Evaluation of the systemic acute phase response and endometrial gene expression of serum amyloid A and pro- and anti-inflammatory cytokines in mares with experimentally induced endometritis

Mette Christoffersen; Camilla Dooleweerdt Baagø; Stine Jacobsen; Anders Miki Bojesen; Morten Rønn Petersen; Henrik Lehn-Jensen

doi:10.1016/j.vetimm.2010.07.011

Evaluation of the systemic acute phase response and endometrial gene expression of serum amyloid A and pro- and anti-inflammatory cytokines in mares with experimentally induced endometritis

Mette Christoffersen, Camilla Dooleweerdt Baagø, Stine Jacobsen, Anders Miki Bojesen, Morten Rønn Petersen, Henrik Lehn-Jensen

53 Citations (Scopus)

Abstract

Infectious infertility in the mare is clinically well described, little is however known about the systemic acute phase reaction (APR) and local immunological responses accompanying equine endometritis. The aim of this study was to monitor selected markers of the APR in the systemic circulation and to correlate them to the local innate immune response in the uterus during infectious endometritis. Six adult standard bred mares received an intrauterine infusion of 10⁹CFU Escherichia coli. Blood samples were obtained before (0h) and 3, 6, 12, 24, 36, 48, 72, 96 and 120h post inoculation (pi), and endometrial biopsies were sampled before, and 3, 12, 24, 48 and 72h pi. The infectious endometritis elicited a systemic APR with significantly increased concentrations of the acute phase proteins (APPs) serum amyloid A (SAA) and fibrinogen. Relative gene expression analyses were performed on extracted RNA from endometrial biopsies using quantitative real-time PCR and specific primers for SAA and pro- and anti-inflammatory cytokines. Expression of SAA was significantly up-regulated at 3 and 12h pi, and a significant up-regulated expression of IL-1β, TNFα, IL-8 and IL-10 was observed at 3h pi. Plasma concentration of SAA was significantly correlated to endometrial SAA expression. The results of the present study demonstrate that endometritis gives rise to a systemic APR and an up-regulated endometrial gene expression of SAA and several pro-and anti-inflammatory cytokines. Understanding endometrial expression of acute phase proteins and selected cytokines contributing to uterine immunity in equine endometritis could improve understanding of events leading to infertility in the mare and help identify candidate genes of mediators/markers for diagnostic use.

Original language	English
Journal	Veterinary Immunology and Immunopathology
Volume	138
Issue number	1-2
Pages (from-to)	95-105
Number of pages	11
ISSN	0165-2427
DOIs	https://doi.org/10.1016/j.vetimm.2010.07.011
Publication status	Published - 15 Nov 2010

Keywords

Former LIFE faculty

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10.1016/j.vetimm.2010.07.011

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Christoffersen, M., Baagø, C. D., Jacobsen, S., Bojesen, A. M., Petersen, M. R., & Lehn-Jensen, H. (2010). Evaluation of the systemic acute phase response and endometrial gene expression of serum amyloid A and pro- and anti-inflammatory cytokines in mares with experimentally induced endometritis. Veterinary Immunology and Immunopathology, 138(1-2), 95-105. https://doi.org/10.1016/j.vetimm.2010.07.011

Evaluation of the systemic acute phase response and endometrial gene expression of serum amyloid A and pro- and anti-inflammatory cytokines in mares with experimentally induced endometritis. / Christoffersen, Mette; Baagø, Camilla Dooleweerdt; Jacobsen, Stine et al.
In: Veterinary Immunology and Immunopathology, Vol. 138, No. 1-2, 15.11.2010, p. 95-105.

Research output: Contribution to journal › Journal article › Research › peer-review

Christoffersen, M, Baagø, CD, Jacobsen, S , Bojesen, AM, Petersen, MR & Lehn-Jensen, H 2010, 'Evaluation of the systemic acute phase response and endometrial gene expression of serum amyloid A and pro- and anti-inflammatory cytokines in mares with experimentally induced endometritis', Veterinary Immunology and Immunopathology, vol. 138, no. 1-2, pp. 95-105. https://doi.org/10.1016/j.vetimm.2010.07.011

Christoffersen M, Baagø CD, Jacobsen S , Bojesen AM, Petersen MR, Lehn-Jensen H. Evaluation of the systemic acute phase response and endometrial gene expression of serum amyloid A and pro- and anti-inflammatory cytokines in mares with experimentally induced endometritis. Veterinary Immunology and Immunopathology. 2010 Nov 15;138(1-2):95-105. doi: 10.1016/j.vetimm.2010.07.011

