Fluorescent biosensor for the detection of hyaluronidase: intensity-based ratiometric sensing and fluorescence lifetime-based sensing using a long lifetime azadioxatriangulenium (ADOTA) fluorophore

Rahul Chib; Mark Mummert; Ilkay Bora; Bo Wegge Laursen; Sunil Shah; Robert Pendry; Ignacy Gryczynski; Julian Borejdo; Zygmunt Gryczynski; Rafal Fudala

doi:10.1007/s00216-016-9472-5

Fluorescent biosensor for the detection of hyaluronidase: intensity-based ratiometric sensing and fluorescence lifetime-based sensing using a long lifetime azadioxatriangulenium (ADOTA) fluorophore

Rahul Chib, Mark Mummert, Ilkay Bora, Bo Wegge Laursen, Sunil Shah, Robert Pendry, Ignacy Gryczynski, Julian Borejdo, Zygmunt Gryczynski, Rafal Fudala

Department of Chemistry

8 Citations (Scopus)

Original language	English
Journal	Analytical and Bioanalytical Chemistry
Volume	408
Issue number	14
Pages (from-to)	3811-3821
Number of pages	11
ISSN	1618-2642
DOIs	https://doi.org/10.1007/s00216-016-9472-5
Publication status	Published - 1 May 2016

Keywords

Hyaluronidase sensing
Azadioxatriangulenium fluorophore
Ratiometric sensing
Fluorescence lifetime-based sensing
Fluorescence-based assay

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10.1007/s00216-016-9472-5

Cite this

Chib, R., Mummert, M., Bora, I., Laursen, B. W., Shah, S., Pendry, R., Gryczynski, I., Borejdo, J., Gryczynski, Z., & Fudala, R. (2016). Fluorescent biosensor for the detection of hyaluronidase: intensity-based ratiometric sensing and fluorescence lifetime-based sensing using a long lifetime azadioxatriangulenium (ADOTA) fluorophore. Analytical and Bioanalytical Chemistry, 408(14), 3811-3821. https://doi.org/10.1007/s00216-016-9472-5

Fluorescent biosensor for the detection of hyaluronidase: intensity-based ratiometric sensing and fluorescence lifetime-based sensing using a long lifetime azadioxatriangulenium (ADOTA) fluorophore. / Chib, Rahul; Mummert, Mark; Bora, Ilkay et al.
In: Analytical and Bioanalytical Chemistry, Vol. 408, No. 14, 01.05.2016, p. 3811-3821.

Research output: Contribution to journal › Journal article › Research › peer-review

Chib, R, Mummert, M, Bora, I, Laursen, BW, Shah, S, Pendry, R, Gryczynski, I, Borejdo, J, Gryczynski, Z & Fudala, R 2016, 'Fluorescent biosensor for the detection of hyaluronidase: intensity-based ratiometric sensing and fluorescence lifetime-based sensing using a long lifetime azadioxatriangulenium (ADOTA) fluorophore', Analytical and Bioanalytical Chemistry, vol. 408, no. 14, pp. 3811-3821. https://doi.org/10.1007/s00216-016-9472-5

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keywords = "Hyaluronidase sensing, Azadioxatriangulenium fluorophore, Ratiometric sensing, Fluorescence lifetime-based sensing, Fluorescence-based assay",

author = "Rahul Chib and Mark Mummert and Ilkay Bora and Laursen, {Bo Wegge} and Sunil Shah and Robert Pendry and Ignacy Gryczynski and Julian Borejdo and Zygmunt Gryczynski and Rafal Fudala",

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AU - Chib, Rahul

AU - Mummert, Mark

AU - Bora, Ilkay

AU - Laursen, Bo Wegge

AU - Shah, Sunil

AU - Pendry, Robert

AU - Gryczynski, Ignacy

AU - Borejdo, Julian

AU - Gryczynski, Zygmunt

AU - Fudala, Rafal

PY - 2016/5/1

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KW - Hyaluronidase sensing

KW - Azadioxatriangulenium fluorophore

KW - Ratiometric sensing

KW - Fluorescence lifetime-based sensing

KW - Fluorescence-based assay

U2 - 10.1007/s00216-016-9472-5

DO - 10.1007/s00216-016-9472-5

M3 - Journal article

C2 - 26993308

SN - 1618-2642

VL - 408

SP - 3811

EP - 3821

JO - Analytical and Bioanalytical Chemistry

JF - Analytical and Bioanalytical Chemistry

IS - 14

ER -

Fluorescent biosensor for the detection of hyaluronidase: intensity-based ratiometric sensing and fluorescence lifetime-based sensing using a long lifetime azadioxatriangulenium (ADOTA) fluorophore

Keywords

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