TY - JOUR
T1 - Expression of recombinant proteins: an introduction
AU - Nøhr, Jane
AU - Kristiansen, Karsten
AU - Krogsdam, Anne-M
N1 - Keywords: Animals; Biotechnology; Protein Engineering; Recombinant Proteins
PY - 2003
Y1 - 2003
N2 - To better understand and characterize the function (or lack thereof) of proteins-wild-type vs mutants-it is advantageous to be able to express these proteins in a controlled, chosen setup. For downstream applications such as enzymatic assays, X-ray crystallography/nuclear magnetic resonance (NMR), raising antibodies, or characterization of physical interaction with other proteins, it may furthermore be desirable to obtain large quantities of pure protein.
AB - To better understand and characterize the function (or lack thereof) of proteins-wild-type vs mutants-it is advantageous to be able to express these proteins in a controlled, chosen setup. For downstream applications such as enzymatic assays, X-ray crystallography/nuclear magnetic resonance (NMR), raising antibodies, or characterization of physical interaction with other proteins, it may furthermore be desirable to obtain large quantities of pure protein.
U2 - 10.1385/1-59259-394-1:93
DO - 10.1385/1-59259-394-1:93
M3 - Journal article
C2 - 12840542
SN - 1064-3745
VL - 232
SP - 93
EP - 101
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
ER -