Evaluation of methods to estimate production, biomass and turnover of ectomycorrhizal mycelium in forests soils: A review

H. Wallander; A. Ekblad; D.L. Godbold; D. Johnson; A. Bahr; P. Baldrian; R.G. Björk; B. Kieliszewska-Rokicka; R. Kjøller; H. Kraigher; C. Plassard; M. Rudawska

doi:10.1016/j.soilbio.2012.08.027

Evaluation of methods to estimate production, biomass and turnover of ectomycorrhizal mycelium in forests soils: A review

H. Wallander, A. Ekblad, D.L. Godbold, D. Johnson, A. Bahr, P. Baldrian, R.G. Björk, B. Kieliszewska-Rokicka, R. Kjøller, H. Kraigher, C. Plassard, M. Rudawska

Terrestrial Ecology

131 Citations (Scopus)

Abstract

Mycorrhizal fungi constitute a considerable sink for carbon in most ecosystems. This carbon is used for building extensive mycelial networks in the soil as well as for metabolic activity related to nutrient uptake. A number of methods have been developed recently to quantify production, standing biomass and turnover of extramatrical mycorrhizal mycelia (EMM) in the field. These methods include minirhizotrons, in-growth mesh bags and cores, and indirect measurements of EMM based on classification of ectomycorrhizal fungi into exploration types. Here we review the state of the art of this methodology and discuss how it can be developed and applied most effectively in the field. Furthermore, we also discuss different ways to quantify fungal biomass based on biomarkers such as chitin, ergosterol and PLFAs, as well as molecular methods, such as qPCR. The evidence thus far indicates that mycorrhizal fungi are key components of microbial biomass in many ecosystems. We highlight the need to extend the application of current methods to focus on a greater range of habitats and mycorrhizal types enabling incorporation of mycorrhizal fungal biomass and turnover into biogeochemical cycling models.

Original language	English
Journal	Soil Biology & Biochemistry
Volume	57
Pages (from-to)	1034-1047
Number of pages	14
ISSN	0038-0717
DOIs	https://doi.org/10.1016/j.soilbio.2012.08.027
Publication status	Published - Feb 2013

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Wallander, H., Ekblad, A., Godbold, D. L., Johnson, D., Bahr, A., Baldrian, P., Björk, R. G., Kieliszewska-Rokicka, B., Kjøller, R., Kraigher, H., Plassard, C., & Rudawska, M. (2013). Evaluation of methods to estimate production, biomass and turnover of ectomycorrhizal mycelium in forests soils: A review. Soil Biology & Biochemistry, 57, 1034-1047. https://doi.org/10.1016/j.soilbio.2012.08.027

Wallander, H, Ekblad, A, Godbold, DL, Johnson, D, Bahr, A, Baldrian, P, Björk, RG, Kieliszewska-Rokicka, B, Kjøller, R, Kraigher, H, Plassard, C & Rudawska, M 2013, 'Evaluation of methods to estimate production, biomass and turnover of ectomycorrhizal mycelium in forests soils: A review', Soil Biology & Biochemistry, vol. 57, pp. 1034-1047. https://doi.org/10.1016/j.soilbio.2012.08.027

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title = "Evaluation of methods to estimate production, biomass and turnover of ectomycorrhizal mycelium in forests soils: A review",

abstract = "Mycorrhizal fungi constitute a considerable sink for carbon in most ecosystems. This carbon is used for building extensive mycelial networks in the soil as well as for metabolic activity related to nutrient uptake. A number of methods have been developed recently to quantify production, standing biomass and turnover of extramatrical mycorrhizal mycelia (EMM) in the field. These methods include minirhizotrons, in-growth mesh bags and cores, and indirect measurements of EMM based on classification of ectomycorrhizal fungi into exploration types. Here we review the state of the art of this methodology and discuss how it can be developed and applied most effectively in the field. Furthermore, we also discuss different ways to quantify fungal biomass based on biomarkers such as chitin, ergosterol and PLFAs, as well as molecular methods, such as qPCR. The evidence thus far indicates that mycorrhizal fungi are key components of microbial biomass in many ecosystems. We highlight the need to extend the application of current methods to focus on a greater range of habitats and mycorrhizal types enabling incorporation of mycorrhizal fungal biomass and turnover into biogeochemical cycling models.",

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T1 - Evaluation of methods to estimate production, biomass and turnover of ectomycorrhizal mycelium in forests soils

T2 - A review

AU - Wallander, H.

AU - Ekblad, A.

AU - Godbold, D.L.

AU - Johnson, D.

AU - Bahr, A.

AU - Baldrian, P.

AU - Björk, R.G.

AU - Kieliszewska-Rokicka, B.

AU - Kjøller, R.

AU - Kraigher, H.

AU - Plassard, C.

AU - Rudawska, M.

PY - 2013/2

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N2 - Mycorrhizal fungi constitute a considerable sink for carbon in most ecosystems. This carbon is used for building extensive mycelial networks in the soil as well as for metabolic activity related to nutrient uptake. A number of methods have been developed recently to quantify production, standing biomass and turnover of extramatrical mycorrhizal mycelia (EMM) in the field. These methods include minirhizotrons, in-growth mesh bags and cores, and indirect measurements of EMM based on classification of ectomycorrhizal fungi into exploration types. Here we review the state of the art of this methodology and discuss how it can be developed and applied most effectively in the field. Furthermore, we also discuss different ways to quantify fungal biomass based on biomarkers such as chitin, ergosterol and PLFAs, as well as molecular methods, such as qPCR. The evidence thus far indicates that mycorrhizal fungi are key components of microbial biomass in many ecosystems. We highlight the need to extend the application of current methods to focus on a greater range of habitats and mycorrhizal types enabling incorporation of mycorrhizal fungal biomass and turnover into biogeochemical cycling models.

AB - Mycorrhizal fungi constitute a considerable sink for carbon in most ecosystems. This carbon is used for building extensive mycelial networks in the soil as well as for metabolic activity related to nutrient uptake. A number of methods have been developed recently to quantify production, standing biomass and turnover of extramatrical mycorrhizal mycelia (EMM) in the field. These methods include minirhizotrons, in-growth mesh bags and cores, and indirect measurements of EMM based on classification of ectomycorrhizal fungi into exploration types. Here we review the state of the art of this methodology and discuss how it can be developed and applied most effectively in the field. Furthermore, we also discuss different ways to quantify fungal biomass based on biomarkers such as chitin, ergosterol and PLFAs, as well as molecular methods, such as qPCR. The evidence thus far indicates that mycorrhizal fungi are key components of microbial biomass in many ecosystems. We highlight the need to extend the application of current methods to focus on a greater range of habitats and mycorrhizal types enabling incorporation of mycorrhizal fungal biomass and turnover into biogeochemical cycling models.

U2 - 10.1016/j.soilbio.2012.08.027

DO - 10.1016/j.soilbio.2012.08.027

M3 - Review

SN - 0038-0717

VL - 57

SP - 1034

EP - 1047

JO - Soil Biology & Biochemistry

JF - Soil Biology & Biochemistry

ER -

Evaluation of methods to estimate production, biomass and turnover of ectomycorrhizal mycelium in forests soils: A review

Abstract

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