Evaluation of acute tryptophan depletion and sham depletion with a gelatin-based collagen peptide protein mixture

TY - JOUR

T1 - Evaluation of acute tryptophan depletion and sham depletion with a gelatin-based collagen peptide protein mixture

AU - Stenbæk, Dea Siggaard

AU - Einarsdottir, H S

AU - Goregliad-Fjaellingsdal, T

AU - Knudsen, Gitte M.

AU - Frokjaer, V. G.

AU - Hasselbalch, S G

N1 - Copyright © 2015 Elsevier B.V. and ECNP. All rights reserved.

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Acute Tryptophan Depletion (ATD) is a dietary method used to modulate central 5-HT to study the effects of temporarily reduced 5-HT synthesis. The aim of this study is to evaluate a novel method of ATD using a gelatin-based collagen peptide (CP) mixture. We administered CP-Trp or CP+Trp mixtures to 29 healthy volunteers; 13 from a randomized, double-blinded crossover study and sixteen from a randomized, double-blinded study run in our laboratory. Plasma amino acids, mood, side effects, cortisol concentrations, mean arterial blood pressure and heart rate were measured at multiple time-points. Repeated measures analysis of variance and Wilcoxon or Mann-Whitney U non-parametric tests were used to analyze the effects of intervention. Intake of the CP-Trp mixture efficiently reduced plasma Trp; however, the CP+Trp mixture induced a large significant increase in plasma Trp. No other significant effects of CP-Trp compared to CP+Trp were observed. The transient increase in plasma Trp after CP+Trp may impair comparison to the CP-Trp and we therefore recommend in future studies to use a smaller dose of Trp supplement to the CP mixture.

AB - Acute Tryptophan Depletion (ATD) is a dietary method used to modulate central 5-HT to study the effects of temporarily reduced 5-HT synthesis. The aim of this study is to evaluate a novel method of ATD using a gelatin-based collagen peptide (CP) mixture. We administered CP-Trp or CP+Trp mixtures to 29 healthy volunteers; 13 from a randomized, double-blinded crossover study and sixteen from a randomized, double-blinded study run in our laboratory. Plasma amino acids, mood, side effects, cortisol concentrations, mean arterial blood pressure and heart rate were measured at multiple time-points. Repeated measures analysis of variance and Wilcoxon or Mann-Whitney U non-parametric tests were used to analyze the effects of intervention. Intake of the CP-Trp mixture efficiently reduced plasma Trp; however, the CP+Trp mixture induced a large significant increase in plasma Trp. No other significant effects of CP-Trp compared to CP+Trp were observed. The transient increase in plasma Trp after CP+Trp may impair comparison to the CP-Trp and we therefore recommend in future studies to use a smaller dose of Trp supplement to the CP mixture.

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.euroneuro.2015.11.010

DO - 10.1016/j.euroneuro.2015.11.010

M3 - Letter

C2 - 26655163

SN - 0924-977X

VL - 26

SP - 147

EP - 149

JO - European Neuropsychopharmacology

JF - European Neuropsychopharmacology

IS - 1

ER -