Exercise-induced pyruvate dehydrogenase activation is not affected by 7 days of bed rest

Kristian Kiilerich; Stine Ringholm Jørgensen; Rasmus Sjørup Biensø; James P Fisher; Ninna Iversen; Gerrit van Hall; Jørgen Wojtaszewski; Bengt Saltin; Carsten Lundby; Jose A L Calbet; Henriette Pilegaard

doi:10.1152/japplphysiol.00063.2011

Exercise-induced pyruvate dehydrogenase activation is not affected by 7 days of bed rest

Kristian Kiilerich, Stine Ringholm Jørgensen, Rasmus Sjørup Biensø, James P Fisher, Ninna Iversen, Gerrit van Hall, Jørgen Wojtaszewski, Bengt Saltin, Carsten Lundby, Jose A L Calbet, Henriette Pilegaard

15 Citations (Scopus)

Abstract

To test the hypothesis that physical inactivity impairs the exercise-induced modulation of pyruvate dehydrogenase (PDH), six healthy normally physically active male subjects completed 7 days of bed rest. Before and immediately after the bed rest, the subjects completed an oral glucose tolerance test (OGTT) and a one-legged knee extensor exercise bout [45 min at 60% maximal load (W _max)] with muscle biopsies obtained from vastus lateralis before, immediately after exercise, and at 3 h of recovery. Blood samples were taken from the femoral vein and artery before and after 40 min of exercise. Glucose intake elicited a larger (P≤0.05) insulin response after bed rest than before, indicating glucose intolerance. There were no differences in lactate release/uptake across the exercising muscle before and after bed rest, but glucose uptake after 40 min of exercise was larger (P ≤ 0.05) before bed rest than after. Muscle glycogen content tended to be higher (0.05≤ P ≤ 0.10) after bed rest than before, but muscle glycogen breakdown in response to exercise was similar before and after bed rest. PDH-E1α protein content did not change in response to bed rest or in response to the exercise intervention. Exercise increased (P ≤ 0.05) the activity of PDH in the active form (PDHa) and induced (P ≤ 0.05) dephosphorylation of PDH-E1α on Ser ₂₉₃, Ser ₂₉₅ and Ser ₃₀₀, with no difference before and after bed rest. In conclusion, although 7 days of bed rest induced whole body glucose intolerance, exercise-induced PDH regulation in skeletal muscle was not changed. This suggests that exercise- induced PDH regulation in skeletal muscle is maintained in glucoseintolerant (e.g., insulin resistant) individuals.

Original language	English
Journal	Journal of Applied Physiology
Volume	111
Issue number	3
Pages (from-to)	751-757
Number of pages	7
ISSN	8750-7587
DOIs	https://doi.org/10.1152/japplphysiol.00063.2011
Publication status	Published - Sept 2011

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10.1152/japplphysiol.00063.2011

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title = "Exercise-induced pyruvate dehydrogenase activation is not affected by 7 days of bed rest",

abstract = "To test the hypothesis that physical inactivity impairs the exercise-induced modulation of pyruvate dehydrogenase (PDH), six healthy normally physically active male subjects completed 7 days of bed rest. Before and immediately after the bed rest, the subjects completed an oral glucose tolerance test (OGTT) and a one-legged knee extensor exercise bout [45 min at 60% maximal load (W max)] with muscle biopsies obtained from vastus lateralis before, immediately after exercise, and at 3 h of recovery. Blood samples were taken from the femoral vein and artery before and after 40 min of exercise. Glucose intake elicited a larger (P≤0.05) insulin response after bed rest than before, indicating glucose intolerance. There were no differences in lactate release/uptake across the exercising muscle before and after bed rest, but glucose uptake after 40 min of exercise was larger (P ≤ 0.05) before bed rest than after. Muscle glycogen content tended to be higher (0.05≤ P ≤ 0.10) after bed rest than before, but muscle glycogen breakdown in response to exercise was similar before and after bed rest. PDH-E1α protein content did not change in response to bed rest or in response to the exercise intervention. Exercise increased (P ≤ 0.05) the activity of PDH in the active form (PDHa) and induced (P ≤ 0.05) dephosphorylation of PDH-E1α on Ser 293, Ser 295 and Ser 300, with no difference before and after bed rest. In conclusion, although 7 days of bed rest induced whole body glucose intolerance, exercise-induced PDH regulation in skeletal muscle was not changed. This suggests that exercise- induced PDH regulation in skeletal muscle is maintained in glucoseintolerant (e.g., insulin resistant) individuals.",

author = "Kristian Kiilerich and J{\o}rgensen, {Stine Ringholm} and Biens{\o}, {Rasmus Sj{\o}rup} and Fisher, {James P} and Ninna Iversen and {van Hall}, Gerrit and J{\o}rgen Wojtaszewski and Bengt Saltin and Carsten Lundby and Calbet, {Jose A L} and Henriette Pilegaard",

