Establishing a synthetic pathway for high-level production of 3-hydroxypropionic acid in Saccharomyces cerevisiae via β-alanine

TY - JOUR

T1 - Establishing a synthetic pathway for high-level production of 3-hydroxypropionic acid in Saccharomyces cerevisiae via β-alanine

AU - Borodina, Irina

AU - Kildegaard, Kanchana R

AU - Jensen, Niels B

AU - Blicher, Thomas H

AU - Maury, Jérôme

AU - Sherstyk, Svetlana

AU - Schneider, Konstantin

AU - Lamosa, Pedro

AU - Herrgård, Markus J

AU - Rosenstand, Inger

AU - Oberg, Fredrik

AU - Forster, Jochen

AU - Nielsen, Jens

N1 - Copyright © 2014 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Microbial fermentation of renewable feedstocks into plastic monomers can decrease our fossil dependence and reduce global CO2 emissions. 3-Hydroxypropionic acid (3HP) is a potential chemical building block for sustainable production of superabsorbent polymers and acrylic plastics. With the objective of developing Saccharomyces cerevisiae as an efficient cell factory for high-level production of 3HP, we identified the β-alanine biosynthetic route as the most economically attractive according to the metabolic modeling. We engineered and optimized a synthetic pathway for de novo biosynthesis of β-alanine and its subsequent conversion into 3HP using a novel β-alanine-pyruvate aminotransferase discovered in Bacillus cereus. The final strain produced 3HP at a titer of 13.7±0.3gL(-1) with a 0.14±0.0C-molC-mol(-1) yield on glucose in 80h in controlled fed-batch fermentation in mineral medium at pH 5, and this work therefore lays the basis for developing a process for biological 3HP production.

AB - Microbial fermentation of renewable feedstocks into plastic monomers can decrease our fossil dependence and reduce global CO2 emissions. 3-Hydroxypropionic acid (3HP) is a potential chemical building block for sustainable production of superabsorbent polymers and acrylic plastics. With the objective of developing Saccharomyces cerevisiae as an efficient cell factory for high-level production of 3HP, we identified the β-alanine biosynthetic route as the most economically attractive according to the metabolic modeling. We engineered and optimized a synthetic pathway for de novo biosynthesis of β-alanine and its subsequent conversion into 3HP using a novel β-alanine-pyruvate aminotransferase discovered in Bacillus cereus. The final strain produced 3HP at a titer of 13.7±0.3gL(-1) with a 0.14±0.0C-molC-mol(-1) yield on glucose in 80h in controlled fed-batch fermentation in mineral medium at pH 5, and this work therefore lays the basis for developing a process for biological 3HP production.

U2 - 10.1016/j.ymben.2014.10.003

DO - 10.1016/j.ymben.2014.10.003

M3 - Journal article

C2 - 25447643

SN - 1096-7176

VL - 27

SP - 57

EP - 64

JO - Metabolic Engineering

JF - Metabolic Engineering

ER -