Fetal effects of epidermal growth factor deficiency induced in rats by autoantibodies against epidermal growth factor

TY - JOUR

T1 - Fetal effects of epidermal growth factor deficiency induced in rats by autoantibodies against epidermal growth factor

AU - Raaberg, Lasse

AU - Nexø, Ebba

AU - Jørgensen, P E

AU - Poulsen, Steen Seier

AU - Jakab, M

PY - 1995/2

Y1 - 1995/2

N2 - We have used rats with epidermal growth factor (EGF) autoantibodies to study the role of EGF deficiency during perinatal development. The study was focused on organs known to contain EGF or its receptor. Compared with controls, the offspring of autoimmune rats had a higher perinatal mortality and a lower birth weight. The weight of the lungs was particularly low in the offspring of EGF-immunized rats, and morphologically the lungs from the surviving pups seemed atelectatic and had alveolar duct dilatation, which indicates mild respiratory distress syndrome. Judged from immunohistochemical studies, the amount of surfactant protein-A was decreased, suggesting a delayed lung maturation. The offspring of EGF-immunized rats had dry and wrinkled skin. The skin was thin and the hair follicles were immature. This suggests a role for EGF in the growth and development of the skin. The liver/body weight ratio was lower in pups from EGF-immunized rats. This difference was, however, not significant (p = 0.07), but flow cytometric analyses showed a significantly lower proportion of the liver cells from newborn EGF-deficient pups to be in S-phase and indicated that these cells were larger than liver cells from controls. To study possible alterations in EGF binding, 125I-EGF was injected i.v. in newborn rats. 125I-EGF bound in all the organs investigated. The binding is listed in decreasing order: liver, gut, skin, kidney, and lungs. In the pups from EGF-immunized rats, the lungs and the skin bound a significantly higher amount than the controls.(ABSTRACT TRUNCATED AT 250 WORDS)

AB - We have used rats with epidermal growth factor (EGF) autoantibodies to study the role of EGF deficiency during perinatal development. The study was focused on organs known to contain EGF or its receptor. Compared with controls, the offspring of autoimmune rats had a higher perinatal mortality and a lower birth weight. The weight of the lungs was particularly low in the offspring of EGF-immunized rats, and morphologically the lungs from the surviving pups seemed atelectatic and had alveolar duct dilatation, which indicates mild respiratory distress syndrome. Judged from immunohistochemical studies, the amount of surfactant protein-A was decreased, suggesting a delayed lung maturation. The offspring of EGF-immunized rats had dry and wrinkled skin. The skin was thin and the hair follicles were immature. This suggests a role for EGF in the growth and development of the skin. The liver/body weight ratio was lower in pups from EGF-immunized rats. This difference was, however, not significant (p = 0.07), but flow cytometric analyses showed a significantly lower proportion of the liver cells from newborn EGF-deficient pups to be in S-phase and indicated that these cells were larger than liver cells from controls. To study possible alterations in EGF binding, 125I-EGF was injected i.v. in newborn rats. 125I-EGF bound in all the organs investigated. The binding is listed in decreasing order: liver, gut, skin, kidney, and lungs. In the pups from EGF-immunized rats, the lungs and the skin bound a significantly higher amount than the controls.(ABSTRACT TRUNCATED AT 250 WORDS)

KW - Abnormalities, Multiple

KW - Animals

KW - Autoantibodies

KW - Birth Weight

KW - Digestive System

KW - Embryonic and Fetal Development

KW - Epidermal Growth Factor

KW - Female

KW - Immunization

KW - Liver

KW - Lung

KW - Pregnancy

KW - Pregnancy Complications

KW - Pulmonary Atelectasis

KW - Rats

KW - Rats, Wistar

KW - Skin

U2 - 10.1203/00006450-199502000-00009

DO - 10.1203/00006450-199502000-00009

M3 - Journal article

C2 - 7731754

SN - 0031-3998

VL - 37

SP - 175

EP - 181

JO - Pediatric Research

JF - Pediatric Research

IS - 2

ER -