Evaluation of different toxicity assays applied to proliferating cells and to stratified epithelium in relation to permeability enhancement with glycocholate

Heidi Ugelstad Eirheim; Christoffer Bundgaard; Hanne Mørck Nielsen

doi:10.1016/j.tiv.2004.02.003

Evaluation of different toxicity assays applied to proliferating cells and to stratified epithelium in relation to permeability enhancement with glycocholate

Heidi Ugelstad Eirheim, Christoffer Bundgaard, Hanne Mørck Nielsen

27 Citations (Scopus)

Abstract

The purpose of the present study was to evaluate different toxicity assays for use on proliferating buccal TR146 cells and on stratified TR146 epithelium and to compare these results to the permeability enhancing effect of glycocholate (GC). Both the proliferating cells and the epithelium were exposed to different GC concentrations for 4 h. The MTS/PMS assay and neutral red (NR) retention were performed along with quantitation of ATP, lactate dehydrogenase (LDH) and extracellular protein. The toxicity was calculated as the IC50 value relative to the control. Increase in 3H-mannitol permeability across the epithelium concurrent with a decrease in the transepithelial electrical resistance (TEER) was also determined. The robustness of the epithelium was significantly higher than that of the proliferating cells (P

Original language	English
Journal	Toxicology in Vitro
Volume	18
Issue number	5
Pages (from-to)	649-57
Number of pages	9
ISSN	0887-2333
DOIs	https://doi.org/10.1016/j.tiv.2004.02.003
Publication status	Published - 2004

Keywords

Adenosine Triphosphate
Cell Line, Tumor
Cell Membrane Permeability
Cell Proliferation
Detergents
Dose-Response Relationship, Drug
Epithelial Cells
Glycocholic Acid
Humans
Inhibitory Concentration 50
Mannitol
Methylphenazonium Methosulfate
Mouth Mucosa
Neutral Red
Proteins
Reproducibility of Results
Tetrazolium Salts
Thiazoles
Toxicity Tests

Access to Document

10.1016/j.tiv.2004.02.003

Cite this

Evaluation of different toxicity assays applied to proliferating cells and to stratified epithelium in relation to permeability enhancement with glycocholate. / Eirheim, Heidi Ugelstad; Bundgaard, Christoffer; Nielsen, Hanne Mørck.

In: Toxicology in Vitro, Vol. 18, No. 5, 2004, p. 649-57.

Research output: Contribution to journal › Journal article › Research › peer-review

@article{159af813001e4c38a895a3cae36acf3e,

title = "Evaluation of different toxicity assays applied to proliferating cells and to stratified epithelium in relation to permeability enhancement with glycocholate",

abstract = "The purpose of the present study was to evaluate different toxicity assays for use on proliferating buccal TR146 cells and on stratified TR146 epithelium and to compare these results to the permeability enhancing effect of glycocholate (GC). Both the proliferating cells and the epithelium were exposed to different GC concentrations for 4 h. The MTS/PMS assay and neutral red (NR) retention were performed along with quantitation of ATP, lactate dehydrogenase (LDH) and extracellular protein. The toxicity was calculated as the IC50 value relative to the control. Increase in 3H-mannitol permeability across the epithelium concurrent with a decrease in the transepithelial electrical resistance (TEER) was also determined. The robustness of the epithelium was significantly higher than that of the proliferating cells (P ",

keywords = "Adenosine Triphosphate, Cell Line, Tumor, Cell Membrane Permeability, Cell Proliferation, Detergents, Dose-Response Relationship, Drug, Epithelial Cells, Glycocholic Acid, Humans, Inhibitory Concentration 50, Mannitol, Methylphenazonium Methosulfate, Mouth Mucosa, Neutral Red, Proteins, Reproducibility of Results, Tetrazolium Salts, Thiazoles, Toxicity Tests",

author = "Eirheim, {Heidi Ugelstad} and Christoffer Bundgaard and Nielsen, {Hanne M{\o}rck}",

year = "2004",

doi = "10.1016/j.tiv.2004.02.003",

language = "English",

volume = "18",

pages = "649--57",

journal = "Toxicology in Vitro",

issn = "0887-2333",

publisher = "Pergamon Press",

number = "5",

}

TY - JOUR

T1 - Evaluation of different toxicity assays applied to proliferating cells and to stratified epithelium in relation to permeability enhancement with glycocholate

AU - Eirheim, Heidi Ugelstad

AU - Bundgaard, Christoffer

AU - Nielsen, Hanne Mørck

PY - 2004

Y1 - 2004

N2 - The purpose of the present study was to evaluate different toxicity assays for use on proliferating buccal TR146 cells and on stratified TR146 epithelium and to compare these results to the permeability enhancing effect of glycocholate (GC). Both the proliferating cells and the epithelium were exposed to different GC concentrations for 4 h. The MTS/PMS assay and neutral red (NR) retention were performed along with quantitation of ATP, lactate dehydrogenase (LDH) and extracellular protein. The toxicity was calculated as the IC50 value relative to the control. Increase in 3H-mannitol permeability across the epithelium concurrent with a decrease in the transepithelial electrical resistance (TEER) was also determined. The robustness of the epithelium was significantly higher than that of the proliferating cells (P

AB - The purpose of the present study was to evaluate different toxicity assays for use on proliferating buccal TR146 cells and on stratified TR146 epithelium and to compare these results to the permeability enhancing effect of glycocholate (GC). Both the proliferating cells and the epithelium were exposed to different GC concentrations for 4 h. The MTS/PMS assay and neutral red (NR) retention were performed along with quantitation of ATP, lactate dehydrogenase (LDH) and extracellular protein. The toxicity was calculated as the IC50 value relative to the control. Increase in 3H-mannitol permeability across the epithelium concurrent with a decrease in the transepithelial electrical resistance (TEER) was also determined. The robustness of the epithelium was significantly higher than that of the proliferating cells (P

KW - Adenosine Triphosphate

KW - Cell Line, Tumor

KW - Cell Membrane Permeability

KW - Cell Proliferation

KW - Detergents

KW - Dose-Response Relationship, Drug

KW - Epithelial Cells

KW - Glycocholic Acid

KW - Humans

KW - Inhibitory Concentration 50

KW - Mannitol

KW - Methylphenazonium Methosulfate

KW - Mouth Mucosa

KW - Neutral Red

KW - Proteins

KW - Reproducibility of Results

KW - Tetrazolium Salts

KW - Thiazoles

KW - Toxicity Tests

U2 - 10.1016/j.tiv.2004.02.003

DO - 10.1016/j.tiv.2004.02.003

M3 - Journal article

C2 - 15251183

SN - 0887-2333

VL - 18

SP - 649

EP - 657

JO - Toxicology in Vitro

JF - Toxicology in Vitro

IS - 5

ER -

Evaluation of different toxicity assays applied to proliferating cells and to stratified epithelium in relation to permeability enhancement with glycocholate

Abstract

Keywords

Access to Document

Fingerprint

Cite this