Evaluation of simple and inexpensive high-throughput methods for phytic acid determination

Victor Raboy; Amy Johnson; Kristin Bilyeu; Henrik Brinch-Pedersen; Karen Cichy; Richard F. Hurrell; Christophe Zeder; Søren Kjærsgaard Rasmussen; Tom D. Warkentin; Pushparajah Thavarajah; Jinrui Shi; Lan Zhou; Qingyao Shu

doi:10.1007/s11746-016-2946-y

Evaluation of simple and inexpensive high-throughput methods for phytic acid determination

Victor Raboy, Amy Johnson, Kristin Bilyeu, Henrik Brinch-Pedersen, Karen Cichy, Richard F. Hurrell, Christophe Zeder, Søren Kjærsgaard Rasmussen, Tom D. Warkentin, Pushparajah Thavarajah, Jinrui Shi, Lan Zhou, Qingyao Shu

Section for Plant and Soil Sciences

5 Citations (Scopus)

Abstract

High-throughput/low-cost/low-tech methods for phytic acid determination that are sufficiently accurate and reproducible would be of value in plant genetics, crop breeding and in the food and feed industries. Variants of two candidate methods, those described by Vaintraub and Lapteva (Anal Biochem 175:227–24, 1988; “VL” methods) and Huang and Lantzsch (J Sci Food Agric 34:1423–1426, 1983; “HL” methods), were evaluated. The primary concern with these methods is that, due to interference of matrix constituents including inorganic P, they can overestimate phytic acid and are ineffective at low levels of phytic acid. Twelve seed flours, representing lines of soybean, maize, barley and dry bean, containing a wide range of phytic acid levels, were analyzed by a minimum of eight cooperating laboratories using three variants of the VL method and two variants of the HL method. No method had consistently acceptable (˂2.0”) “Horwitz ratios”, a measure of reproducibility, although some treatments approached that. For example, one variant of the VL method when used to assay a soybean flour with a “standard” level of phytic acid had a Horwitz ratio of 2.15. Some variants of the VL method were adequate for analyses of cereal grains regardless of phytic acid level but none accurately measured phytic acid when at low levels in soybean flours. One variant of the HL method in which the 0.2 N HCl extraction media is modified to contain 10% Na₂SO₄, did accurately measure phytic acid levels in both cereal and legume flours regardless of endogenous phytic acid levels or matrix constituents.

Original language	English
Journal	Journal of the American Oil Chemists' Society
Volume	94
Issue number	3
Pages (from-to)	353-362
Number of pages	10
ISSN	0003-021X
DOIs	https://doi.org/10.1007/s11746-016-2946-y
Publication status	Published - 1 Mar 2017

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Raboy, V., Johnson, A., Bilyeu, K., Brinch-Pedersen, H., Cichy, K., Hurrell, R. F., Zeder, C., Rasmussen, S. K., Warkentin, T. D., Thavarajah, P., Shi, J., Zhou, L., & Shu, Q. (2017). Evaluation of simple and inexpensive high-throughput methods for phytic acid determination. Journal of the American Oil Chemists' Society, 94(3), 353-362. https://doi.org/10.1007/s11746-016-2946-y

Raboy, V, Johnson, A, Bilyeu, K, Brinch-Pedersen, H, Cichy, K, Hurrell, RF, Zeder, C, Rasmussen, SK, Warkentin, TD, Thavarajah, P, Shi, J, Zhou, L & Shu, Q 2017, 'Evaluation of simple and inexpensive high-throughput methods for phytic acid determination', Journal of the American Oil Chemists' Society, vol. 94, no. 3, pp. 353-362. https://doi.org/10.1007/s11746-016-2946-y

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abstract = "High-throughput/low-cost/low-tech methods for phytic acid determination that are sufficiently accurate and reproducible would be of value in plant genetics, crop breeding and in the food and feed industries. Variants of two candidate methods, those described by Vaintraub and Lapteva (Anal Biochem 175:227–24, 1988; “VL” methods) and Huang and Lantzsch (J Sci Food Agric 34:1423–1426, 1983; “HL” methods), were evaluated. The primary concern with these methods is that, due to interference of matrix constituents including inorganic P, they can overestimate phytic acid and are ineffective at low levels of phytic acid. Twelve seed flours, representing lines of soybean, maize, barley and dry bean, containing a wide range of phytic acid levels, were analyzed by a minimum of eight cooperating laboratories using three variants of the VL method and two variants of the HL method. No method had consistently acceptable (˂2.0”) “Horwitz ratios”, a measure of reproducibility, although some treatments approached that. For example, one variant of the VL method when used to assay a soybean flour with a “standard” level of phytic acid had a Horwitz ratio of 2.15. Some variants of the VL method were adequate for analyses of cereal grains regardless of phytic acid level but none accurately measured phytic acid when at low levels in soybean flours. One variant of the HL method in which the 0.2 N HCl extraction media is modified to contain 10% Na2SO4, did accurately measure phytic acid levels in both cereal and legume flours regardless of endogenous phytic acid levels or matrix constituents.",

author = "Victor Raboy and Amy Johnson and Kristin Bilyeu and Henrik Brinch-Pedersen and Karen Cichy and Hurrell, {Richard F.} and Christophe Zeder and Rasmussen, {S{\o}ren Kj{\ae}rsgaard} and Warkentin, {Tom D.} and Pushparajah Thavarajah and Jinrui Shi and Lan Zhou and Qingyao Shu",

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AU - Johnson, Amy

AU - Bilyeu, Kristin

AU - Brinch-Pedersen, Henrik

AU - Cichy, Karen

AU - Hurrell, Richard F.

AU - Zeder, Christophe

AU - Rasmussen, Søren Kjærsgaard

AU - Warkentin, Tom D.

AU - Thavarajah, Pushparajah

AU - Shi, Jinrui

AU - Zhou, Lan

AU - Shu, Qingyao

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AB - High-throughput/low-cost/low-tech methods for phytic acid determination that are sufficiently accurate and reproducible would be of value in plant genetics, crop breeding and in the food and feed industries. Variants of two candidate methods, those described by Vaintraub and Lapteva (Anal Biochem 175:227–24, 1988; “VL” methods) and Huang and Lantzsch (J Sci Food Agric 34:1423–1426, 1983; “HL” methods), were evaluated. The primary concern with these methods is that, due to interference of matrix constituents including inorganic P, they can overestimate phytic acid and are ineffective at low levels of phytic acid. Twelve seed flours, representing lines of soybean, maize, barley and dry bean, containing a wide range of phytic acid levels, were analyzed by a minimum of eight cooperating laboratories using three variants of the VL method and two variants of the HL method. No method had consistently acceptable (˂2.0”) “Horwitz ratios”, a measure of reproducibility, although some treatments approached that. For example, one variant of the VL method when used to assay a soybean flour with a “standard” level of phytic acid had a Horwitz ratio of 2.15. Some variants of the VL method were adequate for analyses of cereal grains regardless of phytic acid level but none accurately measured phytic acid when at low levels in soybean flours. One variant of the HL method in which the 0.2 N HCl extraction media is modified to contain 10% Na2SO4, did accurately measure phytic acid levels in both cereal and legume flours regardless of endogenous phytic acid levels or matrix constituents.

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JO - Oil & Soap

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ER -

Evaluation of simple and inexpensive high-throughput methods for phytic acid determination

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