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abstract = "Infectious infertility in the mare is clinically well described, little is however known about the systemic acute phase reaction (APR) and local immunological responses accompanying equine endometritis. The aim of this study was to monitor selected markers of the APR in the systemic circulation and to correlate them to the local innate immune response in the uterus during infectious endometritis. Six adult standard bred mares received an intrauterine infusion of 109CFU Escherichia coli. Blood samples were obtained before (0h) and 3, 6, 12, 24, 36, 48, 72, 96 and 120h post inoculation (pi), and endometrial biopsies were sampled before, and 3, 12, 24, 48 and 72h pi. The infectious endometritis elicited a systemic APR with significantly increased concentrations of the acute phase proteins (APPs) serum amyloid A (SAA) and fibrinogen. Relative gene expression analyses were performed on extracted RNA from endometrial biopsies using quantitative real-time PCR and specific primers for SAA and pro- and anti-inflammatory cytokines. Expression of SAA was significantly up-regulated at 3 and 12h pi, and a significant up-regulated expression of IL-1β, TNFα, IL-8 and IL-10 was observed at 3h pi. Plasma concentration of SAA was significantly correlated to endometrial SAA expression. The results of the present study demonstrate that endometritis gives rise to a systemic APR and an up-regulated endometrial gene expression of SAA and several pro-and anti-inflammatory cytokines. Understanding endometrial expression of acute phase proteins and selected cytokines contributing to uterine immunity in equine endometritis could improve understanding of events leading to infertility in the mare and help identify candidate genes of mediators/markers for diagnostic use.",

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author = "Mette Christoffersen and Baag{\o}, {Camilla Dooleweerdt} and Stine Jacobsen and Bojesen, {Anders Miki} and Petersen, {Morten R{\o}nn} and Henrik Lehn-Jensen",

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doi = "10.1016/j.vetimm.2010.07.011",

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AU - Christoffersen, Mette

AU - Baagø, Camilla Dooleweerdt

AU - Jacobsen, Stine

AU - Bojesen, Anders Miki

AU - Petersen, Morten Rønn

AU - Lehn-Jensen, Henrik

PY - 2010/11/15

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N2 - Infectious infertility in the mare is clinically well described, little is however known about the systemic acute phase reaction (APR) and local immunological responses accompanying equine endometritis. The aim of this study was to monitor selected markers of the APR in the systemic circulation and to correlate them to the local innate immune response in the uterus during infectious endometritis. Six adult standard bred mares received an intrauterine infusion of 109CFU Escherichia coli. Blood samples were obtained before (0h) and 3, 6, 12, 24, 36, 48, 72, 96 and 120h post inoculation (pi), and endometrial biopsies were sampled before, and 3, 12, 24, 48 and 72h pi. The infectious endometritis elicited a systemic APR with significantly increased concentrations of the acute phase proteins (APPs) serum amyloid A (SAA) and fibrinogen. Relative gene expression analyses were performed on extracted RNA from endometrial biopsies using quantitative real-time PCR and specific primers for SAA and pro- and anti-inflammatory cytokines. Expression of SAA was significantly up-regulated at 3 and 12h pi, and a significant up-regulated expression of IL-1β, TNFα, IL-8 and IL-10 was observed at 3h pi. Plasma concentration of SAA was significantly correlated to endometrial SAA expression. The results of the present study demonstrate that endometritis gives rise to a systemic APR and an up-regulated endometrial gene expression of SAA and several pro-and anti-inflammatory cytokines. Understanding endometrial expression of acute phase proteins and selected cytokines contributing to uterine immunity in equine endometritis could improve understanding of events leading to infertility in the mare and help identify candidate genes of mediators/markers for diagnostic use.

AB - Infectious infertility in the mare is clinically well described, little is however known about the systemic acute phase reaction (APR) and local immunological responses accompanying equine endometritis. The aim of this study was to monitor selected markers of the APR in the systemic circulation and to correlate them to the local innate immune response in the uterus during infectious endometritis. Six adult standard bred mares received an intrauterine infusion of 109CFU Escherichia coli. Blood samples were obtained before (0h) and 3, 6, 12, 24, 36, 48, 72, 96 and 120h post inoculation (pi), and endometrial biopsies were sampled before, and 3, 12, 24, 48 and 72h pi. The infectious endometritis elicited a systemic APR with significantly increased concentrations of the acute phase proteins (APPs) serum amyloid A (SAA) and fibrinogen. Relative gene expression analyses were performed on extracted RNA from endometrial biopsies using quantitative real-time PCR and specific primers for SAA and pro- and anti-inflammatory cytokines. Expression of SAA was significantly up-regulated at 3 and 12h pi, and a significant up-regulated expression of IL-1β, TNFα, IL-8 and IL-10 was observed at 3h pi. Plasma concentration of SAA was significantly correlated to endometrial SAA expression. The results of the present study demonstrate that endometritis gives rise to a systemic APR and an up-regulated endometrial gene expression of SAA and several pro-and anti-inflammatory cytokines. Understanding endometrial expression of acute phase proteins and selected cytokines contributing to uterine immunity in equine endometritis could improve understanding of events leading to infertility in the mare and help identify candidate genes of mediators/markers for diagnostic use.

KW - Former LIFE faculty

KW - Endometritis

KW - Escherichia coli

KW - Serum amyloid A

KW - Cytokine

KW - Horse

U2 - 10.1016/j.vetimm.2010.07.011

DO - 10.1016/j.vetimm.2010.07.011

M3 - Journal article

C2 - 20728224

SN - 0165-2427

VL - 138

SP - 95

EP - 105

JO - Veterinary Immunology and Immunopathology

JF - Veterinary Immunology and Immunopathology

IS - 1-2

ER -

Evaluation of the systemic acute phase response and endometrial gene expression of serum amyloid A and pro- and anti-inflammatory cytokines in mares with experimentally induced endometritis

Abstract

Keywords

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