Evaluation of MIC Strip Isavuconazole test for susceptibility testing of wild-type and non-wild-type Aspergillus fumigatus isolates

Maiken Cavling Arendrup; Paul Verweij; Henrik Vedel Nielsen

doi:10.1128/AAC.01659-16

Evaluation of MIC Strip Isavuconazole test for susceptibility testing of wild-type and non-wild-type Aspergillus fumigatus isolates

Maiken Cavling Arendrup^*, Paul Verweij, Henrik Vedel Nielsen

^*Corresponding author for this work

Department of Clinical Medicine

13 Citations (Scopus)

64 Downloads (Pure)

Abstract

We evaluated the MIC Strip Isavuconazole test against EUCAST E.Def 9.3 by using 40 wild-type and 39 CYP51A mutant Aspergillus fumigatus strains. The strip full inhibition endpoint (FIE) and 80% growth inhibition endpoint were determined by two independent readers, reader 1 (R1) and R2. The essential (within ±0, ±1, and ±2 twofold dilutions) and categorical agreements were best with the FIE (for R1/R2, 42%/41%, 75%/73%, and 90%/89% for essential agreement, and 91.1%/92.4% categorical agreement, with 6.3/8.9% very major errors and 0/1.3% major errors, respectively). The MIC Strip Isavuconazole test with the FIE appears to be useful.

Original language	English
Article number	e01659-16
Journal	Antimicrobial Agents and Chemotherapy
Volume	61
Issue number	1
Number of pages	5
ISSN	0066-4804
DOIs	https://doi.org/10.1128/AAC.01659-16
Publication status	Published - Jan 2017

Keywords

Antifungal susceptibility testing
Aspergillus fumigatus
Cyp51A mutants
EUCAST
Gradient strip
Isavuconazole
MIC
Wild type

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10.1128/AAC.01659-16Licence: CC BY

Evaluation of MIC Strip Isavuconazole Test for Susceptibility Testing of Wild- Type and Non-Wild-Type Aspergillus fumigatus IsolatesFinal published version, 462 KBLicence: CC BY

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title = "Evaluation of MIC Strip Isavuconazole test for susceptibility testing of wild-type and non-wild-type Aspergillus fumigatus isolates",

abstract = "We evaluated the MIC Strip Isavuconazole test against EUCAST E.Def 9.3 by using 40 wild-type and 39 CYP51A mutant Aspergillus fumigatus strains. The strip full inhibition endpoint (FIE) and 80% growth inhibition endpoint were determined by two independent readers, reader 1 (R1) and R2. The essential (within ±0, ±1, and ±2 twofold dilutions) and categorical agreements were best with the FIE (for R1/R2, 42%/41%, 75%/73%, and 90%/89% for essential agreement, and 91.1%/92.4% categorical agreement, with 6.3/8.9% very major errors and 0/1.3% major errors, respectively). The MIC Strip Isavuconazole test with the FIE appears to be useful.",

keywords = "Antifungal susceptibility testing, Aspergillus fumigatus, Cyp51A mutants, EUCAST, Gradient strip, Isavuconazole, MIC, Wild type",

author = "Arendrup, {Maiken Cavling} and Paul Verweij and Nielsen, {Henrik Vedel}",

year = "2017",

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doi = "10.1128/AAC.01659-16",

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T1 - Evaluation of MIC Strip Isavuconazole test for susceptibility testing of wild-type and non-wild-type Aspergillus fumigatus isolates

AU - Arendrup, Maiken Cavling

AU - Verweij, Paul

AU - Nielsen, Henrik Vedel

PY - 2017/1

Y1 - 2017/1

N2 - We evaluated the MIC Strip Isavuconazole test against EUCAST E.Def 9.3 by using 40 wild-type and 39 CYP51A mutant Aspergillus fumigatus strains. The strip full inhibition endpoint (FIE) and 80% growth inhibition endpoint were determined by two independent readers, reader 1 (R1) and R2. The essential (within ±0, ±1, and ±2 twofold dilutions) and categorical agreements were best with the FIE (for R1/R2, 42%/41%, 75%/73%, and 90%/89% for essential agreement, and 91.1%/92.4% categorical agreement, with 6.3/8.9% very major errors and 0/1.3% major errors, respectively). The MIC Strip Isavuconazole test with the FIE appears to be useful.

AB - We evaluated the MIC Strip Isavuconazole test against EUCAST E.Def 9.3 by using 40 wild-type and 39 CYP51A mutant Aspergillus fumigatus strains. The strip full inhibition endpoint (FIE) and 80% growth inhibition endpoint were determined by two independent readers, reader 1 (R1) and R2. The essential (within ±0, ±1, and ±2 twofold dilutions) and categorical agreements were best with the FIE (for R1/R2, 42%/41%, 75%/73%, and 90%/89% for essential agreement, and 91.1%/92.4% categorical agreement, with 6.3/8.9% very major errors and 0/1.3% major errors, respectively). The MIC Strip Isavuconazole test with the FIE appears to be useful.

KW - Antifungal susceptibility testing

KW - Aspergillus fumigatus

KW - Cyp51A mutants

KW - EUCAST

KW - Gradient strip

KW - Isavuconazole

KW - MIC

KW - Wild type

U2 - 10.1128/AAC.01659-16

DO - 10.1128/AAC.01659-16

M3 - Journal article

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AN - SCOPUS:85009238191

SN - 0066-4804

VL - 61

JO - Antimicrobial Agents and Chemotherapy

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IS - 1

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ER -

Evaluation of MIC Strip Isavuconazole test for susceptibility testing of wild-type and non-wild-type Aspergillus fumigatus isolates

Abstract

Keywords

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