Evaluation of QuantiFERON microtube, using 0.9 mL blood, for diagnosing tuberculosis infection

Michala V Rose; Godfather Kimaro; Inge Kroidl; Michael Hoelscher; Ib C Bygbjerg; Sayoki M Mfinanga; Pernille Ravn

doi:10.1183/09031936.00194311

Evaluation of QuantiFERON microtube, using 0.9 mL blood, for diagnosing tuberculosis infection

Michala V Rose, Godfather Kimaro, Inge Kroidl, Michael Hoelscher, Ib C Bygbjerg, Sayoki M Mfinanga, Pernille Ravn

4 Citations (Scopus)

Abstract

The performance of QuantiFERON microtube (QFT-MT), using 0.9 mL blood, and QuantiFERON-TB Gold in-tube test (QFT-IT) (3 mL blood), for diagnosing tuberculosis (TB) was compared in children and adults in an endemic setting. In 152 children with suspected TB and 87 adults with confirmed TB, QFT-IT was compared with two QFT-MT concentrations (QFT-MT A and B). Proportions of positive and indeterminate results, interferon (IFN)-γ responses, interassay agreement and sensitivity were assessed. We found similar proportions of indeterminate results, levels of IFN-γ and comparable sensitivity. The interassay agreement was moderate in all children (QFT-IT versus QFT-MT A: 85%, k50.44 and QFT-IT versus QFT-MT B: 88%, k=50.50) and adults (QFT-IT versus QFT-MT A: 88%, k50.50 and QFT-IT versus QFT-MT B: 89%, k=50.49). Sensitivity was low (QFT-IT 23%, QFTMT A 18% and B 19%) in children with confirmed or highly probable TB compared with adults (83%, 86% and 88%, respectively). The QFT-MT test can be reliably performed using less than one-third of the blood volume used in QFT-IT. The reduced volume may be useful for research and future diagnosis of paediatric TB. The poor sensitivity and high indeterminate rate of both IFN-c release assays in severely ill children, with immature or impaired immunity in an endemic setting, warrants further investigations.

Original language	English
Journal	The European Respiratory Journal
Volume	41
Issue number	4
Pages (from-to)	909-16
Number of pages	8
DOIs	https://doi.org/10.1183/09031936.00194311
Publication status	Published - 1 Apr 2013

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title = "Evaluation of QuantiFERON microtube, using 0.9 mL blood, for diagnosing tuberculosis infection",

abstract = "The performance of QuantiFERON microtube (QFT-MT), using 0.9 mL blood, and QuantiFERON-TB Gold in-tube test (QFT-IT) (3 mL blood), for diagnosing tuberculosis (TB) was compared in children and adults in an endemic setting. In 152 children with suspected TB and 87 adults with confirmed TB, QFT-IT was compared with two QFT-MT concentrations (QFT-MT A and B). Proportions of positive and indeterminate results, interferon (IFN)-γ responses, interassay agreement and sensitivity were assessed. We found similar proportions of indeterminate results, levels of IFN-γ and comparable sensitivity. The interassay agreement was moderate in all children (QFT-IT versus QFT-MT A: 85%, k50.44 and QFT-IT versus QFT-MT B: 88%, k=50.50) and adults (QFT-IT versus QFT-MT A: 88%, k50.50 and QFT-IT versus QFT-MT B: 89%, k=50.49). Sensitivity was low (QFT-IT 23%, QFTMT A 18% and B 19%) in children with confirmed or highly probable TB compared with adults (83%, 86% and 88%, respectively). The QFT-MT test can be reliably performed using less than one-third of the blood volume used in QFT-IT. The reduced volume may be useful for research and future diagnosis of paediatric TB. The poor sensitivity and high indeterminate rate of both IFN-c release assays in severely ill children, with immature or impaired immunity in an endemic setting, warrants further investigations.",

author = "Rose, {Michala V} and Godfather Kimaro and Inge Kroidl and Michael Hoelscher and Bygbjerg, {Ib C} and Mfinanga, {Sayoki M} and Pernille Ravn",

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AU - Rose, Michala V

AU - Kimaro, Godfather

AU - Kroidl, Inge

AU - Hoelscher, Michael

AU - Bygbjerg, Ib C

AU - Mfinanga, Sayoki M

AU - Ravn, Pernille

PY - 2013/4/1

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N2 - The performance of QuantiFERON microtube (QFT-MT), using 0.9 mL blood, and QuantiFERON-TB Gold in-tube test (QFT-IT) (3 mL blood), for diagnosing tuberculosis (TB) was compared in children and adults in an endemic setting. In 152 children with suspected TB and 87 adults with confirmed TB, QFT-IT was compared with two QFT-MT concentrations (QFT-MT A and B). Proportions of positive and indeterminate results, interferon (IFN)-γ responses, interassay agreement and sensitivity were assessed. We found similar proportions of indeterminate results, levels of IFN-γ and comparable sensitivity. The interassay agreement was moderate in all children (QFT-IT versus QFT-MT A: 85%, k50.44 and QFT-IT versus QFT-MT B: 88%, k=50.50) and adults (QFT-IT versus QFT-MT A: 88%, k50.50 and QFT-IT versus QFT-MT B: 89%, k=50.49). Sensitivity was low (QFT-IT 23%, QFTMT A 18% and B 19%) in children with confirmed or highly probable TB compared with adults (83%, 86% and 88%, respectively). The QFT-MT test can be reliably performed using less than one-third of the blood volume used in QFT-IT. The reduced volume may be useful for research and future diagnosis of paediatric TB. The poor sensitivity and high indeterminate rate of both IFN-c release assays in severely ill children, with immature or impaired immunity in an endemic setting, warrants further investigations.

AB - The performance of QuantiFERON microtube (QFT-MT), using 0.9 mL blood, and QuantiFERON-TB Gold in-tube test (QFT-IT) (3 mL blood), for diagnosing tuberculosis (TB) was compared in children and adults in an endemic setting. In 152 children with suspected TB and 87 adults with confirmed TB, QFT-IT was compared with two QFT-MT concentrations (QFT-MT A and B). Proportions of positive and indeterminate results, interferon (IFN)-γ responses, interassay agreement and sensitivity were assessed. We found similar proportions of indeterminate results, levels of IFN-γ and comparable sensitivity. The interassay agreement was moderate in all children (QFT-IT versus QFT-MT A: 85%, k50.44 and QFT-IT versus QFT-MT B: 88%, k=50.50) and adults (QFT-IT versus QFT-MT A: 88%, k50.50 and QFT-IT versus QFT-MT B: 89%, k=50.49). Sensitivity was low (QFT-IT 23%, QFTMT A 18% and B 19%) in children with confirmed or highly probable TB compared with adults (83%, 86% and 88%, respectively). The QFT-MT test can be reliably performed using less than one-third of the blood volume used in QFT-IT. The reduced volume may be useful for research and future diagnosis of paediatric TB. The poor sensitivity and high indeterminate rate of both IFN-c release assays in severely ill children, with immature or impaired immunity in an endemic setting, warrants further investigations.

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DO - 10.1183/09031936.00194311

M3 - Journal article

C2 - 22878880

SN - 0904-1850

VL - 41

SP - 909

EP - 916

JO - Acta tuberculosea Scandinavica

JF - Acta tuberculosea Scandinavica

IS - 4

ER -

Evaluation of QuantiFERON microtube, using 0.9 mL blood, for diagnosing tuberculosis infection

Abstract

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