Evidence of the production of galactooligosaccharide from whey permeate by the microalgae Tetradesmus obliquus

Shyam Suwal; Jihed Bentahar; Alice Marciniak; Lucie Beaulieu; Jean Sébastien Deschênes; Alain Doyen

doi:10.1016/j.algal.2019.101470

Evidence of the production of galactooligosaccharide from whey permeate by the microalgae Tetradesmus obliquus

Shyam Suwal, Jihed Bentahar, Alice Marciniak, Lucie Beaulieu, Jean Sébastien Deschênes, Alain Doyen^*

^*Corresponding author for this work

3 Citations (Scopus)

Abstract

Galactooligosaccharides (GOS) are prebiotics commonly produced from lactose via an enzymatic reaction catalyzed by β-galactosidase (β-gal) which is present in many organisms, including microalgae. Whey permeate (WP), a major byproduct of the dairy industry rich in lactose, represents an interesting medium for producing GOS as high value-added ingredients. Using the microalgae Tetradesmus obliquus as the enzyme source could be an interesting alternative due to its lower nutritional requirements for growth and its applicability in industry. Therefore, this study investigates the growth of T. obliquus and its potential to produce GOS in WP compared to modified Bold's Basal medium (BBM). The growth kinetics, β-gal activity, and medium composition, such as minerals, lactose, glucose, galactose and GOS concentrations, were evaluated during algal cultivation for up to 21 days. The growth rate of T. obliquus was significantly higher in WP, reaching a biomass concentration 4.5 times higher than when growing in BBM. The growth stimulation in WP is directly proportional to the concentration of minerals and ionic species in WP compared to BBM, except for nitrate. The lactose concentration decreased significantly (by 21%) in WP only, with substantial production of the simple carbohydrates, glucose (3.1 g/L) and galactose (4.1 g/L). The higher degree of lactose hydrolysis in WP was supported by significantly higher β-gal specific activity and volumetric productivity. In addition, we detected, for the first time, the production of a trisaccharide GOS in WP whose concentration increased with time, reaching 4.4 g/L and conversion yield of 8.4% (w/w of initial lactose) after 21 days. Overall, these results hold promise for developing a novel bioprocess for the production of value-added products from WP, but the microalgal enzyme must first be characterized and optimized.

Original language	English
Article number	101470
Journal	Algal Research
Volume	39
Pages (from-to)	1-7
ISSN	2211-9264
DOIs	https://doi.org/10.1016/j.algal.2019.101470
Publication status	Published - 2019

Keywords

Galactooligosaccharide
Lactose
Microalgae
Whey permeate
β-galactosidase

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10.1016/j.algal.2019.101470

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title = "Evidence of the production of galactooligosaccharide from whey permeate by the microalgae Tetradesmus obliquus",

abstract = "Galactooligosaccharides (GOS) are prebiotics commonly produced from lactose via an enzymatic reaction catalyzed by β-galactosidase (β-gal) which is present in many organisms, including microalgae. Whey permeate (WP), a major byproduct of the dairy industry rich in lactose, represents an interesting medium for producing GOS as high value-added ingredients. Using the microalgae Tetradesmus obliquus as the enzyme source could be an interesting alternative due to its lower nutritional requirements for growth and its applicability in industry. Therefore, this study investigates the growth of T. obliquus and its potential to produce GOS in WP compared to modified Bold's Basal medium (BBM). The growth kinetics, β-gal activity, and medium composition, such as minerals, lactose, glucose, galactose and GOS concentrations, were evaluated during algal cultivation for up to 21 days. The growth rate of T. obliquus was significantly higher in WP, reaching a biomass concentration 4.5 times higher than when growing in BBM. The growth stimulation in WP is directly proportional to the concentration of minerals and ionic species in WP compared to BBM, except for nitrate. The lactose concentration decreased significantly (by 21%) in WP only, with substantial production of the simple carbohydrates, glucose (3.1 g/L) and galactose (4.1 g/L). The higher degree of lactose hydrolysis in WP was supported by significantly higher β-gal specific activity and volumetric productivity. In addition, we detected, for the first time, the production of a trisaccharide GOS in WP whose concentration increased with time, reaching 4.4 g/L and conversion yield of 8.4% (w/w of initial lactose) after 21 days. Overall, these results hold promise for developing a novel bioprocess for the production of value-added products from WP, but the microalgal enzyme must first be characterized and optimized.",

keywords = "Galactooligosaccharide, Lactose, Microalgae, Whey permeate, β-galactosidase",

author = "Shyam Suwal and Jihed Bentahar and Alice Marciniak and Lucie Beaulieu and Desch{\^e}nes, {Jean S{\'e}bastien} and Alain Doyen",

year = "2019",

doi = "10.1016/j.algal.2019.101470",

language = "English",

volume = "39",

pages = "1--7",

journal = "Algal Research",

issn = "2211-9264",

publisher = "Elsevier",

}

TY - JOUR

T1 - Evidence of the production of galactooligosaccharide from whey permeate by the microalgae Tetradesmus obliquus

