Abstract
The VAMPs/synaptobrevins (Vp/Sybs) are small integral membrane proteins. Two isoforms, Vp/Syb 1 and Vp/Syb 2, are considered to be specific to neural tissue. They are associated with synaptic vesicles and are believed to play an important role in neurotransmitter release. A third isoform, cellubrevin, has recently been found in non-neural tissues. We now report that the distribution of Vp/Syb 1 and Vp/Syb 2 is wider than previously thought. RNA transcripts for both Vp/Syb 1 and Vp/Syb 2 were found in rat skeletal muscle and in several other rat non-neural tissues, and antibodies specific for Vp/Syb 2 detected a protein in the endoplasmic reticulum-Golgi area of skeletal muscle. Thus Vp/Sybs 1 and 2 are not restricted to the nervous system but appear to be co-expressed with cellubrevin in many different tissues. This redundancy of Vp/Sybs in a single cell may be required to control the specificity of vesicle-target interaction in the several pathways of intracellular vesicle traffic that are operative within each cell.
| Original language | English |
|---|---|
| Journal | The Journal of Biological Chemistry |
| Volume | 269 |
| Issue number | 22 |
| Pages (from-to) | 15403-6 |
| Number of pages | 4 |
| ISSN | 0021-9258 |
| Publication status | Published - 3 Jun 1994 |
Keywords
- Adipose Tissue, Brown
- Amino Acid Sequence
- Animals
- Brain
- Endoplasmic Reticulum
- Fluorescent Antibody Technique
- Gene Expression
- Golgi Apparatus
- Male
- Membrane Proteins
- Molecular Sequence Data
- Muscles
- Nerve Tissue Proteins
- Polymerase Chain Reaction
- R-SNARE Proteins
- Rats
- Rats, Wistar
- Sequence Homology, Amino Acid
- Transcription, Genetic