Fluorescence in situ hybridization for the tissue detection of bacterial pathogens associated with porcine infections.

Henrik Elvang Jensen; Louise Kruse Jensen; Kristiane Barington; Susanne Elisabeth Pors; Thomas Bjarnsholt; Mette Boye

doi:10.1007/978-1-4939-2004-4_17

Fluorescence in situ hybridization for the tissue detection of bacterial pathogens associated with porcine infections.

Henrik Elvang Jensen, Louise Kruse Jensen, Kristiane Barington, Susanne Elisabeth Pors, Thomas Bjarnsholt, Mette Boye

2 Citations (Scopus)

Abstract

Fluorescence in situ hybridization (FISH) is an efficient technique for the identification of specific bacteria in tissue of both experimental and spontaneous infections. The method detects specific sequences of nucleic acids by hybridization of fluorescently labeled probes to complementary target sequences within intact cells. FISH allows direct histological localization of the bacteria in the tissue and thereby a correlation between the infection and the histopathological changes present. This chapter presents protocols for FISH identification of bacterial pathogens in fixed deparaffinized tissue samples mounted on glass slides. Two different methods are presented: one is illustrated with the use of peptide nucleic acid (PNA) that is carried out directly on glass slides (Method I), whereas the other is exemplified by using a DNA probe in a Shandon rack (Method II). In the two methods, both PNA and DNA probes can be used.

Original language	English
Journal	Methods in Molecular Biology
Volume	1247
Pages (from-to)	219-134
Number of pages	16
ISSN	1064-3745
DOIs	https://doi.org/10.1007/978-1-4939-2004-4_17
Publication status	Published - 15 Nov 2014

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title = "Fluorescence in situ hybridization for the tissue detection of bacterial pathogens associated with porcine infections.",

abstract = "Fluorescence in situ hybridization (FISH) is an efficient technique for the identification of specific bacteria in tissue of both experimental and spontaneous infections. The method detects specific sequences of nucleic acids by hybridization of fluorescently labeled probes to complementary target sequences within intact cells. FISH allows direct histological localization of the bacteria in the tissue and thereby a correlation between the infection and the histopathological changes present. This chapter presents protocols for FISH identification of bacterial pathogens in fixed deparaffinized tissue samples mounted on glass slides. Two different methods are presented: one is illustrated with the use of peptide nucleic acid (PNA) that is carried out directly on glass slides (Method I), whereas the other is exemplified by using a DNA probe in a Shandon rack (Method II). In the two methods, both PNA and DNA probes can be used.",

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T1 - Fluorescence in situ hybridization for the tissue detection of bacterial pathogens associated with porcine infections.

AU - Jensen, Henrik Elvang

AU - Jensen, Louise Kruse

AU - Barington, Kristiane

AU - Pors, Susanne Elisabeth

AU - Bjarnsholt, Thomas

AU - Boye, Mette

PY - 2014/11/15

Y1 - 2014/11/15

N2 - Fluorescence in situ hybridization (FISH) is an efficient technique for the identification of specific bacteria in tissue of both experimental and spontaneous infections. The method detects specific sequences of nucleic acids by hybridization of fluorescently labeled probes to complementary target sequences within intact cells. FISH allows direct histological localization of the bacteria in the tissue and thereby a correlation between the infection and the histopathological changes present. This chapter presents protocols for FISH identification of bacterial pathogens in fixed deparaffinized tissue samples mounted on glass slides. Two different methods are presented: one is illustrated with the use of peptide nucleic acid (PNA) that is carried out directly on glass slides (Method I), whereas the other is exemplified by using a DNA probe in a Shandon rack (Method II). In the two methods, both PNA and DNA probes can be used.

AB - Fluorescence in situ hybridization (FISH) is an efficient technique for the identification of specific bacteria in tissue of both experimental and spontaneous infections. The method detects specific sequences of nucleic acids by hybridization of fluorescently labeled probes to complementary target sequences within intact cells. FISH allows direct histological localization of the bacteria in the tissue and thereby a correlation between the infection and the histopathological changes present. This chapter presents protocols for FISH identification of bacterial pathogens in fixed deparaffinized tissue samples mounted on glass slides. Two different methods are presented: one is illustrated with the use of peptide nucleic acid (PNA) that is carried out directly on glass slides (Method I), whereas the other is exemplified by using a DNA probe in a Shandon rack (Method II). In the two methods, both PNA and DNA probes can be used.

U2 - 10.1007/978-1-4939-2004-4_17

DO - 10.1007/978-1-4939-2004-4_17

M3 - Journal article

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SN - 1064-3745

VL - 1247

SP - 219

EP - 134

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Fluorescence in situ hybridization for the tissue detection of bacterial pathogens associated with porcine infections.

Abstract

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