AU - Suwal, Shyam

AU - Bentahar, Jihed

AU - Marciniak, Alice

AU - Beaulieu, Lucie

AU - Deschênes, Jean Sébastien

AU - Doyen, Alain

PY - 2019

Y1 - 2019

N2 - Galactooligosaccharides (GOS) are prebiotics commonly produced from lactose via an enzymatic reaction catalyzed by β-galactosidase (β-gal) which is present in many organisms, including microalgae. Whey permeate (WP), a major byproduct of the dairy industry rich in lactose, represents an interesting medium for producing GOS as high value-added ingredients. Using the microalgae Tetradesmus obliquus as the enzyme source could be an interesting alternative due to its lower nutritional requirements for growth and its applicability in industry. Therefore, this study investigates the growth of T. obliquus and its potential to produce GOS in WP compared to modified Bold's Basal medium (BBM). The growth kinetics, β-gal activity, and medium composition, such as minerals, lactose, glucose, galactose and GOS concentrations, were evaluated during algal cultivation for up to 21 days. The growth rate of T. obliquus was significantly higher in WP, reaching a biomass concentration 4.5 times higher than when growing in BBM. The growth stimulation in WP is directly proportional to the concentration of minerals and ionic species in WP compared to BBM, except for nitrate. The lactose concentration decreased significantly (by 21%) in WP only, with substantial production of the simple carbohydrates, glucose (3.1 g/L) and galactose (4.1 g/L). The higher degree of lactose hydrolysis in WP was supported by significantly higher β-gal specific activity and volumetric productivity. In addition, we detected, for the first time, the production of a trisaccharide GOS in WP whose concentration increased with time, reaching 4.4 g/L and conversion yield of 8.4% (w/w of initial lactose) after 21 days. Overall, these results hold promise for developing a novel bioprocess for the production of value-added products from WP, but the microalgal enzyme must first be characterized and optimized.

AB - Galactooligosaccharides (GOS) are prebiotics commonly produced from lactose via an enzymatic reaction catalyzed by β-galactosidase (β-gal) which is present in many organisms, including microalgae. Whey permeate (WP), a major byproduct of the dairy industry rich in lactose, represents an interesting medium for producing GOS as high value-added ingredients. Using the microalgae Tetradesmus obliquus as the enzyme source could be an interesting alternative due to its lower nutritional requirements for growth and its applicability in industry. Therefore, this study investigates the growth of T. obliquus and its potential to produce GOS in WP compared to modified Bold's Basal medium (BBM). The growth kinetics, β-gal activity, and medium composition, such as minerals, lactose, glucose, galactose and GOS concentrations, were evaluated during algal cultivation for up to 21 days. The growth rate of T. obliquus was significantly higher in WP, reaching a biomass concentration 4.5 times higher than when growing in BBM. The growth stimulation in WP is directly proportional to the concentration of minerals and ionic species in WP compared to BBM, except for nitrate. The lactose concentration decreased significantly (by 21%) in WP only, with substantial production of the simple carbohydrates, glucose (3.1 g/L) and galactose (4.1 g/L). The higher degree of lactose hydrolysis in WP was supported by significantly higher β-gal specific activity and volumetric productivity. In addition, we detected, for the first time, the production of a trisaccharide GOS in WP whose concentration increased with time, reaching 4.4 g/L and conversion yield of 8.4% (w/w of initial lactose) after 21 days. Overall, these results hold promise for developing a novel bioprocess for the production of value-added products from WP, but the microalgal enzyme must first be characterized and optimized.

KW - Galactooligosaccharide

KW - Lactose

KW - Microalgae

KW - Whey permeate

KW - β-galactosidase

U2 - 10.1016/j.algal.2019.101470

DO - 10.1016/j.algal.2019.101470

M3 - Journal article

AN - SCOPUS:85062985743

SN - 2211-9264

VL - 39

SP - 1

EP - 7

JO - Algal Research

JF - Algal Research

M1 - 101470

ER -

Evidence of the production of galactooligosaccharide from whey permeate by the microalgae Tetradesmus obliquus

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Keywords

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