Flavobacterium sp. strain 4221 and Pedobacter sp. strain 4236 beta-1,3-glucanases that are active at low temperatures

Mikkel Aabech Rasmussen; Søren Madsen; Peter Stougaard; Mads Grønvald Johnsen

doi:10.1128/AEM.00681-08

Flavobacterium sp. strain 4221 and Pedobacter sp. strain 4236 beta-1,3-glucanases that are active at low temperatures

Mikkel Aabech Rasmussen, Søren Madsen, Peter Stougaard, Mads Grønvald Johnsen

12 Citations (Scopus)

Abstract

Secretion of beta-1,3-glucanases by the arctic bacterial isolates 4221 and 4236, related to the genera Flavobacterium and Pedobacter, was discovered. Escherichia coli and Lactococcus lactis expression of beta-1,3-glucanases Glc4221-1 and Glc4236-1 from the respective isolates was achieved. The enzymes hydrolyzed fungal cell walls and retained activity at low temperatures.

Original language	English
Journal	Applied and Environmental Microbiology
Volume	74
Issue number	22
Pages (from-to)	7070-7072
Number of pages	3
ISSN	0099-2240
DOIs	https://doi.org/10.1128/AEM.00681-08
Publication status	Published - 2008
Externally published	Yes

Keywords

Amino Acid Sequence
Bacteroidetes
Base Sequence
Cell Wall
Cloning, Molecular
Cold Temperature
Escherichia coli
Flavobacterium
Gene Expression
Glucan 1,3-beta-Glucosidase
Lactococcus lactis
Molecular Sequence Data
Temperature

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10.1128/AEM.00681-08

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@article{e77ec6ba484a4d7f865897bd45606778,

title = "Flavobacterium sp. strain 4221 and Pedobacter sp. strain 4236 beta-1,3-glucanases that are active at low temperatures",

abstract = "Secretion of beta-1,3-glucanases by the arctic bacterial isolates 4221 and 4236, related to the genera Flavobacterium and Pedobacter, was discovered. Escherichia coli and Lactococcus lactis expression of beta-1,3-glucanases Glc4221-1 and Glc4236-1 from the respective isolates was achieved. The enzymes hydrolyzed fungal cell walls and retained activity at low temperatures.",

keywords = "Amino Acid Sequence, Bacteroidetes, Base Sequence, Cell Wall, Cloning, Molecular, Cold Temperature, Escherichia coli, Flavobacterium, Gene Expression, Glucan 1,3-beta-Glucosidase, Lactococcus lactis, Molecular Sequence Data, Temperature",

author = "Rasmussen, {Mikkel Aabech} and S{\o}ren Madsen and Peter Stougaard and Johnsen, {Mads Gr{\o}nvald}",

year = "2008",

doi = "10.1128/AEM.00681-08",

language = "English",

volume = "74",

pages = "7070--7072",

journal = "Applied and Environmental Microbiology",

issn = "0099-2240",

publisher = "American Society for Microbiology",

number = "22",

}

TY - JOUR

T1 - Flavobacterium sp. strain 4221 and Pedobacter sp. strain 4236 beta-1,3-glucanases that are active at low temperatures

AU - Rasmussen, Mikkel Aabech

AU - Madsen, Søren

AU - Stougaard, Peter

AU - Johnsen, Mads Grønvald

PY - 2008

Y1 - 2008

N2 - Secretion of beta-1,3-glucanases by the arctic bacterial isolates 4221 and 4236, related to the genera Flavobacterium and Pedobacter, was discovered. Escherichia coli and Lactococcus lactis expression of beta-1,3-glucanases Glc4221-1 and Glc4236-1 from the respective isolates was achieved. The enzymes hydrolyzed fungal cell walls and retained activity at low temperatures.

AB - Secretion of beta-1,3-glucanases by the arctic bacterial isolates 4221 and 4236, related to the genera Flavobacterium and Pedobacter, was discovered. Escherichia coli and Lactococcus lactis expression of beta-1,3-glucanases Glc4221-1 and Glc4236-1 from the respective isolates was achieved. The enzymes hydrolyzed fungal cell walls and retained activity at low temperatures.

KW - Amino Acid Sequence

KW - Bacteroidetes

KW - Base Sequence

KW - Cell Wall

KW - Cloning, Molecular

KW - Cold Temperature

KW - Escherichia coli

KW - Flavobacterium

KW - Gene Expression

KW - Glucan 1,3-beta-Glucosidase

KW - Lactococcus lactis

KW - Molecular Sequence Data

KW - Temperature

U2 - 10.1128/AEM.00681-08

DO - 10.1128/AEM.00681-08

M3 - Journal article

C2 - 18806001

SN - 0099-2240

VL - 74

SP - 7070

EP - 7072

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 22

ER -

Flavobacterium sp. strain 4221 and Pedobacter sp. strain 4236 beta-1,3-glucanases that are active at low temperatures

Abstract

Keywords

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