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doi = "10.1152/japplphysiol.00063.2011",

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T1 - Exercise-induced pyruvate dehydrogenase activation is not affected by 7 days of bed rest

AU - Kiilerich, Kristian

AU - Jørgensen, Stine Ringholm

AU - Biensø, Rasmus Sjørup

AU - Fisher, James P

AU - Iversen, Ninna

AU - van Hall, Gerrit

AU - Wojtaszewski, Jørgen

AU - Saltin, Bengt

AU - Lundby, Carsten

AU - Calbet, Jose A L

AU - Pilegaard, Henriette

N1 - CURIS 2011 5200 067

PY - 2011/9

Y1 - 2011/9

N2 - To test the hypothesis that physical inactivity impairs the exercise-induced modulation of pyruvate dehydrogenase (PDH), six healthy normally physically active male subjects completed 7 days of bed rest. Before and immediately after the bed rest, the subjects completed an oral glucose tolerance test (OGTT) and a one-legged knee extensor exercise bout [45 min at 60% maximal load (W max)] with muscle biopsies obtained from vastus lateralis before, immediately after exercise, and at 3 h of recovery. Blood samples were taken from the femoral vein and artery before and after 40 min of exercise. Glucose intake elicited a larger (P≤0.05) insulin response after bed rest than before, indicating glucose intolerance. There were no differences in lactate release/uptake across the exercising muscle before and after bed rest, but glucose uptake after 40 min of exercise was larger (P ≤ 0.05) before bed rest than after. Muscle glycogen content tended to be higher (0.05≤ P ≤ 0.10) after bed rest than before, but muscle glycogen breakdown in response to exercise was similar before and after bed rest. PDH-E1α protein content did not change in response to bed rest or in response to the exercise intervention. Exercise increased (P ≤ 0.05) the activity of PDH in the active form (PDHa) and induced (P ≤ 0.05) dephosphorylation of PDH-E1α on Ser 293, Ser 295 and Ser 300, with no difference before and after bed rest. In conclusion, although 7 days of bed rest induced whole body glucose intolerance, exercise-induced PDH regulation in skeletal muscle was not changed. This suggests that exercise- induced PDH regulation in skeletal muscle is maintained in glucoseintolerant (e.g., insulin resistant) individuals.

AB - To test the hypothesis that physical inactivity impairs the exercise-induced modulation of pyruvate dehydrogenase (PDH), six healthy normally physically active male subjects completed 7 days of bed rest. Before and immediately after the bed rest, the subjects completed an oral glucose tolerance test (OGTT) and a one-legged knee extensor exercise bout [45 min at 60% maximal load (W max)] with muscle biopsies obtained from vastus lateralis before, immediately after exercise, and at 3 h of recovery. Blood samples were taken from the femoral vein and artery before and after 40 min of exercise. Glucose intake elicited a larger (P≤0.05) insulin response after bed rest than before, indicating glucose intolerance. There were no differences in lactate release/uptake across the exercising muscle before and after bed rest, but glucose uptake after 40 min of exercise was larger (P ≤ 0.05) before bed rest than after. Muscle glycogen content tended to be higher (0.05≤ P ≤ 0.10) after bed rest than before, but muscle glycogen breakdown in response to exercise was similar before and after bed rest. PDH-E1α protein content did not change in response to bed rest or in response to the exercise intervention. Exercise increased (P ≤ 0.05) the activity of PDH in the active form (PDHa) and induced (P ≤ 0.05) dephosphorylation of PDH-E1α on Ser 293, Ser 295 and Ser 300, with no difference before and after bed rest. In conclusion, although 7 days of bed rest induced whole body glucose intolerance, exercise-induced PDH regulation in skeletal muscle was not changed. This suggests that exercise- induced PDH regulation in skeletal muscle is maintained in glucoseintolerant (e.g., insulin resistant) individuals.

U2 - 10.1152/japplphysiol.00063.2011

DO - 10.1152/japplphysiol.00063.2011

M3 - Journal article

C2 - 21680880

SN - 8750-7587

VL - 111

SP - 751

EP - 757

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

IS - 3

ER -

Exercise-induced pyruvate dehydrogenase activation is not affected by 7 days of bed rest

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