Evaluation of flavonoids and enterolactone in overnight urine as intake biomarkers of fruits, vegetables and beverages in the Inter99 cohort study using the method of triads

Kirstine Suszkiewicz Krogholm; Anette Bysted; Anne Lise Brantsæter; Jette Halkjær Jakobsen; Salka E Rasmussen; Lis Kristoffersen; Ulla Marie Nørgaard Toft

doi:10.1017/s0007114512000104

Evaluation of flavonoids and enterolactone in overnight urine as intake biomarkers of fruits, vegetables and beverages in the Inter99 cohort study using the method of triads

Kirstine Suszkiewicz Krogholm, Anette Bysted, Anne Lise Brantsæter, Jette Halkjær Jakobsen, Salka E Rasmussen, Lis Kristoffersen, Ulla Marie Nørgaard Toft

19 Citations (Scopus)

Abstract

Since collection of 24 h urine samples is very time consuming and difficult to obtain, epidemiological studies typically only obtain spot urine samples. The aim of the present study was to evaluate whether flavonoids and enterolactone in overnight urine could substitute flavonoids and enterolactone in 24 h urine as an alternative and more feasible biomarker of fruit, vegetable and beverage intake. A total of 191 individuals in the Inter99 cohort in Denmark completed the validation study. Concentrations of nine urinary flavonoid aglycones (quercetin, isorhamnetin, tamarixetin, kaempferol, hesperetin, naringenin, eriodictyol, phloretin and apigenin) and enterolactone were determined in overnight and 24 h urine samples, and their validity as biomarkers of fruit, vegetable and beverage intake was evaluated in relation to two independent reference methods (Inter99 FFQ data and plasma carotenoids) by using the method of triads. The intakes of fruit, juice, vegetables and tea reported in the FFQ were reflected by the flavonoid biomarker both in overnight and 24 h urine samples. Validity coefficients for the flavonoid biomarker in overnight urine ranged from 0·39 to 0·49, while the corresponding validity coefficients for the biomarker in 24 h urine ranged from 0·43 to 0·66. Although the validity coefficients were lower for overnight urine than for the 24 h urine flavonoid biomarker, they were still of acceptable magnitude. In conclusion, the results indicate that flavonoids and enterolactone in overnight urine samples may be used as a more feasible biomarker than 24 h urine for the assessment and validation of fruit, juice, vegetable and tea intakes in epidemiological studies.

Original language	English
Journal	The British Journal of Nutrition
Volume	108
Issue number	10
Pages (from-to)	1904-12
Number of pages	9
ISSN	0007-1145
DOIs	https://doi.org/10.1017/s0007114512000104
Publication status	Published - 28 Nov 2012

Keywords

4-Butyrolactone
Adult
Cohort Studies
Diet
Female
Flavonoids
Food Habits
Fruit
Humans
Lignans
Male
Middle Aged
Models, Biological
Vegetables

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10.1017/s0007114512000104

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Krogholm, K. S., Bysted, A., Brantsæter, A. L., Jakobsen, J. H., Rasmussen, S. E., Kristoffersen, L., & Toft, U. M. N. (2012). Evaluation of flavonoids and enterolactone in overnight urine as intake biomarkers of fruits, vegetables and beverages in the Inter99 cohort study using the method of triads. The British Journal of Nutrition, 108(10), 1904-12. https://doi.org/10.1017/s0007114512000104

Evaluation of flavonoids and enterolactone in overnight urine as intake biomarkers of fruits, vegetables and beverages in the Inter99 cohort study using the method of triads. / Krogholm, Kirstine Suszkiewicz; Bysted, Anette; Brantsæter, Anne Lise et al.
In: The British Journal of Nutrition, Vol. 108, No. 10, 28.11.2012, p. 1904-12.

Research output: Contribution to journal › Journal article › Research › peer-review

Krogholm, KS, Bysted, A, Brantsæter, AL, Jakobsen, JH, Rasmussen, SE, Kristoffersen, L & Toft, UMN 2012, 'Evaluation of flavonoids and enterolactone in overnight urine as intake biomarkers of fruits, vegetables and beverages in the Inter99 cohort study using the method of triads', The British Journal of Nutrition, vol. 108, no. 10, pp. 1904-12. https://doi.org/10.1017/s0007114512000104

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title = "Evaluation of flavonoids and enterolactone in overnight urine as intake biomarkers of fruits, vegetables and beverages in the Inter99 cohort study using the method of triads",

abstract = "Since collection of 24 h urine samples is very time consuming and difficult to obtain, epidemiological studies typically only obtain spot urine samples. The aim of the present study was to evaluate whether flavonoids and enterolactone in overnight urine could substitute flavonoids and enterolactone in 24 h urine as an alternative and more feasible biomarker of fruit, vegetable and beverage intake. A total of 191 individuals in the Inter99 cohort in Denmark completed the validation study. Concentrations of nine urinary flavonoid aglycones (quercetin, isorhamnetin, tamarixetin, kaempferol, hesperetin, naringenin, eriodictyol, phloretin and apigenin) and enterolactone were determined in overnight and 24 h urine samples, and their validity as biomarkers of fruit, vegetable and beverage intake was evaluated in relation to two independent reference methods (Inter99 FFQ data and plasma carotenoids) by using the method of triads. The intakes of fruit, juice, vegetables and tea reported in the FFQ were reflected by the flavonoid biomarker both in overnight and 24 h urine samples. Validity coefficients for the flavonoid biomarker in overnight urine ranged from 0·39 to 0·49, while the corresponding validity coefficients for the biomarker in 24 h urine ranged from 0·43 to 0·66. Although the validity coefficients were lower for overnight urine than for the 24 h urine flavonoid biomarker, they were still of acceptable magnitude. In conclusion, the results indicate that flavonoids and enterolactone in overnight urine samples may be used as a more feasible biomarker than 24 h urine for the assessment and validation of fruit, juice, vegetable and tea intakes in epidemiological studies.",

