Expression of a type B RIFIN in Plasmodium falciparum merozoites and gametes

TY - JOUR

T1 - Expression of a type B RIFIN in Plasmodium falciparum merozoites and gametes

AU - Mwakalinga, Steven B

AU - Wang, Christian W

AU - Bengtsson, Dominique C

AU - Turner, Louise

AU - Dinko, Bismarck

AU - Lusingu, John P

AU - Arnot, David E

AU - Sutherland, Colin J

AU - Theander, Thor G

AU - Lavstsen, Thomas

PY - 2012

Y1 - 2012

N2 - Background: The ability of Plasmodium falciparum to undergo antigenic variation, by switching expression among protein variants encoded by multigene families, such as var, rif and stevor, is key to the survival of this parasite in the human host. The RIFIN protein family can be divided into A and B types based on the presence or absence of a 25 amino acid motif in the semi-conserved domain. A particular type B RIFIN, PF13-0006, has previously been shown to be strongly transcribed in the asexual and sexual stages of P. falciparum in vitro. Methods. Antibodies to recombinant PF13-0006 RIFIN were used in immunofluorescence and confocal imaging of 3D7 parasites throughout the asexual reproduction and sexual development to examine the expression of PF13-0006. Furthermore, reactivity to recombinant PF13-0006 was measured in plasma samples collected from individuals from both East and West African endemic areas. Results: The PF13-0006 RIFIN variant appeared expressed by both released merozoites and gametes after emergence. 7.4% and 12.1% of individuals from East and West African endemic areas, respectively, carry plasma antibodies that recognize recombinant PF13-0006, where the antibody responses were more common among older children. Conclusions: The stage specificity of PF13-0006 suggests that the diversity of RIFIN variants has evolved to provide multiple specialized functions in different stages of the parasite life cycle. These data also suggest that RIFIN variants antigenically similar to PF13-0006 occur in African parasite populations.

AB - Background: The ability of Plasmodium falciparum to undergo antigenic variation, by switching expression among protein variants encoded by multigene families, such as var, rif and stevor, is key to the survival of this parasite in the human host. The RIFIN protein family can be divided into A and B types based on the presence or absence of a 25 amino acid motif in the semi-conserved domain. A particular type B RIFIN, PF13-0006, has previously been shown to be strongly transcribed in the asexual and sexual stages of P. falciparum in vitro. Methods. Antibodies to recombinant PF13-0006 RIFIN were used in immunofluorescence and confocal imaging of 3D7 parasites throughout the asexual reproduction and sexual development to examine the expression of PF13-0006. Furthermore, reactivity to recombinant PF13-0006 was measured in plasma samples collected from individuals from both East and West African endemic areas. Results: The PF13-0006 RIFIN variant appeared expressed by both released merozoites and gametes after emergence. 7.4% and 12.1% of individuals from East and West African endemic areas, respectively, carry plasma antibodies that recognize recombinant PF13-0006, where the antibody responses were more common among older children. Conclusions: The stage specificity of PF13-0006 suggests that the diversity of RIFIN variants has evolved to provide multiple specialized functions in different stages of the parasite life cycle. These data also suggest that RIFIN variants antigenically similar to PF13-0006 occur in African parasite populations.

U2 - 10.1186/1475-2875-11-429

DO - 10.1186/1475-2875-11-429

M3 - Journal article

C2 - 23259643

SN - 1475-2875

VL - 11

SP - 429

JO - Malaria Journal

JF - Malaria Journal

IS - 1

ER -