Evaluating the potential of IP-10 and MCP-2 as biomarkers for the diagnosis of TB.

TY - JOUR

T1 - Evaluating the potential of IP-10 and MCP-2 as biomarkers for the diagnosis of TB.

AU - Ruhwald, Morten

AU - Bodmer, T

AU - Maier, C

AU - Jepsen, M

AU - Andersen, M B

AU - Eugen-Olsen, Jesper

AU - Ravn, Pernille

AU - NN, NN

PY - 2008

Y1 - 2008

N2 - The aim of the study was to evaluate the potential of diagnostic tests based on IP-10 and MCP-2, and compare the performance with the Quantiferon-Gold In-Tube (QFT-IT) test.IP-10 and MCP-2 were determined in supernatants from whole blood stimulated with M.tuberculosis-specific antigens. Samples were obtained from 80 patients with culture and/or PCR proven TB, and 124 unexposed healthy controls; 86 high school students and 38 high school staff. IP-10 and MCP-2 test cut-offs were established based on ROC curve analysis.TB patients produced significantly higher levels of IP-10 (median 2158 pg.ml(-1)) and MCP-2 (median 379 pg.ml(-1)) compared with IFN-gamma (median 215 pg.ml(-1), p<0.0001). The QFT-IT, IP-10 and MCP-2 tests detected 81%, 83% and 71% of the TB patients; 0%, 3% and 0% of the high school students and 0%, 16%; and 3% of the staff. Agreement between tests was high >89% (kappa>0.77). By combining IP-10 and IFN-gamma tests the detection rate increased among TB patients to 90% without a significant increase in positive responders among the students.In conclusion, IP-10 and MCP-2 responses to M.tuberculosis specific antigens could be used to diagnose infection. Combining IP-10 and IFN-gamma may be a simple approach to increase the detection rate of the M.tuberculosis specific in-vitro tests.

AB - The aim of the study was to evaluate the potential of diagnostic tests based on IP-10 and MCP-2, and compare the performance with the Quantiferon-Gold In-Tube (QFT-IT) test.IP-10 and MCP-2 were determined in supernatants from whole blood stimulated with M.tuberculosis-specific antigens. Samples were obtained from 80 patients with culture and/or PCR proven TB, and 124 unexposed healthy controls; 86 high school students and 38 high school staff. IP-10 and MCP-2 test cut-offs were established based on ROC curve analysis.TB patients produced significantly higher levels of IP-10 (median 2158 pg.ml(-1)) and MCP-2 (median 379 pg.ml(-1)) compared with IFN-gamma (median 215 pg.ml(-1), p<0.0001). The QFT-IT, IP-10 and MCP-2 tests detected 81%, 83% and 71% of the TB patients; 0%, 3% and 0% of the high school students and 0%, 16%; and 3% of the staff. Agreement between tests was high >89% (kappa>0.77). By combining IP-10 and IFN-gamma tests the detection rate increased among TB patients to 90% without a significant increase in positive responders among the students.In conclusion, IP-10 and MCP-2 responses to M.tuberculosis specific antigens could be used to diagnose infection. Combining IP-10 and IFN-gamma may be a simple approach to increase the detection rate of the M.tuberculosis specific in-vitro tests.

U2 - 10.1183/09031936.00055508

DO - 10.1183/09031936.00055508

M3 - Journal article

SN - 0903-1936

VL - 32

SP - 1607

EP - 1615

JO - European Respiratory Journal

JF - European Respiratory Journal

IS - 6

ER -