EXPANSIN A1-mediated radial swelling of pericycle cells positions anticlinal cell divisions during lateral root initiation

Priya Ramakrishna; Paola Ruiz Duarte; Graham A. Rance; Martin Schubert; Vera Vordermaier; Lam Dai Vu; Evan Murphy; Amaya Vilches Barro; Kamal Swarup; Kamaljit Moirangthem; Bodil Jørgensen; Brigitte Van De Cotte; Tatsuaki Goh; Zhefeng Lin; Ute Voβ; Tom Beeckman; Malcolm J. Bennett; Kris Gevaert; Alexis Maizel; Ive De Smet

doi:10.1073/pnas.1820882116

EXPANSIN A1-mediated radial swelling of pericycle cells positions anticlinal cell divisions during lateral root initiation

Priya Ramakrishna, Paola Ruiz Duarte, Graham A. Rance, Martin Schubert, Vera Vordermaier, Lam Dai Vu, Evan Murphy, Amaya Vilches Barro, Kamal Swarup, Kamaljit Moirangthem, Bodil Jørgensen, Brigitte Van De Cotte, Tatsuaki Goh, Zhefeng Lin, Ute Voβ, Tom Beeckman, Malcolm J. Bennett, Kris Gevaert, Alexis Maizel, Ive De Smet^*

^*Corresponding author for this work

Section for Plant Glycobiology

23 Citations (Scopus)

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Abstract

In plants, postembryonic formation of new organs helps shape the adult organism. This requires the tight regulation of when and where a new organ is formed and a coordination of the underlying cell divisions. To build a root system, new lateral roots are continuously developing, and this process requires the tight coordination of asymmetric cell division in adjacent pericycle cells. We identified EXPANSIN A1 (EXPA1) as a cell wall modifying enzyme controlling the divisions marking lateral root initiation. Loss of EXPA1 leads to defects in the first asymmetric pericycle cell divisions and the radial swelling of the pericycle during auxin-driven lateral root formation. We conclude that a localized radial expansion of adjacent pericycle cells is required to position the asymmetric cell divisions and generate a core of small daughter cells, which is a prerequisite for lateral root organogenesis.

Original language	English
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	116
Issue number	17
Pages (from-to)	8597-8602
Number of pages	6
ISSN	0027-8424
DOIs	https://doi.org/10.1073/pnas.1820882116
Publication status	Published - 23 Apr 2019

Keywords

Arabidopsis thaliana
Cell wall remodeling
EXPANSIN A1
Lateral root development
Radial cell expansion

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EXPANSIN A1-mediated radial swelling of pericycle cells positions anticlinal cell divisions during lateral root initiationFinal published version, 1.26 MBLicence: CC BY-NC-ND

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Ramakrishna, P., Duarte, P. R., Rance, G. A., Schubert, M., Vordermaier, V., Vu, L. D., Murphy, E., Barro, A. V., Swarup, K., Moirangthem, K., Jørgensen, B., Van De Cotte, B., Goh, T., Lin, Z., Voβ, U., Beeckman, T., Bennett, M. J., Gevaert, K., Maizel, A., & De Smet, I. (2019). EXPANSIN A1-mediated radial swelling of pericycle cells positions anticlinal cell divisions during lateral root initiation. Proceedings of the National Academy of Sciences of the United States of America, 116(17), 8597-8602. https://doi.org/10.1073/pnas.1820882116

EXPANSIN A1-mediated radial swelling of pericycle cells positions anticlinal cell divisions during lateral root initiation. / Ramakrishna, Priya; Duarte, Paola Ruiz; Rance, Graham A. et al.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 116, No. 17, 23.04.2019, p. 8597-8602.

Research output: Contribution to journal › Journal article › Research › peer-review

Ramakrishna, P, Duarte, PR, Rance, GA, Schubert, M, Vordermaier, V, Vu, LD, Murphy, E, Barro, AV, Swarup, K, Moirangthem, K, Jørgensen, B, Van De Cotte, B, Goh, T, Lin, Z, Voβ, U, Beeckman, T, Bennett, MJ, Gevaert, K, Maizel, A & De Smet, I 2019, 'EXPANSIN A1-mediated radial swelling of pericycle cells positions anticlinal cell divisions during lateral root initiation', Proceedings of the National Academy of Sciences of the United States of America, vol. 116, no. 17, pp. 8597-8602. https://doi.org/10.1073/pnas.1820882116

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abstract = "In plants, postembryonic formation of new organs helps shape the adult organism. This requires the tight regulation of when and where a new organ is formed and a coordination of the underlying cell divisions. To build a root system, new lateral roots are continuously developing, and this process requires the tight coordination of asymmetric cell division in adjacent pericycle cells. We identified EXPANSIN A1 (EXPA1) as a cell wall modifying enzyme controlling the divisions marking lateral root initiation. Loss of EXPA1 leads to defects in the first asymmetric pericycle cell divisions and the radial swelling of the pericycle during auxin-driven lateral root formation. We conclude that a localized radial expansion of adjacent pericycle cells is required to position the asymmetric cell divisions and generate a core of small daughter cells, which is a prerequisite for lateral root organogenesis.",

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AU - Schubert, Martin

AU - Vordermaier, Vera

AU - Vu, Lam Dai

AU - Murphy, Evan

AU - Barro, Amaya Vilches

AU - Swarup, Kamal

AU - Moirangthem, Kamaljit

AU - Jørgensen, Bodil

AU - Van De Cotte, Brigitte

AU - Goh, Tatsuaki

AU - Lin, Zhefeng

AU - Voβ, Ute

AU - Beeckman, Tom

AU - Bennett, Malcolm J.

AU - Gevaert, Kris

AU - Maizel, Alexis

AU - De Smet, Ive

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N2 - In plants, postembryonic formation of new organs helps shape the adult organism. This requires the tight regulation of when and where a new organ is formed and a coordination of the underlying cell divisions. To build a root system, new lateral roots are continuously developing, and this process requires the tight coordination of asymmetric cell division in adjacent pericycle cells. We identified EXPANSIN A1 (EXPA1) as a cell wall modifying enzyme controlling the divisions marking lateral root initiation. Loss of EXPA1 leads to defects in the first asymmetric pericycle cell divisions and the radial swelling of the pericycle during auxin-driven lateral root formation. We conclude that a localized radial expansion of adjacent pericycle cells is required to position the asymmetric cell divisions and generate a core of small daughter cells, which is a prerequisite for lateral root organogenesis.

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KW - Cell wall remodeling

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EXPANSIN A1-mediated radial swelling of pericycle cells positions anticlinal cell divisions during lateral root initiation

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Keywords

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