with four known compounds, foetithiophenes A (1) and B (2), coniferaldehyde, and sinapic aldehyde, were isolated from the roots of F. foetida. Antimicrobial activities were observed in particular against the Gram-positive bacteria. The best antimicrobial activity was observed for compound 6 against B. cereus with a MIC value 50 µg/mL. The tested compounds did not show cytotoxic properties against MCF-7 and K562 cells.
CONCLUSION: Four new thiophene derivatives including foetithiophenes C-F (3-6) were characterized from the roots of F. foetida. Foetithiophene F (6) exhibited the most potent activity against the Gram-positive bacteria B. cereus.
| Original language | English |
|---|---|
| Journal | Pharmaceutical Biology |
| Volume | 53 |
| Issue number | 5 |
| Pages (from-to) | 710-4 |
| Number of pages | 5 |
| ISSN | 1388-0209 |
| DOIs | |
| Publication status | Published - 1 May 2015 |
Keywords
- Anti-Infective Agents/isolation & purification
- Ferula
- Humans
- K562 Cells
- MCF-7 Cells
- Microbial Sensitivity Tests/methods
- Plant Extracts/isolation & purification
- Plant Roots
- Thiophenes/isolation & purification