keywords = "4-Butyrolactone, Adult, Cohort Studies, Diet, Female, Flavonoids, Food Habits, Fruit, Humans, Lignans, Male, Middle Aged, Models, Biological, Vegetables",

author = "Krogholm, {Kirstine Suszkiewicz} and Anette Bysted and Brants{\ae}ter, {Anne Lise} and Jakobsen, {Jette Halkj{\ae}r} and Rasmussen, {Salka E} and Lis Kristoffersen and Toft, {Ulla Marie N{\o}rgaard}",

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T1 - Evaluation of flavonoids and enterolactone in overnight urine as intake biomarkers of fruits, vegetables and beverages in the Inter99 cohort study using the method of triads

AU - Krogholm, Kirstine Suszkiewicz

AU - Bysted, Anette

AU - Brantsæter, Anne Lise

AU - Jakobsen, Jette Halkjær

AU - Rasmussen, Salka E

AU - Kristoffersen, Lis

AU - Toft, Ulla Marie Nørgaard

PY - 2012/11/28

Y1 - 2012/11/28

N2 - Since collection of 24 h urine samples is very time consuming and difficult to obtain, epidemiological studies typically only obtain spot urine samples. The aim of the present study was to evaluate whether flavonoids and enterolactone in overnight urine could substitute flavonoids and enterolactone in 24 h urine as an alternative and more feasible biomarker of fruit, vegetable and beverage intake. A total of 191 individuals in the Inter99 cohort in Denmark completed the validation study. Concentrations of nine urinary flavonoid aglycones (quercetin, isorhamnetin, tamarixetin, kaempferol, hesperetin, naringenin, eriodictyol, phloretin and apigenin) and enterolactone were determined in overnight and 24 h urine samples, and their validity as biomarkers of fruit, vegetable and beverage intake was evaluated in relation to two independent reference methods (Inter99 FFQ data and plasma carotenoids) by using the method of triads. The intakes of fruit, juice, vegetables and tea reported in the FFQ were reflected by the flavonoid biomarker both in overnight and 24 h urine samples. Validity coefficients for the flavonoid biomarker in overnight urine ranged from 0·39 to 0·49, while the corresponding validity coefficients for the biomarker in 24 h urine ranged from 0·43 to 0·66. Although the validity coefficients were lower for overnight urine than for the 24 h urine flavonoid biomarker, they were still of acceptable magnitude. In conclusion, the results indicate that flavonoids and enterolactone in overnight urine samples may be used as a more feasible biomarker than 24 h urine for the assessment and validation of fruit, juice, vegetable and tea intakes in epidemiological studies.

AB - Since collection of 24 h urine samples is very time consuming and difficult to obtain, epidemiological studies typically only obtain spot urine samples. The aim of the present study was to evaluate whether flavonoids and enterolactone in overnight urine could substitute flavonoids and enterolactone in 24 h urine as an alternative and more feasible biomarker of fruit, vegetable and beverage intake. A total of 191 individuals in the Inter99 cohort in Denmark completed the validation study. Concentrations of nine urinary flavonoid aglycones (quercetin, isorhamnetin, tamarixetin, kaempferol, hesperetin, naringenin, eriodictyol, phloretin and apigenin) and enterolactone were determined in overnight and 24 h urine samples, and their validity as biomarkers of fruit, vegetable and beverage intake was evaluated in relation to two independent reference methods (Inter99 FFQ data and plasma carotenoids) by using the method of triads. The intakes of fruit, juice, vegetables and tea reported in the FFQ were reflected by the flavonoid biomarker both in overnight and 24 h urine samples. Validity coefficients for the flavonoid biomarker in overnight urine ranged from 0·39 to 0·49, while the corresponding validity coefficients for the biomarker in 24 h urine ranged from 0·43 to 0·66. Although the validity coefficients were lower for overnight urine than for the 24 h urine flavonoid biomarker, they were still of acceptable magnitude. In conclusion, the results indicate that flavonoids and enterolactone in overnight urine samples may be used as a more feasible biomarker than 24 h urine for the assessment and validation of fruit, juice, vegetable and tea intakes in epidemiological studies.

KW - 4-Butyrolactone

KW - Adult

KW - Cohort Studies

KW - Diet

KW - Female

KW - Flavonoids

KW - Food Habits

KW - Fruit

KW - Humans

KW - Lignans

KW - Male

KW - Middle Aged

KW - Models, Biological

KW - Vegetables

U2 - 10.1017/s0007114512000104

DO - 10.1017/s0007114512000104

M3 - Journal article

C2 - 22453033

SN - 0007-1145

VL - 108

SP - 1904

EP - 1912

JO - The British Journal of Nutrition

JF - The British Journal of Nutrition

IS - 10

ER -

Evaluation of flavonoids and enterolactone in overnight urine as intake biomarkers of fruits, vegetables and beverages in the Inter99 cohort study using the method of triads

Abstract

Keywords

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