Expression of IGF-II mRNA-binding proteins (IMPs) in gonads and testicular cancer

TY - JOUR

T1 - Expression of IGF-II mRNA-binding proteins (IMPs) in gonads and testicular cancer

AU - Hammer, Niels A

AU - Hansen, Thomas v O

AU - Byskov, Anne Grete

AU - Rajpert-De Meyts, Eva

AU - Grøndahl, Marie Louise

AU - Bredkjaer, Helle E

AU - Wewer, Ulla M

AU - Christiansen, Jan

AU - Nielsen, Finn C

PY - 2005

Y1 - 2005

N2 - Insulin-like growth factor-II mRNA-binding proteins 1, 2 and 3 (IMP1, IMP2 and IMP3) belong to a family of RNA-binding proteins implicated in mRNA localization, turnover and translational control. We examined their expression pattern during development of murine and human testis and ovaries. In the mouse, IMPs were expressed in male and female gonadal cells at embryonic day 12.5 (E12.5). From E16.5, IMP1 and IMP3 became restricted to the developing germ cells, whereas IMP2 expression persisted in the interstitial cells. In mature mouse and human ovaries, IMP1, IMP2 and IMP3 were detected in resting and growing oocytes and in the granulosa cells. In testis, IMP1 and IMP3 were found mainly in the spermatogonia, whereas IMP2 was expressed in the immature Leydig cells. Moreover, all three IMPs were detected in human semen. The developmental expression pattern of IMP1 and IMP3 in the human testis prompted us to examine their possible involvement in testicular neoplasia. IMPs were detected primarily in germ-cell neoplasms, including preinvasive testicular carcinoma in situ, classical and spermatocytic seminoma, and nonseminomas, with particularly high expression in undifferentiated embryonal carcinoma. The relative expression of IMP1, IMP2 and IMP3 varied among tumor types and only IMP1 was detected in all carcinoma in situ cells. Thus IMPs, and in particular IMP1, may be useful auxiliary markers of testicular neoplasia.

AB - Insulin-like growth factor-II mRNA-binding proteins 1, 2 and 3 (IMP1, IMP2 and IMP3) belong to a family of RNA-binding proteins implicated in mRNA localization, turnover and translational control. We examined their expression pattern during development of murine and human testis and ovaries. In the mouse, IMPs were expressed in male and female gonadal cells at embryonic day 12.5 (E12.5). From E16.5, IMP1 and IMP3 became restricted to the developing germ cells, whereas IMP2 expression persisted in the interstitial cells. In mature mouse and human ovaries, IMP1, IMP2 and IMP3 were detected in resting and growing oocytes and in the granulosa cells. In testis, IMP1 and IMP3 were found mainly in the spermatogonia, whereas IMP2 was expressed in the immature Leydig cells. Moreover, all three IMPs were detected in human semen. The developmental expression pattern of IMP1 and IMP3 in the human testis prompted us to examine their possible involvement in testicular neoplasia. IMPs were detected primarily in germ-cell neoplasms, including preinvasive testicular carcinoma in situ, classical and spermatocytic seminoma, and nonseminomas, with particularly high expression in undifferentiated embryonal carcinoma. The relative expression of IMP1, IMP2 and IMP3 varied among tumor types and only IMP1 was detected in all carcinoma in situ cells. Thus IMPs, and in particular IMP1, may be useful auxiliary markers of testicular neoplasia.

KW - Animals

KW - Blastocyst

KW - Blotting, Western

KW - Carcinoma in Situ

KW - Carcinoma, Embryonal

KW - Embryonic Development

KW - Female

KW - Gestational Age

KW - Gonads

KW - Granulosa Cells

KW - Humans

KW - Immunohistochemistry

KW - Insulin-Like Growth Factor II

KW - Leydig Cells

KW - Male

KW - Mice

KW - Neoplasm Proteins

KW - Neoplasms, Germ Cell and Embryonal

KW - Oocytes

KW - RNA-Binding Proteins

KW - Semen

KW - Seminoma

KW - Spermatozoa

KW - Testicular Neoplasms

KW - Testis

KW - Tumor Markers, Biological

U2 - 10.1530/rep.1.00664

DO - 10.1530/rep.1.00664

M3 - Journal article

C2 - 16049158

SN - 1470-1626

VL - 130

SP - 203

EP - 212

JO - Reproduction

JF - Reproduction

IS - 2

ER -