Experimental identification of microRNA targets

Ulf Andersson Ørom; Anders H Lund

doi:10.1016/j.gene.2009.11.008

Experimental identification of microRNA targets

Ulf Andersson Ørom, Anders H Lund

72 Citations (Scopus)

Abstract

microRNAs are small RNAs that regulate protein synthesis post-transcriptionally. Animal microRNAs recognize their targets by incomplete base pairing to sequence motifs most often present in the 3' untranslated region of their target mRNAs. This partial complementarity vastly expands the repertoire of potential targets and constitutes a problem for computational target prediction. Although computational analyses have shed light on important aspects of microRNA target recognition, several questions remain regarding how microRNAs can recognize and regulate their targets. Forward experimental approaches allow for an unbiased study of microRNA target recognition and may unveil novel, rare or uncommon target binding patterns. In this review we focus on animal microRNAs and the experimental approaches that have been described for identification of their targets.

Original language	English
Journal	Gene
Volume	451
Issue number	1-2
Pages (from-to)	1-5
Number of pages	4
ISSN	0378-1119
DOIs	https://doi.org/10.1016/j.gene.2009.11.008
Publication status	Published - 2009

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10.1016/j.gene.2009.11.008

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title = "Experimental identification of microRNA targets",

abstract = "microRNAs are small RNAs that regulate protein synthesis post-transcriptionally. Animal microRNAs recognize their targets by incomplete base pairing to sequence motifs most often present in the 3' untranslated region of their target mRNAs. This partial complementarity vastly expands the repertoire of potential targets and constitutes a problem for computational target prediction. Although computational analyses have shed light on important aspects of microRNA target recognition, several questions remain regarding how microRNAs can recognize and regulate their targets. Forward experimental approaches allow for an unbiased study of microRNA target recognition and may unveil novel, rare or uncommon target binding patterns. In this review we focus on animal microRNAs and the experimental approaches that have been described for identification of their targets.",

author = "{\O}rom, {Ulf Andersson} and Lund, {Anders H}",

note = "Keywords: 3' Untranslated Regions; Animals; Base Pairing; Gene Expression Regulation; Genetic Techniques; MicroRNAs; Proteins; RNA, Messenger",

year = "2009",

doi = "10.1016/j.gene.2009.11.008",

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T1 - Experimental identification of microRNA targets

AU - Ørom, Ulf Andersson

AU - Lund, Anders H

N1 - Keywords: 3' Untranslated Regions; Animals; Base Pairing; Gene Expression Regulation; Genetic Techniques; MicroRNAs; Proteins; RNA, Messenger

PY - 2009

Y1 - 2009

N2 - microRNAs are small RNAs that regulate protein synthesis post-transcriptionally. Animal microRNAs recognize their targets by incomplete base pairing to sequence motifs most often present in the 3' untranslated region of their target mRNAs. This partial complementarity vastly expands the repertoire of potential targets and constitutes a problem for computational target prediction. Although computational analyses have shed light on important aspects of microRNA target recognition, several questions remain regarding how microRNAs can recognize and regulate their targets. Forward experimental approaches allow for an unbiased study of microRNA target recognition and may unveil novel, rare or uncommon target binding patterns. In this review we focus on animal microRNAs and the experimental approaches that have been described for identification of their targets.

AB - microRNAs are small RNAs that regulate protein synthesis post-transcriptionally. Animal microRNAs recognize their targets by incomplete base pairing to sequence motifs most often present in the 3' untranslated region of their target mRNAs. This partial complementarity vastly expands the repertoire of potential targets and constitutes a problem for computational target prediction. Although computational analyses have shed light on important aspects of microRNA target recognition, several questions remain regarding how microRNAs can recognize and regulate their targets. Forward experimental approaches allow for an unbiased study of microRNA target recognition and may unveil novel, rare or uncommon target binding patterns. In this review we focus on animal microRNAs and the experimental approaches that have been described for identification of their targets.

U2 - 10.1016/j.gene.2009.11.008

DO - 10.1016/j.gene.2009.11.008

M3 - Journal article

C2 - 19944134

SN - 0378-1119

VL - 451

SP - 1

EP - 5

JO - Gene

JF - Gene

IS - 1-2

ER -

Experimental identification of microRNA targets

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