Evaluation of simple tests for detection of HIV antibodies: analysis of interobserver variation in Tanzania

A Jørgensen; Jing Shao; S Maselle; E Yangi; A Thomsen; S Matunda; Ib Christian Bygbjerg; P Gøtzsche; J Svendsen; null Skinhøj

Evaluation of simple tests for detection of HIV antibodies: analysis of interobserver variation in Tanzania

A Jørgensen, Jing Shao, S Maselle, E Yangi, A Thomsen, S Matunda, Ib Christian Bygbjerg, P Gøtzsche, J Svendsen, Skinhøj

Department of Immunology and Microbiology

1 Citation (Scopus)

Abstract

200 sera were tested for HIV antibodies with different tests in Tanzania. The results were interpreted by 5 different observers with different laboratory experience. There was considerable variation between observers and between testing methods. HIV-Chek was easiest to perform with little interobserver variation, but a few probably false negative readings were seen. Serodia gave many false positives. Western Blots gave more diverging results due to indeterminate sera and lack of training. HIV-Chek seems best when time, facilities, and training are limited and if combined with Serodia false negative results can be excluded.

Original language	English
Journal	Scandinavian Journal of Infectious Diseases
Volume	22
Issue number	3
Pages (from-to)	283-5
Number of pages	3
ISSN	0036-5548
Publication status	Published - 1 Jan 1990

Keywords

Blotting, Western
Enzyme-Linked Immunosorbent Assay
False Negative Reactions
False Positive Reactions
HIV Antibodies
HIV Infections
Humans
Observer Variation
Predictive Value of Tests
Tanzania

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title = "Evaluation of simple tests for detection of HIV antibodies: analysis of interobserver variation in Tanzania",

abstract = "200 sera were tested for HIV antibodies with different tests in Tanzania. The results were interpreted by 5 different observers with different laboratory experience. There was considerable variation between observers and between testing methods. HIV-Chek was easiest to perform with little interobserver variation, but a few probably false negative readings were seen. Serodia gave many false positives. Western Blots gave more diverging results due to indeterminate sera and lack of training. HIV-Chek seems best when time, facilities, and training are limited and if combined with Serodia false negative results can be excluded.",

keywords = "Blotting, Western, Enzyme-Linked Immunosorbent Assay, False Negative Reactions, False Positive Reactions, HIV Antibodies, HIV Infections, Humans, Observer Variation, Predictive Value of Tests, Tanzania",

author = "A J{\o}rgensen and Jing Shao and S Maselle and E Yangi and A Thomsen and S Matunda and Bygbjerg, {Ib Christian} and P G{\o}tzsche and J Svendsen and Skinh{\o}j",

year = "1990",

month = jan,

day = "1",

language = "English",

volume = "22",

pages = "283--5",

journal = "Infectious Diseases",

issn = "2374-4235",

publisher = "Taylor & Francis",

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TY - JOUR

T1 - Evaluation of simple tests for detection of HIV antibodies: analysis of interobserver variation in Tanzania

AU - Jørgensen, A

AU - Shao, Jing

AU - Maselle, S

AU - Yangi, E

AU - Thomsen, A

AU - Matunda, S

AU - Bygbjerg, Ib Christian

AU - Gøtzsche, P

AU - Svendsen, J

AU - Skinhøj, null

PY - 1990/1/1

Y1 - 1990/1/1

N2 - 200 sera were tested for HIV antibodies with different tests in Tanzania. The results were interpreted by 5 different observers with different laboratory experience. There was considerable variation between observers and between testing methods. HIV-Chek was easiest to perform with little interobserver variation, but a few probably false negative readings were seen. Serodia gave many false positives. Western Blots gave more diverging results due to indeterminate sera and lack of training. HIV-Chek seems best when time, facilities, and training are limited and if combined with Serodia false negative results can be excluded.

AB - 200 sera were tested for HIV antibodies with different tests in Tanzania. The results were interpreted by 5 different observers with different laboratory experience. There was considerable variation between observers and between testing methods. HIV-Chek was easiest to perform with little interobserver variation, but a few probably false negative readings were seen. Serodia gave many false positives. Western Blots gave more diverging results due to indeterminate sera and lack of training. HIV-Chek seems best when time, facilities, and training are limited and if combined with Serodia false negative results can be excluded.

KW - Blotting, Western

KW - Enzyme-Linked Immunosorbent Assay

KW - False Negative Reactions

KW - False Positive Reactions

KW - HIV Antibodies

KW - HIV Infections

KW - Humans

KW - Observer Variation

KW - Predictive Value of Tests

KW - Tanzania

M3 - Journal article

C2 - 2371543

SN - 2374-4235

VL - 22

SP - 283

EP - 285

JO - Infectious Diseases

JF - Infectious Diseases

IS - 3

ER -

Evaluation of simple tests for detection of HIV antibodies: analysis of interobserver variation in Tanzania

Abstract

Keywords

UN SDGs

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