Expression of the Arabidopsis high-affinity hexose transporter STP13 correlates with programmed cell death.

TY - JOUR

T1 - Expression of the Arabidopsis high-affinity hexose transporter STP13 correlates with programmed cell death.

AU - Nørholm, Morten Helge Hauberg

AU - Nour-Eldin, Hussam H

AU - Brodersen, Peter

AU - Mundy, John

AU - Halkjær, Barbara Ann

N1 - Keywords: Animals; Apoptosis; Apoptosis Regulatory Proteins; Arabidopsis; Arabidopsis Proteins; Cell Aging; Cells, Cultured; Enzyme Inhibitors; Fumonisins; Gene Expression; Membrane Transport Proteins; Monosaccharide Transport Proteins; Oocytes; Symporters; Xenopus

PY - 2006

Y1 - 2006

N2 - We report the biochemical characterization in Xenopus oocytes of the Arabidopsis thaliana membrane protein, STP13, as a high affinity, hexose-specific H(+)-symporter. Studies with kinase activators suggest that it is negatively regulated by phosphorylation. STP13 promoter GFP reporter lines show GFP expression only in the vascular tissue in emerging petals under non-stressed conditions. Quantitative PCR and the pSTP13-GFP plants show induction of STP13 in programmed cell death (PCD) obtained by treatments with the fungal toxin fumonisin B1 and the pathogen Pseudomonas syringae. A role for STP13 in PCD is supported by microarray data from e.g. plants undergoing senescence and a strong correlation between STP13 transcripts and the PCD phenotype in different accelerated cell death (acd11) mutants.

AB - We report the biochemical characterization in Xenopus oocytes of the Arabidopsis thaliana membrane protein, STP13, as a high affinity, hexose-specific H(+)-symporter. Studies with kinase activators suggest that it is negatively regulated by phosphorylation. STP13 promoter GFP reporter lines show GFP expression only in the vascular tissue in emerging petals under non-stressed conditions. Quantitative PCR and the pSTP13-GFP plants show induction of STP13 in programmed cell death (PCD) obtained by treatments with the fungal toxin fumonisin B1 and the pathogen Pseudomonas syringae. A role for STP13 in PCD is supported by microarray data from e.g. plants undergoing senescence and a strong correlation between STP13 transcripts and the PCD phenotype in different accelerated cell death (acd11) mutants.

U2 - 10.1016/j.febslet.2006.03.064

DO - 10.1016/j.febslet.2006.03.064

M3 - Journal article

C2 - 16616142

SN - 0014-5793

VL - 580

SP - 2381

EP - 2387

JO - FEBS Letters

JF - FEBS Letters

IS - 9

ER -