TY - JOUR
T1 - Evaluation of synthetic promoters in Physcomitrella patens
AU - Peramuna, Anantha
AU - Bae, Hansol
AU - Rasmussen, Erling Koch
AU - Dueholm, Bjørn
AU - Waibel, Thomas
AU - Critchley, Joanna H.
AU - Brzezek, Kerstin
AU - Roberts, Michael
AU - Simonsen, Henrik Toft
PY - 2018/6/2
Y1 - 2018/6/2
N2 - Securing a molecular toolbox including diverse promoters is essential for genome engineering. However, native promoters have limitations such as the available number or the length of the promoter. In this work, three short synthetic promoters were characterized by using the yellow fluorescent protein Venus. All of the tested promoters were active and showed higher mRNA expression than housekeeping gene PpAct7, and similar protein expression level to the AtUBQ10 promoter. This study shows that few cis-regulatory elements are enough to establish a strong promoter for continuous expression of genes in plants. Along with this, the study enhance the number of available promotors to be used in P. patens. It also demonstrates the potential to construct multiple non-native promoters on demand, which would aid to resolve one bottleneck in multiple pathway expression in P. patens and other plants.
AB - Securing a molecular toolbox including diverse promoters is essential for genome engineering. However, native promoters have limitations such as the available number or the length of the promoter. In this work, three short synthetic promoters were characterized by using the yellow fluorescent protein Venus. All of the tested promoters were active and showed higher mRNA expression than housekeeping gene PpAct7, and similar protein expression level to the AtUBQ10 promoter. This study shows that few cis-regulatory elements are enough to establish a strong promoter for continuous expression of genes in plants. Along with this, the study enhance the number of available promotors to be used in P. patens. It also demonstrates the potential to construct multiple non-native promoters on demand, which would aid to resolve one bottleneck in multiple pathway expression in P. patens and other plants.
KW - Actin promoter
KW - Physcomitrella patens
KW - Synthetic biology
KW - Synthetic promoters
KW - Ubiquitin promoter
KW - Venus
UR - http://www.scopus.com/inward/record.url?scp=85045703059&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2018.04.092
DO - 10.1016/j.bbrc.2018.04.092
M3 - Journal article
C2 - 29660341
AN - SCOPUS:85045703059
SN - 0006-291X
VL - 500
SP - 418
EP - 422
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -