Exploiting Femtoliter Microwells for the Sensitive Measurement of Protein Adsorption

TY - JOUR

T1 - Exploiting Femtoliter Microwells for the Sensitive Measurement of Protein Adsorption

AU - Nishioka, Gary M

AU - Geczy, Reka

AU - Huggler, Kimberly S

AU - Dao, Tram N

AU - Sokolik, Charles W

PY - 2017/7/18

Y1 - 2017/7/18

N2 - A method is described for the sensitive measurement of adsorbed proteins using femtoliter microwells. Quantitative measurement of adsorbed protein is demonstrated at surface densities from 10 fg/cm(2) to 3 pg/cm(2). Determination of the efficacy of barrier coatings is also demonstrated using femtoliter microwells. Adsorption at low surface densities is measured, indicating the highest affinity sites on the surface and therefore the initial stages of adsorption. The femtoliter microwell method is shown to be useful in detecting differences between effective protective coatings.

AB - A method is described for the sensitive measurement of adsorbed proteins using femtoliter microwells. Quantitative measurement of adsorbed protein is demonstrated at surface densities from 10 fg/cm(2) to 3 pg/cm(2). Determination of the efficacy of barrier coatings is also demonstrated using femtoliter microwells. Adsorption at low surface densities is measured, indicating the highest affinity sites on the surface and therefore the initial stages of adsorption. The femtoliter microwell method is shown to be useful in detecting differences between effective protective coatings.

KW - Journal Article

U2 - 10.1021/acs.langmuir.7b00819

DO - 10.1021/acs.langmuir.7b00819

M3 - Journal article

C2 - 28666390

SN - 0743-7463

VL - 33

SP - 6985

EP - 6990

JO - Langmuir : the ACS journal of surfaces and colloids

JF - Langmuir : the ACS journal of surfaces and colloids

IS - 28

ER -